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Article Abstract
Purpose: Embryo engineering requires a large number of oocytes, which undergo in vitro maturation (IVM). Understanding how to select the best quality oocytes is key to improving IVM efficiency. Oocytes have different germinal vesicle (GV) chromatin configurations, which may explain the heterogeneity in oocyte quality during IVM. However, no reports have categorized, the chromatin configuration of rat GVs or evaluated, the association between the chromatin configuration and oocytes development.
Methods: The GV chromatin configuration of rat oocytes was divided into seven types according to the degree of chromatin compaction: non-surrounded nucleolus (NSN), prematurely condensed NSN, partly NSN, partly surrounded nucleolus (SN-1), SN-1, condensed SN-1, and aggregated (SN-2). The chromatin configuration distribution was compared during the different stages of oocyte growth and maturation. We also analyzed the changes in the chromatin configuration at different GV stages during IVM. Moreover, the factors affecting the chromatin configuration were analyzed.
Results: The SN-2 configuration increased with rat oocyte growth and maturation, suggesting that SN-2 facilitates oocyte development. RNA transcription activity in rat oocyte GVs was inversely correlated with oocyte IVM.
Conclusions: The SN-2 chromatin configuration was related to rat oocyte growth and maturation. RNA transcription activity in rat oocytes in the GV stage was inversely correlated with oocyte maturation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825056 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0312241 | PLOS |
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