Combined endurance and resistance exercise training alters the spatial transcriptome of skeletal muscle in young adults.

Chronic exercise training substantially improves skeletal muscle function and performance. The repeated demands and stressors of each exercise bout drive coordinated molecular adaptations within multiple cell types, leading to enhanced neuromuscular recruitment and contractile function, stem cell activation, myofiber hypertrophy, mitochondrial biogenesis, and angiogenesis, among others. To comprehensively profile molecular changes induced by combined resistance and endurance exercise training, we employed spatial transcriptomics coupled with immunofluorescence and computational approaches to resolve effects on myofiber and mononuclear cell populations in human muscle.

Machine Learning Enables Rapid Detection of Slips Using a Robotic Hip Exoskeleton.

Fall incidents due to slips are some of the most common causes of injuries for industry workers and older adults, motivating research to assist balance recovery following slips. To assist balance recovery during a slip, a detection algorithm that can work with an assistive device, such as an exoskeleton, needs to be able to detect slips rapidly after onset, which remains a critical gap in the field. Here, we compared the ability of linear discriminant analysis (LDA), extreme gradient boosting (XGBoost), and convolutional neural networks (CNN) to detect slip using only native sensors on a hip exoskeleton.

Evaluation of the Agreement Between Research-Grade Actigraphy Sleep, Consumer-Grade Smartwatches and Self-Reported Sleep Diaries in Masters Endurance Athletes.

Sleep monitoring is a tool widely used to support recovery and performance in endurance athletes. This study aimed to assess agreement between research-grade actigraphy (ActiGraph GT9X), consumer-grade smartwatches (Garmin), and self-reported sleep diaries in masters endurance athletes. Seventy athletes (43 males, 46.

Molecular signatures underlying heterogenous hypertrophy responsiveness to resistance training in older men and women: a within-subject design.

We showed higher resistance training (RT) volume (4-sets) offsets muscle hypertrophy non-responsiveness to single-set RT (1-set), while enhancing hypertrophic response among responders. Here, we investigated molecular traits underpinning RT-related hypertrophic variability via targeted (ribosome density and biogenesis) and untargeted approaches (RNAseq gene-ontology (GO) and Pathway-Level Information ExtractoR [PLIER]). Twenty-seven older (693 years) participants in the parental trial had one leg randomly allocated to 1-set and the contralateral leg allocated to 4-sets of RT for 10-wk, 2x/week.

Cognitively-plausible reinforcement learning in epidemiological agent-based simulations.

Introduction: Human behavior shapes the transmission of infectious diseases and determines the effectiveness of public health measures designed to mitigate transmission. To accurately reflect these dynamics, epidemiological simulation models should endogenously account for both disease transmission and behavioral dynamics. Traditional agent-based models (ABMs) often rely on simplified rules to represent behavior, limiting their ability to capture complex decision-making processes and cognitive dynamics.

Consensus Statements-Optimizing Performance of the Elite Athlete.

The International Consensus Conference "Optimising Performance of the Elite Athlete," held in November 2024, brought together 29 scientists, some coaches, and athletes to establish evidence-based consensus statements aimed at enhancing elite athletic performance and health. The conference addressed critical themes including training strategies, nutrition, female athlete considerations, injury management, and emerging technologies. Key conclusions emphasize individualized, sport-specific approaches to training and nutrition, integrating concurrent training modalities to improve endurance, resilience, and efficiency.

Multidimensional biocircuitry of exercise adaptation: integrating in vivo and ex vivo phenomics with miRNA mapping.

In a randomized, dose-response trial, we used molecular and phenomic profiling to compare responses with traditional moderate-intensity endurance and resistance training (TRAD) versus high-intensity tactical training (HITT) that encompassed explosive whole-body interval training and high-intensity resistance training. Ninety-four participants (18-27 yr) completed 12 wk of TRAD or HITT followed by 4 wk of detraining. Although similar performance and body composition improvements were observed in response to HITT and TRAD, some dose-dependent differences were observed for: ) ex vivo muscle tissue changes in myofiber size, capillarization, satellite cell frequency, and mitochondrial function and ) differential gene expression (DGE) of muscle and serum exosomal miRNAs (miRs).

Multidimensional Modeling to Maximize Adaptations to eXercise: The MAX Trial Rationale and Study Design.

Age-related functional declines are thought to be caused by hallmark biological processes that manifest in physical, mental, and metabolic impairments compromising intrinsic capacity, healthspan and quality-of-life. Exercise is a multipotent treatment with promise to mitigate most aging hallmarks, but there is substantial variability in individual exercise responsiveness. This inter-individual response heterogeneity (IRH) was first extensively interrogated by Bouchard and colleagues in the context of endurance training.

