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The rodent running-wheel recording apparatus is a reliable approach for studying circadian rhythm. This study demonstrated how to construct a simple and intelligent running-wheel recording system. The running wheel was attached to the cage's base, whereas the Hall sensor was attached to the cage's cover. Then, the RJ25 adaptor relayed the running signal to the main control board. Finally, the main control board was connected to the USB port of the computer with the USB connection. Data were collected using the online-accessible, self-created software Magturning. Through Magturning, generated data were saved and exported in real time. Afterward, the device was validated by collecting data on the locomotor activities of mice under different light conditions. In conclusion, this new device can record circadian activity of rodents. Our device is appropriate for interdisciplinary investigations related to biological clock research.
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http://dx.doi.org/10.1002/ame2.12381 | DOI Listing |
PLoS One
September 2025
Department of Biological Sciences, University of Limerick, Limerick, Ireland.
This study investigates the interaction between circadian rhythms and lipid metabolism disruptions in the context of obesity. Obesity is known to interfere with daily rhythmicity, a crucial process for maintaining brain homeostasis. To better understand this relationship, we analyzed transcriptional data from mice fed with normal or high-fat diet, focusing on the mechanisms linking genes involved with those regulating circadian rhythms.
View Article and Find Full Text PDFAging Cell
September 2025
Department of Epidemiology, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University, New Orleans, Louisiana, USA.
Epigenetic clocks have emerged as promising biomarkers of aging, but their responsiveness to lifestyle interventions and relevance for short-term changes in cardiometabolic health remain uncertain. In this study, we examined the associations between three epigenetic aging measures (DunedinPACE, PCPhenoAge acceleration, and PCGrimAge acceleration) and a broad panel of cardiometabolic biomarkers in 144 obese participants from the MACRO trial, a 12-month weight-loss dietary intervention comparing low-carbohydrate and low-fat diets. At pre-intervention baseline, DunedinPACE was significantly associated with several cardiometabolic biomarkers (FDR [false discovery rate] < 0.
View Article and Find Full Text PDFJ Chem Theory Comput
September 2025
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
The proton (or hydrogen atom) transfer via tunneling plays a key role in chemical and biological processes. However, our understanding of multiple motion or proton concerted tunneling is very limited. Herein, we find that the weak dispersion interaction in the formic acid dimer (FAD)-fluorobenzene (PhF) system does not change the double proton transfer (DPT) barrier in FAD, but induces the FAD swing coupled with DPT.
View Article and Find Full Text PDFNPJ Biol Timing Sleep
September 2025
Healthy Living Spaces Lab, Institute for Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Aachen, Germany.
The retinal photopigment melanopsin is also expressed in subcutaneous white adipose tissue (scWAT). Through melanopsin, light can modulate scWAT metabolism, but its impact on circadian phase is unclear. In vitro exposure of murine scWAT to bright light at different times over 24 h did not elicit phase shifts, unlike the response to corticosterone.
View Article and Find Full Text PDFFASEB J
September 2025
Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, East China Normal University, Shanghai, China.
The molecular clock exhibits distinct characteristics across various tissues and can be synchronized by particular stimuli. Furthermore, there is an intricate interplay among the molecular clocks within different tissues. In this context, we present an overview of the tissue-specific molecular clock and discuss pivotal nonphotic regulators that govern the host's circadian rhythms and metabolic processes.
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