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Fixation plays a crucial role in green tea processing; however, the impact of different fixation degrees on the conversion of non-volatile and volatile metabolites remains unclear. This study explored the effects of mild, moderate, and severe fixation on green tea quality and identified 53 non-volatile metabolites, 60 volatile metabolites, and 17 objective flavor indicators. Moderate fixation (55 % leaf moisture) enhanced the umami and greenness of liquor by 38.91 % and 5.52 %, respectively. Using multivariate analyses, dose-over-threshold, and odor activity values, eight key non-volatile metabolites and three volatile metabolites were identified. Notably, moderate fixation reduced catechin and fatty acid oxidation, glutamic acid decarboxylation, and promoted flavonol glycoside degradation and glycoside hydrolysis. Moreover, it facilitated epigallocatechin gallate (9.49 %), theanine (3.05 %), and linalool formation and accumulation, thereby enhancing the taste and aroma of green tea. This study offers a foundation for optimizing green tea processing to improve tea quality.
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http://dx.doi.org/10.1016/j.foodchem.2025.145352 | DOI Listing |
Physiol Plant
September 2025
Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, Saskatchewan, Canada.
Dormancy release and germination of the seed are two separate, but continuous phases controlled by both external (e.g., light and temperature) and internal (e.
View Article and Find Full Text PDFFEBS Open Bio
September 2025
Department of Biochemistry, State University of Maringá, Maringá, Brazil.
Epigallocatechin-3-gallate (EGCG), the main catechin in green tea, is associated with antidiabetic and anti-obesity effects, although its acute hepatic actions remain unclear. We investigated short-term effects of EGCG (10-500 μm) using isolated perfused rat livers and complementary assays in mitochondrial, microsomal, and cytosolic fractions. EGCG markedly inhibited gluconeogenesis from lactate (up to 52%), glycerol (33%), and alanine (47%), while it stimulated glycolysis, glycogenolysis, and oleic acid oxidation (+42% total ketone bodies).
View Article and Find Full Text PDFPhytomedicine
August 2025
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; Yunnan Provincial Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China; Yunnan Provincial Engineering Research Center for Edible
Background: Walnut septum, a Juglans regia L. by-product with culinary-medicinal value, is a rich source of bioactive polyphenols. The chemical complexity and anti-colitis activities of these polyphenols remain uncharacterized.
View Article and Find Full Text PDFPestic Biochem Physiol
November 2025
Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address:
The cerambycid beetles are important components in the terrestrial ecosystem as they play a dual role in both degrading dying trees and killing healthy plants. The factors including human activity, habitat contraction, climate changes and pesticide use have been shaping the adaptation of beetles to host plants and the environment. As suggested in research on the functions of beetles' olfactory proteins, odorant binding proteins (OBPs) have been found to be involved in insecticide resistance other than chemoreception.
View Article and Find Full Text PDFJ Hazard Mater
September 2025
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address:
Cypermethrin, a common pyrethroid insecticide, raises significant ecological concerns due to its widespread use. In this study, a highly efficient cypermethrin-degrading strain, Rhodococcus sp. H-3, was isolated.
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