Searching for the Virulence-contributing Genes of the by Transcriptome Analysis.

Pathogens

State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Chi

Published: January 2024


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

is a fungal pathogen that causes rice blast. Plant metabolites such as plant hormones and phytoalexin can promote or inhibit the rice blast infection. To study the effect of plant metabolites on , we selected salicylic acid (SA), abscisic acid (ABA), and a phytoalexin sakuranetin to treat grown on the medium. Through the analysis of transcriptome data, 185 and 38 genes, 803 and 156 genes, and 1525 and 428 genes were up- or down-regulated after SA, ABA, or sakuranetin treatment. Among these differentially expressed genes (DEGs), most of them were annotated to the cellular process and metabolic process in the biological process category and binding and catalytic activity in the molecular function category by GO analysis. According to KEGG pathway analysis, metabolism is the pathway with the highest number of DEGs, and the main enriched pathway is carbohydrate, lipid, and amino acid metabolism. In addition, we also found two ABA-induced up-regulated genes that may contribute to infection from the transcriptome data. We verified their expressions in that infected rice.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10891974PMC
http://dx.doi.org/10.3390/pathogens13020105DOI Listing

Publication Analysis

Top Keywords

rice blast
8
plant metabolites
8
transcriptome data
8
genes
6
searching virulence-contributing
4
virulence-contributing genes
4
genes transcriptome
4
analysis
4
transcriptome analysis
4
analysis fungal
4

Similar Publications

Discovery and phylogeny of a ricin-B-like domain from rice.

Carbohydr Res

September 2025

Laboratory for Biochemistry & Glycobiology, Ghent University, Department of Biotechnology, Ghent, Belgium. Electronic address:

Lectins are carbohydrate-binding proteins which play key roles in various biological processes, including cell signaling, pathogen recognition and development. Previous research conducted on ricin-B lectin domains and carbohydrate-binding modules of family 13 (CBM13) illustrated the striking resemblances between these two groups of protein domains. In this study, we report on the discovery, identification and putative biochemical characteristics of a ricin-B-like domain that is unique for GH27 enzymes from land plants, identified in the OsAPSE enzyme from Japanese rice (Oryza sativa L.

View Article and Find Full Text PDF

Introduction: Rice is an important food crop but is susceptible to diseases. However, currently available spot segmentation models have high computational overhead and are difficult to deploy in field environments.

Methods: To address these limitations, a lightweight rice leaf spot segmentation model (MV3L-MSDE-PGFF-CA-DeepLabv3+, MMPC-DeepLabv3+) was developed for three common rice leaf diseases: rice blast, brown spot and bacterial leaf blight.

View Article and Find Full Text PDF

Just as Gregor Mendel's laws of inheritance laid the foundation for modern genetics, the emergence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas systems has catalyzed a new era in precision genome engineering. CRISPR/Cas has revolutionized rice ( L.) breeding by enabling precise, transgene-free edits to improve yield, nutrition, and stress tolerance.

View Article and Find Full Text PDF

OsPIL1 Differentially Modulates Rice Blast Resistance Through Integrating Light or Darkness During Magnaporthe oryzae Infection.

Plant Cell Environ

September 2025

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming, China.

Light and darkness are critical environmental factors that regulate plant immune responses. OsPIL1, a phytochrome-interacting factor-like protein, has been implicated in rice immunity against Magnaporthe oryzae, although its underlying mechanism remains unclear. This study aimed to dissect how OsPIL1 integrates light or darkness to modulate rice immunity.

View Article and Find Full Text PDF