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The adsorbents used to remove taint compounds from wine can also remove constituents that impart desirable color, aroma, and flavor attributes, whereas molecularly imprinted polymers (MIPs) are tailor-made to selectively bind one or more target compounds. This study evaluated the potential for MIPs to ameliorate smoke taint in wine via removal of volatile phenols during or after fermentation. The addition of MIPs to smoke-tainted Pinot Noir wine (for 24 h with stirring) achieved 35-57% removal of guaiacol, 4-methylguaiacol, cresols, and phenol, but <10% of volatile phenol glycoconjugates were removed and some wine color loss occurred. Of the MIP treatments that were subsequently applied to Semillon and Merlot fermentations or wine, MIP addition post-inoculation of yeast yielded the best outcomes, both in terms of volatile phenol removal and wine sensory profiles. Despite some impact on other aroma volatiles and red wine color, the findings demonstrate that MIPs can ameliorate smoke-tainted wine.
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http://dx.doi.org/10.1021/acs.jafc.4c03912 | DOI Listing |
J Agric Food Chem
September 2025
School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, Adelaide, South Australia 5064, Australia.
Smoke taint is a fault that can occur in wines made from smoke-affected grapes. Smoke taint research has largely focused on chemical and sensory analysis and mitigation strategies, with limited attention to underlying molecular responses. We performed RNA sequencing on berries from cultivars Shiraz and Chardonnay after controlled smoke exposure.
View Article and Find Full Text PDFJ Agric Food Chem
August 2025
Technical University of Munich, TUM School of Natural Sciences, Department of Chemistry, Technical University of Munich, Lichtenbergstraße 4, Garching 85748, Germany.
Among the off-flavors occasionally found in fermented cocoa, a smoky taint is common. While major contributors to the off-flavor are already known, their source has not been fully clarified: wood smoke contact during drying and overfermentation are currently discussed. Odorant screening by gas chromatography-olfactometry and aroma extract dilution analysis applied to a cocoa sample smoked in a worst-case scenario confirmed 2-methoxyphenol, 3- and 4-methylphenol, 3- and 4-ethylphenol, and 3-propylphenol as important smoky odorants and additionally suggested 2,6-dimethoxyphenol as a potential off-flavor compound.
View Article and Find Full Text PDFMolecules
June 2025
Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA.
This study investigated the correlation between five primary volatile phenols (VPs) and their glycosides in smoke-exposed and non-smoke-exposed Pinot noir wines to assess and identify potential markers for smoke taint. The results showed that all putative VP-glycosides in smoke-exposed wines were higher than in non-smoke-exposed wines, with a fold change ranging from 2.11 to 31.
View Article and Find Full Text PDFJ Exp Bot
July 2025
Department of Viticulture and Enology, University of California Davis, Davis, CA, 95616, USA.
Wildfires are an increasingly urgent threat to viticulture, with smoke exposure leading to smoke taint, an off-flavor in wine characterized by undesirable "smoky" and "ashy" notes that can render grapes unmarketable and cause major economic losses. Smoke-derived volatile phenols (VPs) in grape berries, metabolized into stable, non-volatile glycoconjugates via glycosyltransferases (GTs), underlie smoke taint formation. Here, we present the dwarf grapevine cultivar Pixie as a model system for smoke taint research.
View Article and Find Full Text PDFJ Agric Food Chem
July 2025
The Australian Wine Research Institute, P.O. Box 46, Glenside, South Australia 5065, Australia.
Thiophenols were recently identified in wine as undesirable compounds contributing to the ashy aftertaste associated with smoke taint. These potential odorants enhanced the unpleasant sensory properties associated with smoke taint at ultratrace (ng/L) levels when present in wine with volatile phenols, but accurate quantification of volatile sulfur compounds at this concentration remains challenging. A simple, stable isotope dilution assay (SIDA) method employing ultrahigh-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was developed and validated for accurate quantification of six thiophenols (benzenethiol, 2-methylbenzenethiol, 3-methylbenzenethiol, 4-methylbenzenethiol, 2-methoxybenzenethiol, and methylsulfanylbenzene) using N-ethylmaleimide (NEM) as a derivatizing agent and a chemically matched deuterated analogue (-benzenethiol) as the internal standard.
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