Prediction of U.S. daily mask wearing and social distancing using psychologically valid agents during three waves of COVID-19.

We present Regional Psychologically Valid Agents (R-PVAs) as a modeling approach to predicting transmission-reducing behaviors and epidemiology. The approach builds upon computational cognitive theory and formalizes aspects of theories of individual-level behavior change. We present R-PVA models of social distancing and mask wearing in response to dynamics in the physical and information environments in the 50 U.

Less than 4% of participants in studies of carbohydrate-based fuelling strategies for soccer performance have been female: results from an audit of her representation.

Women's soccer has increased markedly in popularity and professionalisation in recent years. Achieving adequate carbohydrate (CHO) availability before and during performance is often advised as a key priority for players. The aim of this study was to audit the literature that informs the current CHO-based guidelines for soccer performance and to assess the extent of evidence supporting their application to female players.

Engaging Stakeholders Using the Competency Matrix Model: A Framework for Current and Future Health Informaticists.

Objectives:  This study aims to explore how health informaticists collaborate with multiple stakeholder groups, each possessing varying levels of comfort and competence with health technology and data. stakeholder engagement is highlighted as a crucial skill for health informaticists, necessitated by the differing competency levels among stakeholders.

Methods:  The Competency Matrix Model was identified as a strategic tool to address the challenges faced by health informaticists in navigating the complexities of health information technology utilization.

Muscle transcriptome profiles in elite male ultra-endurance athletes acclimated to a high-carbohydrate versus low-carbohydrate diet.

Low-carbohydrate, high-fat diets enhance lipid metabolism and decrease reliance on glucose oxidation in athletes, but the associated gene expression patterns remain unclear. The purpose of this study was to determine whether coordinated molecular pathways in skeletal muscle may be revealed by differential expression of genes driven by dietary profile, exercise, and/or their interaction. We investigated the skeletal muscle transcriptome in elite ultra-endurance athletes habitually (~ 20 months) consuming a high-carbohydrate, low-fat (HC, n = 10, 33 ± 6y, VO2max = 63.

Multisystem impact of altering acid load of ingested exogenous ketone supplements at rest in young healthy adults.

Disruptions to acid-base are observed in extreme environments as well as respiratory and metabolic diseases. Exogenous ketone supplements (EKSs) have been proposed to mitigate these processes and provide therapeutic benefits by altering acid-base balance and metabolism, but direct comparison of various forms of EKS is lacking. Twenty healthy participants (M/F: 10/10; age: 20.

Divergent multiomic acute exercise responses reveal the impact of sex as a biological variable.

The majority of exercise physiology research has been conducted in males, resulting in a skewed biological representation of how exercise impacts the physiological system. Extrapolating male-centric physiological findings to females is not universally appropriate and may even be detrimental. Thus, addressing this imbalance and taking into consideration sex as a biological variable is mandatory for optimization of precision exercise interventions and/or regimens.

Acute high-intensity exercise enhances T cell proliferation compared to moderate-intensity exercise.

Conventional belief is that high-intensity (HI) exercise inhibits immune function; however, recent work challenges this position. The purpose of this was to quantify changes in T cell proliferative capacity following either a HI or moderate-intensity (MI) exercise. Sixteen males were randomly selected to a HI or MI exercise group.

Effects of leg immobilization and recovery resistance training on skeletal muscle-molecular markers in previously resistance-trained versus untrained adults.

We sought to examine how resistance training (RT) status in young healthy individuals, either well resistance trained (T, = 10) or untrained (UT, = 11), affected molecular markers with leg immobilization followed by recovery RT. All participants underwent 2 wk of left leg immobilization via a locking leg brace. Afterward, all participants underwent 8 wk (3 days/wk) of knee extensor-focused progressive RT.

Exercise intensity and training alter the innate immune cell type and chromosomal origins of circulating cell-free DNA in humans.

Exercising regularly promotes health, but these benefits are complicated by acute inflammation induced by exercise. A potential source of inflammation is cell-free DNA (cfDNA), yet the cellular origins, molecular causes, and immune system interactions of exercise-induced cfDNA are unclear. To study these, 10 healthy individuals were randomized to a 12-wk exercise program of either high-intensity tactical training (HITT) or traditional moderate-intensity training (TRAD).

Carbohydrate ingestion eliminates hypoglycemia and improves endurance exercise performance in triathletes adapted to very low- and high-carbohydrate isocaloric diets.

