Negatively Regulates Drought and Salt Tolerance in Cotton.

RNA polymerase II (Pol II) has been shown to participate in various biological processes in plants, but its function in response to abiotic stress in cotton remains unclear. This study aimed to elucidate the role of the third-largest subunit of Pol II (NRPB3) in the response of cotton to drought and salt stress through molecular biology and physiological methods. Real-time fluorescence quantitative PCR was used to analyze the expression pattern of in roots, stems, leaves, and cotyledons and to detect changes in its expression under drought, NaCl, and ABA treatments.

Identification of the full-length GbERD7 gene family in Gossypium barbadense and functional analysis of the role of the GbERD7g gene in drought and salt tolerance.

ERD (early response to dehydration) genes are promptly upregulated under dehydration stress and are pivotal in plant development. Nonetheless, the precise impact of the ERD7 gene on the response of cotton to abiotic stress remains unclear. The physical and chemical characteristics, gene architecture, gene collinearity, and transcriptomic profiles were examined.

The Gma-miR4359b/GmFBX193 module is involved in the response of soybean to salt stress.

MicroRNAs (miRNAs) are key regulators of F-box-mediated signal transduction in plants and play important roles in the stress response. The ways in which miRNAs target F-box genes in soybeans under salt stress are currently unknown. In this study, the functional role of GmFBX193, the gene target of gma-miR4359b, under salt stress was investigated.

The gma-miR164a/GmNAC115 module participates in the adaptation of soybean to drought and salt stress by influencing reactive oxygen species scavenging.

The regulatory modules formed by microRNAs and transcription factors (TFs) play important roles in plant stress responses and adaptations. However, the function and molecular mechanism of the gma-miR164a/GmNAC115 module in soybean responses to drought and salt stress remain unclear. This study revealed that the soybean gma-miR164a/GmNAC115 module is involved in the response to abiotic stress.

Identification and Analysis of the Plasma Membrane H-ATPase Gene Family in Cotton and Its Roles in Response to Salt Stress.

Plant plasma membrane (PM) H-ATPase functions as a proton-motive force by exporting cellular protons to establish a transmembrane chemical gradient of H ions and an accompanying electrical gradient. These gradients are crucial for plant growth and development and for plant responses to abiotic and biotic stresses. In this study, a comprehensive identification of the PM H-ATPase gene family was conducted across four cotton species.

Genome-Wide Identification of the Gene Family in Sea-Island Cotton () and the Active Regulation of Drought Resistance in Cotton by .

Cotton is an economically critical crop worldwide, and drought stress strongly affects its growth and development. Ubiquitination modifies protein activity and is crucial in numerous biological processes. Ubiquitin-conjugating enzymes serve as intermediaries in the protein ubiquitination process and play important roles in plant responses to abiotic stress.

Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton.

The SEVEN IN ABSENTIA (SINA) E3 ubiquitin ligase is widely involved in drought and salt stress in plants. However, the biological function of the SINA proteins in cotton is still unknown. This study aimed to reveal the function of through biochemical, genetic and molecular approaches.

The Gma-miR394a/GmFBX176 module is involved in regulating the soybean (Glycine max L.) response to drought stress.

Drought seriously affects the yield and quality of soybean. Previous studies have shown that the gma-miR394a/GmFBX176 module regulates the response of Arabidopsis to drought stress. However, whether the gma-miR394a/GmFBX176 module is involved in the regulation of the soybean drought stress response remains unclear.

The sea-island cotton GbTCP4 transcription factor positively regulates drought and salt stress responses.

TCP transcription factors play important regulatory roles in plant growth and development; however, their function in response to salt and drought stress in sea-island cotton (Gossypium barbadense) is unknown. Here, GbTCP4 expression was induced by abscisic acid (ABA), drought, and NaCl treatments. Under drought stress, compared to wild-type (WT) Arabidopsis, transgenic GbTCP4-overexpressing Arabidopsis showed increased seed germination rate, root length and survival rate; additionally, it was ABA-insensitive at the germination stage but ABA-sensitive at the seedling stage, showing reduced stomatal opening and ABA enrichment.

Heterologous overexpression of the GbTCP5 gene increased root hair length, root hair and stem trichome density, and lignin content in transgenic Arabidopsis.

Teosinte branched1/cycloidea/proliferating cell factor1 (TCP) is a plant-specific protein family member involved in plant growth and development. However, the functions of most members of the cotton TCP family are unknown. In this study, the GbTCP5 gene encodes a sea-island cotton class II TCP CIN subclass transcription factor.

Stability of copper acetate at high P-T and the role of organic acids and CO in metallic mineralization.

Many metal deposits were formed by carbonic fluids (rich in CO) as indicated by fluid inclusions in minerals, but the precise role of CO in metal mineralization remains unclear. The main components in fluid inclusions, i.e.

Genome-wide association reveals genetic variation of lint yield components under salty field conditions in cotton (Gossypium hirsutum L.).

Background: Salinity is one of the most significant environmental factors limiting the productivity of cotton. However, the key genetic components responsible for the reduction in cotton yield in saline-alkali soils are still unclear.

