Proline-adjacent phosphosites on S. cerevisiae histone demethylase Rph1p are salt stress responsive and important for cell growth under salt stress.

Phosphorylation of histone lysine demethylases is an important mechanism by which the cell modulates chromatin dynamics to regulate its response to stress. There is evidence that the Saccharomyces cerevisiae H3K36me2/3 demethylase, Rph1p, is an integrator of many signalling events. However, the regulatory function of most Rph1p phosphosites in stress response pathways remains unknown.

Impact of Gamma-Secretase Inhibition on Outcomes Following BCMA CAR-T Therapy in Multiple Myeloma: A Comparison of Two Phase 1 Trials.

Background: BCMA-directed chimeric antigen receptor (CAR)-T cell therapy represents a major therapeutic breakthrough for relapsed/refractory multiple myeloma (RRMM), offering deep and durable responses in heavily pretreated patients. However, a subset of patients experience early relapse or fail to respond, highlighting the need for strategies to enhance efficacy. Gamma-secretase inhibitors (GSIs) have been shown to increase surface BCMA expression on malignant plasma cells and may potentiate the activity of BCMA CAR-T cells, particularly in patients with low baseline BCMA antigen density.

Real-world clinical data on the long-term effectiveness and safety of generic racemic ketamine treatment.

Ketamine is a highly effective treatment for difficult-to-treat or treatment-resistant depression (TRD). The commercially developed intranasal spray containing S-ketamine has demonstrated short and long-term efficacy and safety in phase 3 clinical trials leading to regulatory approval for TRD in many countries. Conversely, generic racemic ketamine is prescribed 'off-label'.

Screening for depression in new fathers beyond the clinic: An observational study.

Background: Perinatal depression among fathers can impact their own wellbeing, as well as that of their partner and child. Infrastructure to support universal paternal mental health screening is limited. Digital screening embedded within the SMS4dads text messaging program provides an opportunity to reach and refer distressed fathers.

Aseptic technique in clinical nursing settings: a scoping review.

Introduction: Aseptic technique forms a component of Standard Precautions and is a foundational concept in nursing practice. The application of that standard appears to vary between context and nurse. The variation of principles and application between facilities and individuals may reflect the current disparity in evidence-based literature.

A potent NLRP3 inhibitor effective against both MCC950-sensitive and -resistant inflammation.

The nucleotide-binding oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome detects a broad spectrum of pathogen- and damage-associated molecular patterns (PAMPs and DAMPs), initiating inflammatory responses through caspase-1 activation and interleukin (IL)-1β/IL-18 release. Dysregulated NLRP3 activation is implicated in a range of diseases, including infectious diseases, autoinflammatory disorders, metabolic disorders, and cancer, making it an attractive therapeutic target. Here, we identify ZAP-180013 as a potent and selective small-molecule inhibitor of NLRP3 through high-throughput chemical screening.

Sex Differences in the Joint Trajectories of Depressive Symptoms and Body Mass Index From Adolescence to Early Adulthood: Longitudinal Observational Study.

Background: Adolescence is a critical transitional period between childhood and adulthood, marked by dramatic changes in physical and psychosocial health. Adolescents are vulnerable to both depression and adiposity, but how these conditions evolve over time from adolescence to early adulthood and whether sex differences exist remains unclear.

Objective: This study aims to first identify the population heterogeneity in the joint trajectories of depressive symptoms and BMI from adolescence to early adulthood and then explore the sex differences in the joint trajectories.

Interpretable machine learning model for characterizing magnetic susceptibility-based biomarkers in first episode psychosis.

Background And Purpose: Several studies have shown changes in neurochemicals within the deep-brain nuclei of patients with psychosis. These alterations indicate a dysfunction in dopamine within subcortical regions affected by fluctuations in iron concentrations. Quantitative Susceptibility Mapping (QSM) is a method employed to measure iron concentration, offering a potential means to identify dopamine dysfunction in these subcortical areas.

Effectiveness of cannabinoids on subjective sleep quality in people with and without insomnia or poor sleep: A systematic review and meta-analysis of randomised studies.

