Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Magnetic resonance spectroscopy (MRS) enables the non-invasive quantification of brain metabolites, and its reliability is crucial for accurate interpretation of disease state. This study assessed the test-retest precision of phosphorus-31 (P)-MRS and hydrogen (H)-MEscher-GArwood Point RESolved Spectroscopy (MEGA-PRESS) in measuring P metabolites, γ-aminobutyric acid (GABA), and glutathione (GSH) using a 3T multi-nucleus magnetic resonance imaging (MRI) system.
Methods: In total, 32 participants, who underwent two scanning sessions within three days, using two dimensional (2D)-chemical shift imaging (CSI)-P-MRS and H-MEGA-PRESS sequences, were enrolled in the study. γ-aminobutyric acid and macromolecules (GABA+), glutamate and glutamine (Glx), GSH, and 12 P metabolites were analyzed using the MATLAB-based tool Gannet and jMRUI software. Precision was assessed based on the coefficients of variation (CVs) and Bland-Altman plots.
Results: The results revealed that potential of hydrogen (pH) and phosphocreatine (PCr) showed the greatest stability as evidenced by low CVs, suggesting reliable measurements across sessions. The adenosine triphosphates (ATPs) showed considerable stability. Conversely, metabolites, such as phosphomonoesters (PMEs) and phosphodiesters (PDEs), located to the left of PCr, showed reduced stability, while glycerophosphatidylcholine (GPTC) had the highest CV, indicating significant variability in clinical practice. Among the various brain regions, intermediate areas such as the temporal lobe and thalamus exhibited greater stability than peripheral regions such as the frontal and occipital lobes. Single-voxel MEGA-PRESS measurements showed that Glx and GABA+ had higher precision than GSH.
Conclusions: Both the P-MRS and H-MEGA-PRESS sequences showed high precision in measuring brain metabolites, but some metabolites showed higher stability than others. These results are crucial for exploring the clinical and research applications of these methods, and provide a solid foundation for subsequent investigations.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994524 | PMC |
| http://dx.doi.org/10.21037/qims-24-1853 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Brain Metabolite Concentrations in Neonates with Congenital Heart Disease Before and After Cardiac Surgery.
Neuroimage
September 2025
Developing Brain Institute, Children's National Hospital, Washington, D.C. USA; Department of Radiology, The George Washington University School of Medicine and Health Sciences, Washington, D.C. USA; Department of Pediatrics, The George Washington University School of Medicine and Health Sciences, W
The purpose of this study was to compare brain metabolite concentrations between healthy and CHD neonates before and after cardiac surgery. Healthy term newborns and neonates with CHD were recruited prospectively. T-weighted brain images and MRS data were acquired in the cerebellum, right frontal lobe and basal ganglia.
View Article and Find Full Text PDFGlymphatic-meningeal lymphatic system, a new therapeutic target for sleep disorders.
Neuroscience
September 2025
Nanjing Research Institute of Electronic Technology, Nanjing 210039, China. Electronic address:
Sleep disorders encompass a range of diseases and symptoms that disrupt individual sleep patterns, degrade sleep quality, and diminish sleep efficiency. Currently, the mechanisms governing sleep regulation and the etiology of sleep disorders remain unclear, leading to clinical treatments that are primarily symptomatic due to the absence of precise intervention methods. Recent studies suggest that glymphatic-meningeal lymphatic route is responsible for the clearance of macromolecular metabolites from the brain, thus playing a pivotal role in maintaining sleep homeostasis and circadian rhythm.
View Article and Find Full Text PDFBiopsychosocial and Environmental Factors that Impact Brain-Gut-Microbiome Interactions in Obesity.
Clin Gastroenterol Hepatol
August 2025
Vatche and Tamar Manoukian Division of Digestive Diseases, University of California, Los Angeles; Goodman Luskin Microbiome Center, University of California, Los Angeles; G. Oppenheimer Center for Neurobiology of Stress and Resilience, University of California, Los Angeles; David Geffen School of Me
Background: Despite significant advances in the understanding of the pathogenesis of obesity and influencing factors, its prevalence continues to increase at an alarming rate. Social determinants of health (SDOH) encompass a broad range of psychosocial and environmental factors, including economic stability, education, access to healthcare, social support, isolation, neighborhood disadvantage, discrimination, early life adversity, and stress, all of which have been recognized to significantly increase the risk of obesity.
Aim: This review aims to elucidate the intricate relationship between SDOH and biological mechanisms related to the brain-gut-microbiome (BGM) system that lead to altered eating behaviors and obesity.
Gut microbial production of imidazole propionate drives Parkinson's pathologies.
Nat Commun
September 2025
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Parkinson's disease (PD) is characterized by the selective degeneration of midbrain dopaminergic neurons and aggregation of α-synuclein. Emerging evidence implicates the gut microbiome in PD, with microbial metabolites proposed as potential pathological mediators. However, the specific microbes and metabolites involved, and whether gut-derived metabolites can reach the brain to directly induce neurodegeneration, remain unclear.
View Article and Find Full Text PDFLimosilactobacillus reuteri-butyrate axis in depression therapy: A key pathway discovered through a novel preclinical human flora-associated animal model.
Pharmacol Res
September 2025
School of Pharmacy, Second Military Medical University, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, Shanghai 200433, China. Electronic address:
The transition from preclinical to clinical drug development is critically impeded by interspecies disparities, which limit the predictive validity of preclinical efficacy for human outcomes. To address this limitation, we established a human flora-associated depression rat (HFADR) model through fecal microbiota transplantation (FMT). The HFADR model bridges the preclinical-clinical translation by recapitulating conserved microbial-host interactions identified through multi-omics analysis in a chronic unpredictable mild stress (CUMS) rat model and in patients with major depressive disorder.
View Article and Find Full Text PDF