Influence of Different Levels of Ginger-Derived Liquid Protease on Growth Performance, Nutrient Digestibility, Immune Organs and Gut pH in Broiler Chickens.

This study assessed the effects of ginger-derived liquid protease (GDPE) supplementation on growth performance, nutrient digestibility, gut health, and histology in broiler chickens (n = 360) over 35 days in a completely randomized design. Four dietary treatments were provided: a control diet and three test groups with varying GDPE concentrations (0.25 ml/kg, 0.5 ml/kg, and 1 ml/kg). Results showed that GDPE supplementation, especially at 0.5 ml/kg (GDPE2) and 1 ml/kg (GDPE3), significantly (p < 0.05) improved feed conversion ratio, dressing percentage, and immune organ weights. The highest (p < 0.05) weight gain occurred in GDPE2, while the lowest (p < 0.05) feed intake was observed in GDPE3. Supplementing with GDPE also reduced (p < 0.05) gut pH and enhanced (p < 0.05) nutrient digestibility, particularly ether extract. Apparent metabolizable energy (AME) increased with GDPE supplementation, reaching its peak in GDPE3 (p < 0.05). Overall, 1 ml/kg GDPE improved growth, nutrient digestibility, immune function, and gut health in broilers.