Study Objectives: This systematic review and meta-analysis assessed the efficacy of cannabinoids compared to placebo for improving sleep quality.

Methods: Searches were conducted in MEDLINE, Embase, and Cochrane databases for randomised controlled trials comparing cannabinoids vs. placebo for improving sleep quality in adults with or without insomnia or poor sleep.

Autism and the Oral Microbiome: A Systematic Review of Host-microbial Interactions and Diversity.

Background: Emerging evidence suggests a link between the oral microbiome and autism spectrum disorder (ASD), a neurodevelopmental condition characterised by social and behavioural impairments. The vast microbial reservoirs in the gut complement those of the oral cavity, suggesting a potential oral-gut-brain axis that may influence ASD and perhaps other neurological diseases, such as Parkinson's syndrome and Alzheimer's disease. For the first time, this systematic review synthesises the current knowledge of oral microbiome composition, diversity, and functionality in ASD and its potential diagnostic and therapeutic implications.

Chemical forensic profiling of compounds related to the chemical weapons convention using 2D and 3D diffusion-ordered NMR spectroscopy.

The ongoing use of chemical warfare agents (CWAs) in conflicts, assassinations, and terrorist attacks means that the detection and identification of these compounds are crucial. The forensic identification of organophosphorus nerve agents (OPNAs) and their precursors and degradation products remains challenging due to the destructive nature and extensive preparation required for conventional chromatographic methods. In this study, we characterise precursor and degradation products of Novichok analogues, including 1,1,3,3-tetramethylguanidine, N,N-diethylpentanimidamide, N,N-dipropylbutanimidamide, using 2D ¹H-¹³C heteronuclear multiple quantum coherence (HMQC) NMR.

'More prioritisation on food security' - exploring what initiative leaders envision for a food secure regional Australia.

Food security is a particular challenge in rural, regional and remote locations due to complexities associated with food availability, access, utilisation, stability, agency and sustainability dimensions. Existing approaches, such as emergency food relief, have been found to inadequately address these challenges, suggesting that a comprehensive portfolio of solutions could be more effective. Understanding what food security initiative leaders operating in these areas consider important will help establish a shared goal to guide the development of a portfolio approach.

Reduced anxiety and depression predict improved health-related quality of life in school-based CBT.

Health-related quality of life (HRQOL) adds important information about the quality and usefulness of interventions. It has not previously been examined in school-based indicated prevention for anxiety in adolescents. Using secondary analyses of data from a randomized controlled trial of cognitive behavioral therapy (CBT) interventions for anxious adolescents (N = 302; mean age 14), we examined whether HRQOL increased during and after treatment.

Automated detection of pediatric congenital heart disease from phonocardiograms using deep and handcrafted feature fusion.

Congenital heart disease (CHD) is the most common type of birth defect, impacting about 1% of live births worldwide. Echocardiography, the gold-standard diagnostic method, is costly and inaccessible in low-resource settings. Diagnosis is delayed due to limited skilled experts, whose ability to interpret pathological patterns varies significantly, causing inter- and intra-clinician variability.

How is power reflected in the patient centred care literature? A critical review of power in the centredness literature through a social science lens.

Patient-centredness is a key component of high-quality healthcare. A critical part of centredness is the sharing of power within the practitioner-patient relationship. Yet, there has been little reflection on how power, shared power and empowerment are reflected in healthcare literature about centredness.

Therapists' Role in Patient Adherence to Internet-Based Cognitive Behavioral Therapy: Qualitative Study.

Background: Internet-based cognitive behavioral therapies (iCBTs) are typically categorized into 2 types: therapist-assisted and self-guided. Both formats have accumulated substantial evidence supporting their cost-effectiveness and efficacy in treating a range of mental health conditions. However, therapist-assisted iCBTs tend to show lower dropout rates than self-guided versions.

Health Care Professionals' Experiences Regarding Facilitators of and Barriers to Sustained Use of Social Robot Ivy for People With Intellectual Disabilities: Qualitative Interview Study.

