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http://dx.doi.org/10.1016/j.lanmic.2024.101014 | DOI Listing |
J Infect Dev Ctries
August 2025
Department of Medical Microbiology, Faculty of Medicine, Ege University, Izmir 35100, Turkey.
Introduction: The aim of this study was to compare the performance of different clinical specimens-nasopharyngeal (NP) swabs collected by healthcare professionals (HCP-NP), self-collected nasal swabs (Sc-N), and saliva samples (S)-in diagnostic tests for investigating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and influenza A/B RNA.
Methodology: These clinical samples were collected from 404 symptomatic cases and tested with the SARS-CoV-2 and influenza A/B RNA tests on the cobas 6800 System of Roche Molecular Systems (Roche Molecular Systems, Pleasanton, USA). The SARS-CoV-2 or influenza virus infection status was determined for all patients based on the predefined criteria and corresponding algorithms.
J Microbiol Methods
September 2025
Dynamics of Respiratory Infections Group, Helmholtz Centre for Infection Research-HZI Braunschweig, Braunschweig, Germany; Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, German Center for Lung Research (DZL), BREATH, Hannover, Germany.
Purpose: The accuracy of oral microbiome research depends significantly on specimen sampling protocols, as well as their storage and preservation. Traditional methods, such as freezing, may not only involve logistical hurdles but can also impact the quality of microbial data, leading to difficulties in the comparability between different studies. This study evaluates the effectiveness of the room temperature nucleic acid preservation protocol using DNA/RNA Shield buffer as compared to standard freezing in preserving oral microbial communities over the course of 7 days.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
September 2025
College of Chemistry and Molecular Sciences, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430072, P.R. China.
Peptide-based biosensors are widely used for in vitro detection of protease activity but often suffer from the limited sensitivity, poor accuracy, and incompatibility with point-of-care testing (POCT) devices. Herein, we developed a versatile deoxyribozyme (DNAzyme)-amplified protease-sensing (DP) platform that integrates the positively charged oligopeptides with a negatively charged DNAzyme biocatalyst for highly-sensitive protease detection. The system leverages the electrostatic peptide-DNAzyme interactions to inhibit DNAzyme catalytic activity, which is reactivated upon the protease-triggered peptide hydrolysis, thus enabling an efficient signal amplification via the successive cleavage of DNAzyme substrate.
View Article and Find Full Text PDFFront Public Health
September 2025
Department of Family and Community Medicine, Penn State University College of Medicine, Hershey, PA, United States.
Background: The World Health Organization recommends at-home management of mild COVID-19. While our preliminary evaluation provided evidence for saline nasal irrigation (SNI) and gargling in COVID-19, an update and risk-benefit assessment for self-care in Omicron infection is warranted, from treatment and preparedness perspectives, as new SARS-CoV-2 variants continuously emerge, while symptoms overlap with those of common colds and other upper respiratory tract infections.
Methods: Systematic literature searches for preclinical and clinical studies involving Omicron infection and saline, bias assessment, and review of outcomes (benefits, risks).
ACS Omega
August 2025
VinUni-Illinois Smart Health Center, VinUniversity, Hanoi 100000, Vietnam.
Accurate and accessible glucose detection is essential for clinical diagnostics, point-of-care testing, food safety, and biosensing applications. In this study, we present a simple, scalable, and dual-mode glucose sensor that integrates commercial potassium permanganate (KMnO) with glucose oxidase to enable sensitive and selective detection in the clinically critical range of 1-5 mM. Leveraging the strong oxidative power and distinct optical characteristics of KMnO, the sensor operates via both absorbance measurement at 400 nm and visual colorimetric analysis, displaying a clear color change from purple to pink and yellow upon reaction with glucose.
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