Results: Here, we evaluated three main components of lint yield, single boll weight (SBW), lint percentage (LP) and boll number per plant (BNPP), across 316 G.

The Chalcone Isomerase Family in Cotton: Whole-Genome Bioinformatic and Expression Analyses of the L. Response to Wilt Infection.

Chalcone isomerase (CHI) is a key component of phenylalanine metabolism that can produce a variety of flavonoids. However, little information and no systematic analysis of genes is available for cotton. Here, we identified 33 genes in the complete genome sequences of four cotton species ( L.

Heterologous Expression of GbTCP4, a Class II TCP Transcription Factor, Regulates Trichome Formation and Root Hair Development in Arabidopsis.

Two class I family teosinte branched1/cycloidea/proliferating cell factor1 (TCP) proteins from allotetraploid cotton are involved in cotton fiber cell differentiation and elongation and root hair development. However, the biological function of most class II TCP proteins is unclear. This study sought to reveal the characteristics and functions of the sea-island cotton class II TCP gene by biochemical, genetic, and molecular biology methods.

Overexpression of soybean miR169c confers increased drought stress sensitivity in transgenic Arabidopsis thaliana.

The miR169 family, a large-scale microRNA gene family conserved in plants, is involved in stress responses, although how soybean miR169 functions in response to drought stress remains unclear. We show that gma-miR169c exerts a negative regulatory role in the response to drought stress by inhibiting the expression of its target gene, nuclear factor Y-A (NF-YA). A real-time RT-PCR analysis indicated that gma-miR169c is widely expressed in soybean tissues and induced by polyethylene glycol (PEG), high salt, cold stress and abscisic acid (ABA).

Overexpression of TaSIM provides increased drought stress tolerance in transgenic Arabidopsis.

Drought is the most serious meteorological disaster affecting wheat production. Members of the R2R3-MYB gene subfamily play a crucial role in the regulation of the wheat drought stress response. In this study, the function of polyethylene glycol (PEG)-induced expression of the wheat R2R3-MYB gene TaSIM in response to drought stress was characterized.

[Electroacupuncture Intervention Improves Cartilage Degeneration and Subchondral Bone Osteoporosis of Knee-joint Possibly by Adjusting OPG/RANK/RANKL Signaling in Ovariectomized Rats].

Objective: To observe the effect of electroacupuncture (EA) on histopathological changes of cartilage and subchondral bone and osteoprotegerin (OPG)，receptor activator of nuclear factor-κB (RANK), and RANK ligand (RANKL) (OPG/RANK/RANKL) signaling and the expression of matrix metalloproteinase-13 (MMP-13) in ovariectomized(OVX)rats, so as to explore its mechanism underlying improvement of osteoporosis.

Methods: Three-month female SD rats were randomly divided into sham operation, model and EA groups (＝8 in each group). The ovoariectomy model was established by resection of bilateral ovaries.

The Gene Encoding Subunit A of the Vacuolar H-ATPase From Cotton Plays an Important Role in Conferring Tolerance to Water Deficit.

In plant cells, vacuolar H-ATPases (V-ATPases) are responsible for deacidification of the cytosol and energisation of the secondary transport processes across the tonoplast. A number of V-ATPase subunit genes have been demonstrated to be involved in the regulation of the plant response to water deficit. However, there are no reports on the role of V-ATPase subunit A (VHA-A) in dehydration tolerance of cotton.

A new genetic mechanism of natural gas accumulation.

Natural gas of organic origin is primarily biogenic or thermogenic; however, the formation of natural gas is occasionally attributed to hydrothermal activity. The Precambrian dolomite reservoir of the Anyue gas field is divided into three stages. Dolomite-quartz veins were precipitated after two earlier stages of dolomite deposition.

An in-situ Raman study on pristane at high pressure and ambient temperature.

The CH Raman spectroscopic band (2800-3000cm) of pristane was measured in a diamond anvil cell at 1.1-1532MPa and ambient temperature. Three models are used for the peak-fitting of this CH Raman band, and the linear correlations between pressure and corresponding peak positions are calculated as well.

The wheat salinity-induced R2R3-MYB transcription factor TaSIM confers salt stress tolerance in Arabidopsis thaliana.

MYB transcription factors are a large family of proteins involved in plant development and responses to stress. In this study, the wheat salinity-induced R2R3-MYB transcription factor TaSIM was functionally characterized, with a focus on its role in salt stress tolerance. TaSIM protein enters the nucleus and binds to the MYB-binding site II motif.

Proteomic responses of drought-tolerant and drought-sensitive cotton varieties to drought stress.

Drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. Although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. To determine the effects of drought stress on cotton seedlings, we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry to comparatively analyze proteome of drought-responsive proteins during the seedling stage in two cotton (Gossypium hirsutum L.

Prediction and identification of natural antisense transcripts and their small RNAs in soybean (Glycine max).

Background: Natural antisense transcripts (NATs) are a class of RNAs that contain a sequence complementary to other transcripts. NATs occur widely in eukaryotes and play critical roles in post-transcriptional regulation. Soybean NAT sequences are predicted in the PlantNATsDB, but detailed analyses of these NATs remain to be performed.