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Article Abstract
Heterotrophic nitrification aerobic denitrification represents an exceptionally efficient biological process for nitrogen removal, where nitrate reductase plays a pivotal role as the initial step. Using a crude enzyme solution, AtNR01 was able to degrade 80 % of 1 mg/mL NO-N within 20 min. Notably, AtNR01 can maintain robust performance over a wide range of temperatures and pH levels. In addition, when AtNR01 and its engineering bacteria are applied to the treatment of aquaculture wastewater, the degradation rate of NO-N can reach about 85 % within 10 h under the condition of low temperature 10 °C. Molecular docking results indicated that Nitrate binds to mvrA by the amino acid residues Ser 68 A and Phe 81 A and forms hydrogen bonds that help improve the stability of binding. These findings underscore the significant application potential and economic value of AtNR01 and its engineered counterpart in the treatment of low-temperature aquaculture wastewater.
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| http://dx.doi.org/10.1016/j.ijbiomac.2025.145967 | DOI Listing |
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