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High-energy X-rays can be employed to inspect fuel elements, even weld seams, cracks, and internal structural deformations of the components due to their density-sensitive characteristics and strong penetration capabilities. The quality of these X-rays is determined by various factors such as the X-ray conversion target, the cooling system for the conversion target, and the filter type, thereby influencing the detection accuracy. This study was conducted using an electron linear accelerator with an energy of 10 MeV and an average current of 150 μA. Firstly, we designed the conversion target material and geometry structure by Monte Carlo method. The results indicate that the optimal target material is tungsten and the optimum shape of the conversion target is a cylinder with dimensions of Φ 40 × 2.2 mm. Second, finite element analysis was applied to design the conversion target cooling system. After 60 min of electron beam irradiation, the local maximum temperature of the conversion target is approximately 1330 °C. Lastly, theoretical analysis and Monte Carlo simulations were used to design the X-ray filters. The result demonstrates that a double-layer filter consisting of 8 mm bismuth and 4 mm iron can increase the proportion of high-energy X-rays by 13.89 ± 0.05 %. Finally, the results of using the attenuation method to measure the X-ray energy spectrum produced by an electron linear accelerator indicate that without a filter 78.81 % of the X-rays are above 1 MeV and with a filter this proportion increases to 80.55 %.
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http://dx.doi.org/10.1016/j.apradiso.2025.111951 | DOI Listing |
Anal Chem
September 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361
Rolling circle amplification (RCA) has revolutionized nucleic acid detection owing to its isothermal simplicity. However, over two decades of clinical application have been hampered by off-target amplification and incompatibility with double-stranded DNA (dsDNA). Herein, a strategy, specifically cleavage of rationally designed DNA/RNA chimeric hairpin preprimer by dsDNA-targeted CRISPR/Cas12a to rlease ssRNA for initiating RCA (SCOPE-RCA), is proposed for nucleic acid identification of African swine fever virus (ASFV).
View Article and Find Full Text PDFArboviral infections, particularly Dengue and Zika, continue to rise at an alarming rate, with both viruses declared global health emergencies in 2024 and 2016, respectively. The NS5 RNA-dependent RNA polymerase (RdRp) of dengue virus (DENV) and Zika virus (ZIKV) is highly conserved, making nucleoside-based RdRp inhibitors a promising strategy for antiviral development. While nucleoside analogs have shown strong clinical potential, challenges such as cell permeability, the efficiency of triphosphate conversion, degradation, and mitochondrial toxicity remain.
View Article and Find Full Text PDFAnal Chem
September 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Deciphering the multicomponent of cell membranes at the single-cell level is critical for understanding pathological mechanisms such as tumor metastasis, yet remains technically daunting due to the membrane's nanoscale thickness and ultralow molecular abundance. Here, we introduce a surface-assisted vacuum ultraviolet laser desorption-ionization mass spectrometry imaging (SAVUVDI-MSI) platform that overcomes long-standing challenges of cytoplasmic interference and insufficient sensitivity. Leveraging the nanoscale depth profiling capability of VUV-LDI, we achieve precise ablation of a single-cell membrane.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Ophthalmology, Tianjin Medical University General Hospital, International Joint Laboratory of Ocular Diseases (Ministry of Education), State Key Laboratory of Experimental Hematology, Tianjin Key Laboratory of Ocular Trauma, Laboratory of Molecular Ophthalmology, Tianjin Medical Univer
Ocular fibrosis, a severe consequence of excessive retinal wound healing, can lead to vision loss following retinal injury. Proliferative vitreoretinopathy (PVR), a common form of ocular fibrosis, is a major cause of blindness, characterized by the formation of extensive fibrous proliferative membranes. Understanding the cellular origins of PVR-associated fibroblasts (PAFs) is essential to decipher the mechanisms of ocular wound healing.
View Article and Find Full Text PDFAnal Chem
September 2025
Institute for Advanced Interdisciplinary Research (iAIR), School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China.
Compared with efficient anodic luminol electrochemiluminescence (ECL), the disadvantage of cathodic ECL is that luminol cannot be electrochemically oxidized in a direct manner, and the conversion efficiency of dissolved oxygen (DO) as the coreactant to reactive oxygen species (ROS) is poor, which limits its application. Therefore, it is necessary to develop a functional catalyst suitable for the luminol-DO ECL system to directly trigger cathodic ECL. In this study, a coordination microenvironment modulation strategy was proposed.
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