Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: MicroRNAs (miRNAs), a type of small non-coding RNA sequences, are very important biomarkers and are involved in various physiological processes, such as cell proliferation, growth, differentiation, and apoptosis. Many reports have shown that miRNAs are closely associated with a variety of diseases, including neurodegenerative diseases and cancer. Currently, researchers have developed various methods for miRNAs detection, such as fluorescence, surface-enhanced Raman scattering, electrochemiluminescence, and electrochemical sensing. The detection of multiple miRNAs is significant for the diagnosis of diseases. However, it is rare for a single biosensing system to ultra-sensitively detect multiple miRNAs.
Results: Based on enzyme biofuel cells (EBFCs) and catalytic hairpin assembly (CHA), a novel self-powered biosensor is designed for the sequential ultra-sensitive detection of miRNA-21, miRNA-146a, miRNA-155. First, miRNA-21 initiates CHA, and hairpin-tetrahedral DNA nanostructure (H-TDN) is captured on the biocathode. As a result, the open-circuit voltage 1 (E) increases, i.e., a signal "on" state. Next, in the presence of miRNA-146a, another CHA process is activated, and DNA1-glucose oxidase (DNA1-GOD) is replaced by hairpin 2 (H2) on the bioanode. This causes the open-circuit voltage (E) to decrease, i.e., a signal "off" state. Finally, when miRNA-155 is present, DNA2-GOD is captured by miRNA-155. And E increases, i.e., a signal "on" state. Moreover, the self-powered biosensor possesses good selectivity, high reproducibility, and excellent stability for miRNAs assay.
Significance: Based on the variation of the open-circuit voltage, this novel self-powered biosensor exhibits ultra-highly sensitive detection for miRNA-21 with the limit of detection (LOD) of 0.36 fM, miRNA-146a with the LOD of 0.16 fM, and miRNA-155 with the LOD of 0.23 fM. This novel self-powered biosensor provides a feasible solution to explore ultra-sensitive sequential biosensors with multi-target detection.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2025.344346 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Mixed metal metal-organic framework synergized with functional liposomes boosts bipolar-driven organic photoelectrochemical transistor for highly sensitive biodetection.
Biosens Bioelectron
December 2025
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:
Organic photoelectrochemical transistor (OPECT) has emerged as a promising platform for investigating photoactive biomolecular interactions and advancing bioanalytical detection systems. However, many important challenges and hurdles remain implementing high gating effects and sensitive biosensing detection caused by the inherent limitations of the configuration of the photoelectrode structures and innovative biosensing. Inspired by the self-powered photoelectrochemical (PEC) systems and liposomes-assisted bioanalysis for signal amplification, a bipolar-driven poly(3,4-ethylenedioxythiophene): poly(styrene sulfonate) (PEDOT:PSS) OPECT based on CdS/Mixed Metal Metal-Organic Framework (MM-MOF) photoanode and a poly(1,4-diethynylbenzene) (pDEB) cathode is proposed, and exhibits a considerable current gain of ca.
View Article and Find Full Text PDFDual DNAzyme-MoS/GDY Catalytic Assembly Enables Smartphone-Based Multiplex Detection of Sugarcane Pokkah Boeng Pathogens at Sub-Femtomolar Levels.
Anal Chem
September 2025
Education Department of Guangxi Zhuang Autonomous Region, Laboratory of Optic-electric Chemo/Biosensing and Molecular Recognition, Engineering Research Center of Low-carbon and High-quality Utilization of Forest Biomass, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Lab
Rapid on-site detection of sugarcane pokkah boeng disease caused by Fusarium pathogens remains challenging due to the lack of portable platforms combining high sensitivity and multiplexing capability. Here, we present a self-powered biosensor integrating a dual DNAzyme-driven catalytic system with a MoS/graphdiyne (GDY) nanohybrid-modified biofuel cell (EBFC) for simultaneous detection of and . The key innovation lies in the windmill-shaped dual DNAzyme structure that enables Mn/Mg-dependent target recycling, synergistically coupled with the hybridization chain reaction (HCR) and triplex catalytic hairpin assembly (TCHA) for exponential signal amplification.
View Article and Find Full Text PDFNovel Self-Powered Biosensor Based on Highly Efficient Cosignal Accelerator and Multiple Signal Amplification Strategies toward Ultrasensitive miRNA-141 Assay.
Anal Chem
August 2025
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China.
A highly sensitive self-powered biosensor is designed based on gold-platinum nanorods (AuPt NRs) and the cascade reaction of catalytic hairpin assembly (CHA) and hybrid chain reaction (HCR) toward the miRNA-141 assay. As a cosignal accelerator, AuPt NRs enhance electrical conductivity between glucose oxidase (GOD) and a carbon paper (CP) electrode, thereby assisting in output signal enhancement. The cascade reaction of CHA-HCR is employed to efficiently amplify the detection signal and improve the sensitivity of the self-powered biosensor.
View Article and Find Full Text PDFSingle-Atom Iron Boosts Interfacial Oxygen Reduction for Self-Powered Photoelectrochemical Biosensing.
Anal Chem
August 2025
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
The sluggish interfacial reaction of the photocathode seriously hinders the application of self-powered photoelectrochemical (PEC) systems. In order to solve the above problem, iron single-atom catalysts (Fe SACs) were used to accelerate the oxygen reduction reaction (ORR) at the photocathode interface. It made dissolved oxygen and water circulate efficiently between the photoanode BiS and the photocathode CuO/CuSnS interface, inhibited photogenerated carrier recombination, and greatly enhanced the cathodic photocurrent.
View Article and Find Full Text PDFNext-generation smart wound dressings: AI integration, biosensors, and electrospun nanofibers for chronic wound therapy.
J Biomater Sci Polym Ed
August 2025
Centre for Research in Environment, Sustainability Advocacy and Climate Change (REACH), Directorate of Research, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu, India.
Polymeric biomaterials, particularly electrospun nanofibers, are increasingly central to the development of advanced wound dressings capable of supporting tissue regeneration while enabling real-time physiological monitoring. Chronic wounds associated with diabetes, vascular diseases, and cancer require continuous and personalized management, prompting the convergence of electrospun polymeric scaffolds with wearable biosensors and artificial intelligence (AI). These next-generation smart wound dressings utilize biocompatible polymer matrices functionalized with responsive sensing elements to monitor pH, temperature, moisture, oxygen saturation, and inflammatory biomarkers .
View Article and Find Full Text PDF