Very-low-carbohydrate diets (LCHF; <50 g/day) have been debated for their potential to lower pre-exercise muscle and liver glycogen stores and metabolic efficiency, risking premature fatigue. It is also hypothesized that carbohydrate ingestion during prolonged exercise delays fatigue by increasing carbohydrate oxidation, thereby sparing muscle glycogen. Leveraging a randomized crossover design, we evaluated performance during strenuous time-to-exhaustion (70% V̇o) tests in trained triathletes following 6-wk high-carbohydrate (HCLF, 380 g/day) or very-low-carbohydrate (LCHF, 40 g/day) diets to determine ) if adoption of the LCHF diet impairs time-to-exhaustion performance, ) whether carbohydrate ingestion (10 g/h) 6-12× lower than current CHO fueling recommendations during low glycogen availability (>15-h pre-exercise overnight fast and/or LCHF diet) improves time to exhaustion by preventing exercise-induced hypoglycemia (EIH; <3.

The impact of risk compensation adaptive behavior on the final epidemic size.

Public health interventions reduce infection risk, while imposing significant costs on both individuals and the society. Interventions can also lead to behavioral changes, as individuals weigh the cost and benefits of avoiding infection. Aggregate epidemiological models typically focus on the population-level consequences of interventions, often not incorporating the mechanisms driving behavioral adaptations associated with interventions compliance.

Muscle-restricted knockout of connexin 43 and connexin 45 accelerates and improves locomotor recovery after contusion spinal cord injury.

Traumatic spinal cord injury (SCI) results in the disruption of physiological systems below the level of the spinal lesion. Connexin hemichannels (CxHCs) are membrane-bound, non-selective pore proteins that are lost in mature myofibers but reappear on the sarcolemma after peripheral denervation, chronic SCI, diabetes, and severe systemic stress such as sepsis. Cx43 and Cx45 have been implicated as the major CxHCs present in diseased muscle, and muscle-restricted knockout of these genes reduces muscle atrophy after denervation, likely by reducing excess calcium influx with resultant inflammasome activation.

Postexercise Dietary Leucine Retention for Whole-Body Anabolism Is Greater With Whey Protein Isolate and Fish-Derived Protein Hydrolysate Than Nonessential Amino Acids in Trained Young Men.

Marine-derived proteins, such as blue whiting-derived protein hydrolysates (BWPH), represent high-quality sources of dietary protein, but their ability to support postexercise anabolism is not established. The impact of BWPH on whole-body anabolism was compared with an isonitrogenous whey protein isolate (WPI) and nonessential amino acid (NEAA) control in 10 trained young males (31 ± 4 years) who, on three separate visits, performed a session of whole-body resistance exercise and then consumed, in randomized crossover fashion, BWPH, WPI, or NEAA (0.33 g/kg; 19, 33, and 0 mg/kg leucine, respectively) with L-[1-13C]leucine.

Pharmacology of boldine: summary of the field and update on recent advances.

Over the past decade, boldine, a naturally occurring alkaloid found in several plant species including the Chilean Boldo tree, has garnered attention for its efficacy in rodent models of human disease. Some of the properties that have been attributed to boldine include antioxidant activities, neuroprotective and analgesic actions, hepatoprotective effects, anti-inflammatory actions, cardioprotective effects and anticancer potential. Compelling data now indicates that boldine blocks connexin (Cx) hemichannels (HCs) and that many if not all of its effects in rodent models of injury and disease are due to CxHC blockade.

Ketone monoester attenuates declines in cognitive performance and oxygen saturation during acute severe hypoxic exposure under resting conditions.

Exogenous ketone supplements are a potential augmentation strategy for cognitive resilience during acute hypoxic exposure due to their capacity to attenuate the decline in oxygen (O) availability, and by providing an alternative substrate for cerebral metabolism. Utilizing a single-blind randomized crossover design, 16 male military personnel (age, 25.3 ± 2.

Ketone monoester attenuates oxygen desaturation during weighted ruck exercise under acute hypoxic exposure but does not impact cognitive performance.

Acute ingestion of exogenous ketone supplements in the form of a (R)-3-hydroxybutyl (R)-3-hydroxybutyrate (R-BD R-βHB) ketone monoester (KME) can attenuate declines in oxygen availability during hypoxic exposure and might impact cognitive performance at rest and in response to moderate-intensity exercise. In a single-blind randomized crossover design, 16 males performed assessments of cognitive performance before and during hypoxic exposure with moderate exercise [2 × 20 min weighted ruck (∼22 kg) at 3.2 km/h at 10% incline] in a normobaric altitude chamber (4572 m, 11.