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Article Abstract
To investigate the cadmium (Cd) uptake mechanism in the roots of Coptis chinensis Franch., we utilized non-invasive micro-test technology (NMT) to analyze Cd and H fluxes, alongside ICP-MS to assess Cd content and fluxes under various inhibitors and ion channel blockers. Results revealed that Cd uptake primarily occurs in the root meristematic zone. Cd addition significantly inhibited H influx. The P-type ATPase inhibitor NaVO and the metabolism inhibitor CCCP both reduced Cd fluxes and Cd content in roots of C. chinensis Ca channel blocker LaCl exhibited more pronounced inhibition on Cd flux than K channel blocker TEA, indicating Cd uptake mainly via Ca channels. Therefore, active transport and Ca channels may play an important role in Cd uptake in C. chinensis roots.
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