Background: Labor shortages in health care pose significant challenges to sustaining high-quality care for people with intellectual disabilities. Social robots show promise in supporting both people with intellectual disabilities and their health care professionals; yet, few are fully developed and embedded in productive care environments. Implementation of such technologies is inherently complex, requiring careful examination of facilitators and barriers influencing sustained use.

eHealth and Hypertensive Disorders of Pregnancy: Systematic Review.

Background: Hypertensive disorders of pregnancy (HDP) affect up to 10% of pregnancies and can have adverse short and long-term implications for women and their babies. eHealth interventions include any health service or treatment delivered using the internet and related technology that aims to facilitate, capture, or exchange knowledge. eHealth interventions are increasingly used across many health care settings with improved outcomes.

Community Connectedness as a Source of Adherence to HIV Prevention Behaviors and Resilience Among Transgender Women of Color in New York City, 2020-2022.

To examine how one's community connectedness may act as a source of resilience and promote HIV prevention and care behaviors among transgender women of color. We analyzed survey data from 313 transgender women of color living in New York City collected from August 2020 to November 2022. The Community Connectedness Scale asks participants about their baseline feelings of connection, feelings of inclusion, feelings of belonging, feelings of isolation, and feelings of being unlike in relation to the transgender community.

Evaluating the completeness of postoperative endoscopic recurrence assessment in Crohn's disease patients with Kono-S anastomoses.

Background And Aims: Despite therapeutic advances, resection rates in Crohn's disease remain high. Kono-S is a novel anastomosis for ileocolonic resections; however, its altered configuration may challenge standard endoscopic assessment, particularly in the absence of validated scoring tools. This study evaluated the endoscopic assessment of Kono-S anastomosis anatomy and recurrence stratification using Rutgeert's score.

Influences on Food Choices of Cambodian Women for Themselves and Their Families.

BackgroundThe nutritional status of Cambodian women and children remain poor despite implementation of dietary intake interventions. Cambodia-specific studies have explored how education influences nutrition and health behavior, but not factors influencing Cambodian women's food decision-making for themselves and their families.ObjectiveTo understand Cambodian women's food decision-making, particularly related to barriers and enablers of healthy eating for themselves and their families.

Mechanotransduction mechanisms in human erythrocytes: Fundamental physiology and clinical significance.

The hallmarks of mechanosensitive ion channels have been observed for half a century in various cell lines, although their mechanisms and molecular identities remained unknown until recently. Identification of the bona fide mammalian mechanosensory Piezo channels resulted in an explosion of research exploring the translation of mechanical cues into biochemical signals and dynamic cell morphology responses. One of the Piezo isoforms - Piezo1 - is integral in the erythrocyte (red blood cell; RBC) membrane.

Chronic Cough Hypersensitivity as a Neuropathic Disorder: Implications for Management and New Treatments.

Chronic cough can coexist with or without pulmonary and extrapulmonary conditions and can be refractory to therapies that improve these associated conditions. It is underlined by cough hypersensitivity, which is characterized by increased cough responses to stimuli that affect the airways and vagally innervated tissues as well as by excessive cough responses to innocuous stimuli, and it is caused by neuroinflammatory and neuropathic mechanisms at both peripheral and central levels. The management of chronic cough starts with exclusion of associated conditions, followed by use of neuromodulators and speech and language therapy.

The PROtective VEntilation (PROVE) Network - advancing research and collaboration in mechanical ventilation.

The PROtective VEntilation (PROVE) Network is a globally-recognized collaborative research group dedicated to advancing research, education, and collaboration in the field of mechanical ventilation. Established to address critical questions in intraoperative and intensive care ventilation, the network focuses on improving outcomes for patients undergoing mechanical ventilation in diverse settings, including operating rooms, intensive care units, burn units, and resource-limited environments in low- and middle-income countries. The PROVE Network is committed to generating high-quality evidence through a comprehensive portfolio of investigations, including randomized clinical trials, observational research, and meta-analyses.