Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Mustard agents, including sulphur mustard (SM) and nitrogen mustard (NM), are chemical warfare agents that can cause blistering of the skin and mucous membranes upon contact. Although SM and NM both have dermal effects, their medical management of systemic poisoning differs significantly. A rapid and simple method for detecting and discriminating between SM and NM would be greatly valuable. We report several fluorescent sensors for the detection and differentiation of SM and NM. Among these sensors, fluorescein SN-R2-2 exhibits dual fluorescence responses: quenching with NM and enhancement with SM under 430 nm excitation, achieving rapid detection (40 s for SM and 6 min for NM) and high sensitivity (limit of detection = 1.43 μM for SM and 18.01 μM for NM). Its solvent-independent performance further provides field applicability. SN-R2-2 effectively visualised different concentrations of SM and NM in living mice. SN-R2-2 holds promise for military and public safety emergencies, enhancing chemical incident response and medical triage. To the best of our knowledge, SN-R2-2 is the first sensor for SM and NM discrimination.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/d5an00673b | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel fluorescent aptasensor using magnetic silica nanospheres and Ag@MOF nanocomposites for extraction and detection of Salmonella Typhimurium in eggs.
Mikrochim Acta
September 2025
Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Salmonella Typhimurium (S. Typhimurium) is one of the most common food-borne diseases, highlighted as the top food-borne bacterial pathogen in the world with a low infectious dose (1 CFU) and high mortality rate. It is commonly associated with numerous foods such as dairy products, protein sources (multiple types of meat, poultry, and eggs), and bakery products.
View Article and Find Full Text PDFDevelopment of smartphone-based AIE fluorescence-quenching immunochromatographic sensors for the detection of illicit drugs in various complex sample matrices.
Anal Bioanal Chem
September 2025
GuangDong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Illicit drug abuse poses a significant global threat to public health and social security, highlighting the urgent need for rapid, sensitive, and versatile detection technologies. To address the limitations of traditional chromatographic techniques-such as high costs and slow response times-and the drawbacks of conventional immunochromatographic sensors (ICS), including low sensitivity and non-intuitive signal outputs, a fluorescence-quenching ICS (FQICS) was developed. This sensor leverages fluorescence resonance energy transfer (FRET) between aggregation-induced emission fluorescent microspheres (AIEFMs) and gold nanoparticles (AuNPs).
View Article and Find Full Text PDFVisualizing intracellular glycine with two-dye and single-dye ratiometric RNA-based sensors.
Nucleic Acids Res
September 2025
Department of Chemistry and Henry Eyring Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, United States.
Glycine is an important metabolite and cell signal in diverse organisms, yet tools to visualize intracellular glycine dynamics have not been developed. In this study, diverse and bright RNA-based glycine biosensors were developed by fusing the architecturally complex glycine riboswitch with Broccoli class fluorogenic aptamers. The brightest sensor with the highest activation, glyS, and its two-dye ratiometric counterpart, Pepper-glyS, allowed for visualization of a drug-induced accumulation of endogenous glycine in live Escherichia colicells.
View Article and Find Full Text PDFMichaelis-Menten kinetics of RasGAP proteins by a rapid fluorescence-based assay.
Methods
September 2025
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA; Department of Pharmacology, Yale University, New Haven, CT 06510, USA; Yale Cancer Center, Yale University, New Haven, CT 06510, USA. Electronic address:
Ras small GTPases are essential for a wide range of cellular processes. These proteins cycle between the GDP-loaded and GTP-loaded states, and the actions of GTPase activating proteins (GAPs) are necessary to stimulate Ras-mediated GTP hydrolysis. Here, we provide a protocol to achieve Michaelis-Menten kinetic profiling of GAP-mediated stimulation of a small GTPase by real-time monitoring of inorganic phosphate release in vitro.
View Article and Find Full Text PDFLong-wavelength emission carbon dots as ratiometric fluorescent and colorimetric dual-mode sensors for environmental sensing and bioimaging of hypochlorite.
J Hazard Mater
August 2025
Institute of Environmental Science, School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China. Electronic address:
For the first time, long-wavelength red emission carbon dots (R-CDs) were prepared as ratiometric fluorescent and colorimetric dual-mode sensors for detecting ClO using a simple one-step hydrothermal method. R-CDs exhibited intrinsic red fluorescence at 587 nm. Upon interaction with ClO, a new and enhanced green fluorescence at 535 nm was observed, which was attributed to resulting from the oxidation of surface hydroxyl (-OH) groups to carbonyl (CO) groups.
View Article and Find Full Text PDF