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Cotton leaf diseases significantly impact global cotton yield and quality, threatening the livelihoods of millions of farmers. Traditional diagnostic methods are often slow, subjective, and unsuitable for large-scale agricultural monitoring. This study proposes an interpretable and efficient deep learning (DL) framework for the accurate classification of cotton leaf diseases using a hybrid architecture that combines EfficientNetB3 and InceptionResNetV2. The system demonstrates excellent performance, achieving 98.0% accuracy, 98.1% precision, 97.9% recall, an F1-score of 98.0%, and an AUC-ROC of 0.9992. Minimal overfitting was observed, with low training and validation losses and high per-class performance, even in visually similar disease cases such as bacterial blight and target spot. In addition to strong predictive accuracy, the framework incorporates explainable AI (XAI) techniques, including LIME and SHAP, to enhance model transparency. These tools highlight the key visual features used in predictions, providing valuable insights for agronomists and improving trust in AI-based systems. The model is lightweight and scalable, making it deployable on mobile or edge devices for real-time field applications. Overall, this research demonstrates the potential of combining transfer learning and XAI to develop reliable, interpretable, and field-ready diagnostic tools for precision agriculture.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283425 | PMC |
http://dx.doi.org/10.1002/fsn3.70658 | DOI Listing |
Funct Integr Genomics
September 2025
Zhengzhou Research Base, State Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Zhengzhou University/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Zhengzhou, China.
In this study, a comprehensive genome-wide identification and analysis of the aldo-keto reductase (AKR) gene family was performed to explore the role of Gossypium hirsutumAKR40 under salt stress in cotton. A total of 249 AKR genes were identified with uneven distribution on the chromosomes in four cotton species. The diversity and evolutionary relationship of the cotton AKR gene family was identified using physio-chemical analysis, phylogenetic tree construction, conserved motif analysis, chromosomal localization, prediction of cis-acting elements, and calculation of evolutionary selection pressure under 300 mM NaCl stress.
View Article and Find Full Text PDFBiology (Basel)
August 2025
State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Natural enemies commonly probe larval bodies and frass with their antennae for prey hunting. However, the attractants to natural enemies emitted directly from hosts and host-associated tissues remained largely unknown. Here, we used two generalist noctuid species, (Hübner) and (JE Smith), along with the larval endoparasitoid (Haliday) to address the question.
View Article and Find Full Text PDFBMC Plant Biol
September 2025
Nuclear Institute for Agriculture and Biology College (NIAB-C), Faisalabad, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, 45650, Pakistan.
Background: Escalating global temperatures pose an ongoing threat to cotton production by disrupting essential morphological, physiological, and metabolic processes during early plant development. These early stages are critical for crop establishment, yet the genetic basis of heat tolerance at this phase remains insufficiently characterized. Therefore, advancing our understanding of early-stage responses is essential for the development of heat-tolerant genotypes.
View Article and Find Full Text PDFPlants (Basel)
August 2025
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Organisms, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China.
RNA polymerase II (Pol II) has been shown to participate in various biological processes in plants, but its function in response to abiotic stress in cotton remains unclear. This study aimed to elucidate the role of the third-largest subunit of Pol II (NRPB3) in the response of cotton to drought and salt stress through molecular biology and physiological methods. Real-time fluorescence quantitative PCR was used to analyze the expression pattern of in roots, stems, leaves, and cotyledons and to detect changes in its expression under drought, NaCl, and ABA treatments.
View Article and Find Full Text PDFPlants (Basel)
August 2025
College of Environment and Resources, College of Carbon Neutrality, Zhejiang A&F University, Hangzhou 311300, China.
Hemp stalk, a widely available agricultural waste, is an ideal eco-friendly raw material for biochar production. Carbonization experiments were conducted as a novel approach for the scalable and value-added utilization of hemp stalk under oxygen-exclusion conditions. The effects of feedstock types- (KS), spp.
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