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Iron nanoparticles (FeNPs) are commonly used in various industrial processes, leading to their release into the environment and eventual entrance into wastewater treatment plants (WWTPs). FeNPs undergo dissolution, migration, and transformation in WWTPs, which can potentially affect the stable operation of anaerobic ammonia oxidation (anammox) systems and may be discharged with wastewater or biomass. To better understand the fate of FeNPs in anammox systems, exposure experiments were conducted using anammox granular sludges (AnGS) and FeNPs. Results demonstrated that FeNPs released Fe upon contact with water, with a portion being bound to functional groups in extracellular polymeric substances (EPS) and the rest entering the bacteria to form highly absorbable substances. A significant amount of FeNPs was observed to cover the surface of AnGS or aggregate and deposit at the bottom of the reactor, eventually converting into FeO and stably existing within the anammox system. The findings of this study clarify the fate of FeNPs in anammox systems and provide important insights into the stable operation of anammox systems under FeNPs exposure.
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http://dx.doi.org/10.1016/j.jenvman.2023.119323 | DOI Listing |
J Hazard Mater
September 2025
School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou 221116, PR China. Electronic address:
Microplastics (MPs) and the plastisphere they form pose substantial ecological risks in aquatic environments and wastewater treatment processes. As a unique niche, the evolution of plastisphere in anaerobic ammonium oxidation (anammox) systems remains poorly understood. This study investigated the physicochemical evolution of polyethylene terephthalate (PET) MPs and microbial succession within the plastisphere during a 30-day incubation with anammox granular sludge.
View Article and Find Full Text PDFEnviron Res
September 2025
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, PR China.
Partial denitrification coupled with anammox (PD/A) has emerged as a promising low-carbon strategy for energy-efficient nitrogen removal from municipal wastewater. However, the reactivation of PD/A systems following operational disturbances remains challenging, particularly under continuous-flow conditions, where microbial interactions and process stability are more complex than in sequencing batch reactors. This study systematically and first evaluated the recovery dynamics of a continuous-flow PD/A process seeded with low-activity granular sludge stored at 4 °C for three months.
View Article and Find Full Text PDFWater Res
August 2025
College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Ocean Academy, Zhejiang University, Zhoushan, 316021, China. Electronic address:
Salinity-driven nitrogen removal mechanisms in iron-carbon CWs (Fe-C-CWs) remain poorly understood for aquaculture tailwater management. Through a 155-day trial under four salinities (designated as S0, S10, S20, and S30), result showed that S20 achieved optimal removals of total nitrogen (84.9 ± 3.
View Article and Find Full Text PDFEnviron Res
August 2025
National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, PR
The stress of high-concentration Fe(III) severely restricts the anaerobic ammonium oxidation (anammox) engineering application. Herein, we systematically investigated the mitigating mechanisms of biochar-mediated anammox system against Fe(III) stress by constructing a batch reaction system with a gradient Fe(III) concentration (0-100 mg/L). The results showed that biochar notably mitigated Fe(III) toxicity by synergizing multiple pathways, such as physical adsorption, chemical reduction, and biological sheltering.
View Article and Find Full Text PDFJ Hazard Mater
August 2025
School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, China; Hua An Biotech Co., Ltd., Foshan 528300, China. Electronic address:
Rare earth mining activities cause severe nitrogen pollution in watersheds, yet the residual hazardous rare earth elements (REEs) toxicity in tailings wastewater challenges biological nitrogen removal technology. This work demonstrated that introducing low-dose Fe(II) into partial denitrification/anammox (PD/A) system significantly alleviated REEs-induced stress on anammox consortia via detoxification and physical barrier reinforcement. The PD/A bioreactor with 15 mg/L Fe(II) (R1) was compared against a control without Fe(II) for real rare earth tailings wastewater treatment.
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