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The study aimed to evaluate the synergistic interaction of organic acids (OAAs) or lemon extract (LE) plus mild heat (MH; 55 °C) against O157:H7, serovar Typhimurium, and inoculated in beetroot and watermelon juices. A mixed culture cocktail of . O157:H7, . Typhimurium or . was inoculated in beetroot or watermelon juice, followed by treatments with MH, citric acid MH, malic acid MH, tartaric acid MH, and LE MH. Approximately 2.0-log reductions in the number of . O157:H7, . Typhimurium, and . were observed when these bacteria were heated in juices at 55 °C for 5 min. A combination of 1.0% OAAs or 20% LE and MH (55 °C) for 5 min resulted in an additional log-reduction in the count of . O157:H7, . Typhimurium, and . by 2.2-5.0, 4.5-5.0, and 1.5-5.0, respectively.
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http://dx.doi.org/10.1007/s10068-024-01539-7 | DOI Listing |
EBioMedicine
September 2025
Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford, United Kingdom; NIHR Oxford Biomedical Research Centre, Oxford, United Kingdom; Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom.
Background: Invasive non-typhoid Salmonella (iNTS) is a leading cause of morbidity and mortality in sub-Saharan Africa. We assess the safety and immunogenicity of an outer membrane vesicle vaccine (iNTS-GMMA) derived from the two most common serovars, S. Enteritidis (SEn) and S.
View Article and Find Full Text PDFJ AOAC Int
September 2025
Office of Laboratory Operations and Applied Science, Human Foods Program, U.S. Food and Drug Administration, College Park, Maryland.
Background: As a leading cause of foodborne illness worldwide, detection of Salmonella enterica subsp. enterica serovar Typhimurium is essential for food safety and public health.
Objective: This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella ser.
Front Immunol
September 2025
Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
is a leading cause of foodborne illness in the United States and worldwide. This enteric pathogen deploys various mechanisms to evade the intestinal mucosal barrier to enhance its survival and further infect systemic tissues. Commercially available vaccines against are currently restricted to the serovar Typhi, while none are currently approved for non-typhoidal (NTS) serovars, which are becoming increasingly resistant to antibiotics.
View Article and Find Full Text PDFFood Microbiol
January 2026
Department of Food Science, Purdue University, 745 Agriculture Mall Dr., West Lafayette, IN, United States.
Recent foodborne illness outbreaks linked to lettuce and cucumbers have prompted research into the sources of contamination and the risks of pathogen internalization within the edible portions. This study assessed the colonization and presence of Escherichia coli O157:H7 and Salmonella enterica ser. Typhimurium in romaine lettuce (True Heart, Rio Bravo) and cucumber (Mini-Me, Gherkin) varieties under greenhouse conditions.
View Article and Find Full Text PDFMicroorganisms
August 2025
School of Earth, Environmental, and Marine Sciences, University of Texas Rio Grande Valley, 1201 W University Dr, Edinburg, TX 78539, USA.
Hydroponic nutrient solution (HNS) has been established as an ideal conduit for pathogen contamination and proliferation. This study evaluated the efficacy of lactic acid bacteria and their metabolites in mitigating the risk of foodborne pathogens in HNS when compared to conventional chemical treatments. Hoagland's HNS were prepared according to the manufacturer's instructions and inoculated with Typhimurium, 0157:H7, and at 10 CFU/mL cell concentration.
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