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Article Abstract
Background: The accurate and rapid detection of blood lead concentration is of paramount importance for assessing human lead exposure levels. Fluorescent protein-based probes, known for their high detection capabilities and low toxicity, are extensively used in analytical sciences. However, there is currently a shortage of such probes designed for ultrasensitive detection of Pb, and no reported probes exist for the quantitative detection of Pb in blood samples. This study aims to fill this critical void by developing and evaluating a novel fluorescent protein-based probe that promises accurate and rapid lead quantification in blood.
Results: A simple and small-molecule fluorescent protein-based probe was successfully constructed herein using a peptide PbrBD designed for Pb recognition coupled to a single fluorescent protein, sfGFP. The probe retains a three-coordinate configuration to identify Pb and has a high affinity for it with a K' of 1.48 ± 0.05 × 10 M. It effectively transfers the conformational changes of the peptide to the chromophore upon Pb binding, leading to fast fluorescence quenching and a sensitive response to Pb. The probe offers a broad dynamic response range of approximately 37-fold and a linear detection range from 0.25 nM to 3500 nM. More importantly, the probe can resist interference of metal ions in living organisms, enabling quantitative analysis of Pb in the picomolar to millimolar range in serum samples with a recovery percentage of 96.64%-108.74 %.
Significance: This innovative probe, the first to employ a single fluorescent protein-based probe for ultrasensitive and precise analysis of Pb in animal and human serum, heralds a significant advancement in environmental monitoring and public health surveillance. Furthermore, as a genetically encoded fluorescent probe, this probe also holds potential for the in vivo localization and concentration monitoring of Pb.
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| http://dx.doi.org/10.1016/j.aca.2024.342580 | DOI Listing |
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