Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Sporotrichosis is a chronic granulomatous infection of the skin and subcutaneous tissue that can affect any organ through lymphatic spread. The prevalence of sporotrichosis infections is increasing and its treatment is challenging as there are no unified and standard diagnostic techniques or antifungal medications. Controlling further spread requires a rapid diagnosis. Assessment of clinical symptoms, histological analysis, serological testing, and pathogen culture are all necessary for the diagnosis of sporotrichosis. However, these procedures are unable to identify the species. The development of safe, reliable, and species-specific diagnostic techniques is essential.
Objective: To establish and evaluate a new quantitative real-time PCR assay for the rapid diagnosis of sporotrichosis and to identify relevant species.
Methods: Polymorphisms in calmodulin (CAL) gene sequences and the internal transcribed spacer (ITS) were used in a quantitative real-time PCR assay to identify S. globosa, S. schenckii, and non-target species.
Results: The quantitative real-time PCR assay had 100% sensitivity and specificity. The limit of detection was 6 fg/µl. Thirty-four clinical specimens were verified to be infected with S. globosa with a 100% positive detection rate.
Conclusions: The quantitative PCR technique developed in this study is a quick, accurate, and targeted method of identifying S. globosa based on polymorphisms in CAL sequences and ITS. It can be used for a prompt clinical diagnosis to identify S. globosa in clinical specimens from patients with sporotrichosis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11323652 | PMC |
| http://dx.doi.org/10.1186/s12879-024-09714-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the olfactory repertoire of the ecologically important parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae): from gene structures to expression analysis.
J Econ Entomol
September 2025
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
The ability of parasitoid wasps to precisely locate hosts in complex environments is a key factor in suppressing pest populations. Chemical communication plays an essential role in mediating insect behaviors such as locating food sources, hosts, and mates. Odorant receptors (ORs) are the key connection between external odors and olfactory nerves.
View Article and Find Full Text PDFPeroxisome proliferator-activated receptor pathway and key genes in cerebellar dysfunction induced by chronic intermittent alcohol exposure: a transcriptomic study.
Neurol Res
September 2025
Department of Neurology, The First Affiliated Hospital of Jiamusi University, Jiamusi, People's Republic of China.
Background: We conducted a transcriptomic analysis to examine cerebellar transcriptional changes in a mouse model of chronic intermittent alcohol exposure.
Methods: We established a mouse model of chronic intermittent alcohol exposure and conducted a cerebellar transcriptomic analysis. After identifying differentially expressed genes, we analyzed pathway enrichment using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology.
Unlabelled: Passive Acoustic Mapping (PAM) is rapidly emerging as a ubiquitous tool for real-time localization and monitoring of therapeutic ultrasound treatments involving cavitation in the context of safety or efficacy. The ability of PAM to spatially quantify and resolve cavitation activity offers a unique opportunity to correlate the energy of cavitation phenomena with locally observed bioeffects.
Objective: We aim to develop methods of measuring and reporting spatio-temporally varying cavitation energies that are energy-preserving, device-independent, and adequately normalized to the volume of tissue being affected by the reported cavitation activity.
Effects and Mechanisms of Lactiplantibacillus plantarum G83 on Enterotoxigenic Escherichia coli (ETEC)-Induced Intestinal Inflammation.
Probiotics Antimicrob Proteins
September 2025
Key Laboratory of the Ministry of Education for Wildlife and Plant Resources Conservation in Southwest China, College of Life Sciences, China West Normal University, Nanchong, Sichuan, China.
Enterotoxigenic Escherichia coli (ETEC) is a prevalent intestinal pathogen that significantly impacts both human and animal health. G83, isolated from giant panda feces, has demonstrated notable probiotic properties. In this study, C57BL/6 J mice were randomly divided into Control, ETEC, and G83 groups.
View Article and Find Full Text PDFLabel-Free Dynamic Single-Molecule Sensing of Alpha-2-Macroglobulin in Complex Matrices.
ACS Sens
September 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
Alpha-2-macroglobulin (A2M) is a critical biomarker implicated in inflammation, immune regulation, coagulation, and various pathological conditions such as liver fibrosis, neurodegenerative diseases, and cancers. However, its precise quantification remains challenging due to complex conformational dynamics, subtle abundance fluctuations, and interference from plasma proteins. Here, we present a label-free dynamic single-molecule sensing (LFDSMS) strategy for the sensitive and specific detection of A2M.
View Article and Find Full Text PDF