Environmental factors and microbial interactions drive microbial community succession during solid-state fermentation of corn husk for microbial biomass protein production.

Corn husk, a predominant byproduct derived from intensive corn processing, is characterized by high cellulose content, low protein content, and poor palatability, which makes it difficult to be fully utilized by ruminants. This investigation employed corn husk as substrate for microbial protein production through a two-stage open solid-state fermentation (SSF) system using and yeast strains. The fermentation process yielded a 65.

Fabrication of imine-based COFs with different linker lengths for HRP and GOx immobilization and colorimetric detection of glucose and HO.

Covalent organic frameworks (COFs) have emerged as excellent candidates for enzyme immobilization due to their high surface tunability, diverse structures, inherent porosity, and metal-free characteristics. However, reports on optimizing the efficiency of immobilized enzymes in COFs by controlling linker length are scarce. In this work, horseradish peroxidase (HRP) and glucose oxidase (GOx), are co-immobilized on three imine-based COFs with different linker lengths using a one-pot method, resulting in HRP&GOX@COF (COF = LZU1, TbBD, TbDI).

Engineering Exopolysaccharide Biosynthesis of to Promote Electroactive Biofilm Formation for Liquor Wastewater Treatment.

Microbial electrochemical systems (MESs), as a green and sustainable technology, can decompose organics in wastewater to recover bioelectricity. Electroactive biofilms, a microbial community structure encased in a self-produced matrix, play a decisive role in determining the efficiency of MESs. However, as an essential component of the biofilm matrix, the role of exopolysaccharides in electroactive biofilm formation and their influence on extracellular electron transfer (EET) have been rarely studied.

Impact of a random TN5 mutation on endoglucanase secretion in ruminal cellulolytic Escherichia coli.

Objective: Most protein secretion systems are found in gram-negative bacteria, but the mechanism of endoglucanase (BcsZ) secretion in Escherichia coli (E. coli) remains unclear.

Methods: In this study, we used JBZ-DH5α (which overexpresses BcsZ on the E.

Engineered Cell Elongation Promotes Extracellular Electron Transfer of Shewanella Oneidensis.

To investigate how cell elongation impacts extracellular electron transfer (EET) of electroactive microorganisms (EAMs), the division of model EAM Shewanella oneidensis (S. oneidensis) MR-1 is engineered by reducing the formation of cell divisome. Specially, by blocking the translation of division proteins via anti-sense RNAs or expressing division inhibitors, the cellular length and output power density are all increased.

Engineering Shewanella oneidensis-Carbon Felt Biohybrid Electrode Decorated with Bacterial Cellulose Aerogel-Electropolymerized Anthraquinone to Boost Energy and Chemicals Production.

Interfacial electron transfer between electroactive microorganisms (EAMs) and electrodes underlies a wide range of bio-electrochemical systems with diverse applications. However, the electron transfer rate at the biotic-electrode interface remains low due to high transmembrane and cell-electrode interfacial electron transfer resistance. Herein, a modular engineering strategy is adopted to construct a Shewanella oneidensis-carbon felt biohybrid electrode decorated with bacterial cellulose aerogel-electropolymerized anthraquinone to boost cell-electrode interfacial electron transfer.

Overexpression of enzymes in glycolysis and energy metabolic pathways to enhance coenzyme Q10 production in VK-2-3.

We subjected the components of the glycolysis and energy metabolism pathways of (. ) to metabolic engineering to improve the titer and yield of coenzyme Q10 (CoQ10). Phosphofructokinase (PFK), cyclic adenylate-dependent protein kinase (PKAC), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and adenosine triphosphate hydrolase (KdpC) were overexpressed in .

Isolation and Screening of High-Yielding α-Amylase Mutants of Bacillus subtilis by Heavy Ion Mutagenesis.

To improve fermentative production of α-amylase, heavy-ion mutagenesis technology was used to irradiate Bacillus subtilis (B. subtilis) to obtain the high yielding mutants in this study. After continuous cultivation for 12 generations, eight mutants exhibited positive mutation rate with greater H/C.