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This study investigated the impact of cooking methods (boiling, roasting, and frying) on the flavor of Chuanbai Rex and Hyla rabbit by employing gas chromatography-ion mobility spectrometry (GC-IMS) and widely targeted metabolomics analysis to compare the differences in volatile organic compounds (VOCs) and metabolites. GC-IMS identified 45 VOCs, primarily aldehydes. Frying reduced the levels of aldehydes and esters, whereas roasting accentuated VOCs differences between the two breeds. Multivariate statistical analysis identified 14 key VOCs that distinguished the processed meat samples. Metabolomics identified 1118 metabolites, with amino acids and its derivatives (32.38 %), organic acids (12.08 %), fatty acyls (11.72 %), and glycerophospholipids (8.94 %) as the main components. Among them, 184 differential metabolites were mainly associated with amino acid and lipid metabolism. KEGG enrichment analysis revealed that the differential metabolites were mainly enriched in pathways such as ABC transporters and glycerophospholipid metabolism. These findings provide scientific insights for optimizing rabbit meat quality.
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http://dx.doi.org/10.1016/j.foodchem.2025.145782 | DOI Listing |
J Agric Food Chem
September 2025
College of Basic Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi 030000, China.
Atherosclerosis (AS) is increasingly recognized as a disease influenced not only by lipid metabolism and inflammation but also by the gut microbiota and their bioactive metabolites. Isoquercitrin (ISO), a natural flavonoid with food-medicine homology, has shown promising antiatherosclerotic potential, yet its underlying mechanisms remain unclear. In this study, ISO administration significantly reduced plaque burden, improved lipid profiles, and restored gut microbial balance by enriching beneficial taxa, such as , , and .
View Article and Find Full Text PDFInsect Sci
September 2025
State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, China.
The ectoparasitic honeybee (Apis mellifera) mite Tropilaelaps mercedesae represents a serious threat to Asian apiculture and a growing concern for global beekeeping due to its high reproductive capacity and host adaptability. However, the regulatory mechanisms underlying its host adaptation across life stages remain poorly characterized. Here, we performed integrated transcriptomic, proteomic, and metabolomic analyses of female mites at 4 key postembryonic developmental stages: protonymphs, deutonymphs, mature adults, and reproductive adults.
View Article and Find Full Text PDFParasite Immunol
September 2025
Department of Parasitology, Leiden University Medical Center, Leiden, the Netherlands.
Schistosome parasites are known to modulate host immune responses, which is achieved in part through the release of excretory/secretory (ES) products, including extracellular vesicles (EVs). During chronic schistosomiasis, increased regulatory responses are found, which include enhanced IL-10 production by B (Breg) cells. ES products from schistosome eggs are able to induce IL-10 production by B cells.
View Article and Find Full Text PDFPhytomedicine
August 2025
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:
Background: Traditional Chinese medicines (TCMs) have a long-standing history and diverse applications. However, their complex multi-component compositions and intricate mechanisms of action pose significant challenges for modern scientific investigation. Addressing these complexities requires advanced techniques capable of dissecting cellular and molecular interactions with high resolution.
View Article and Find Full Text PDFHelicobacter
September 2025
Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Background: Several clinical studies have demonstrated that Helicobacter pylori (Hp) infection may exacerbate the progression of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD); however, the underlying mechanisms remain unclear. This study aims to investigate the characterization of the gastric microbiome and metabolome in relation to the progression of MASLD induced by Hp infection.
Methods: We established a high-fat diet (HFD) obese mouse model, both with and without Hp infection, to compare alterations in serum and liver metabolic phenotypes.