Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Nephrotoxicity induced by aristolochic acid I (AAI) is related to redox stress and apoptosis. Apurinic/apyrimidine endonuclease 1 (APE1) has antioxidant and anti-apoptotic effects. This study investigated the potential role of APE1 in AAI-induced nephrotoxicity. Renal injury was successfully induced in C57BL/6J mice by intraperitoneal injection of AAI every other day for 28 days. Expressions of APE1, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase 1 (HO-1) in renal tissues of the model mice was inhibited, accompanied by oxidative damage and apoptosis. Similar results were obtained in human proximal tubular (HK-2) cells damaged by AAI. In the presence of a low concentration of the APE1 inhibitor E3330, expression of Nrf2 and HO-1 proteins in HK-2 cells was decreased and AAI-induced apoptosis was aggravated. Overexpression of APE1 in HK-2 cells promoted the expression of Nrf2 and HO-1, and alleviated apoptosis and renal injury induced by AAI. The collective findings demonstrate that AAI can inhibit the induction of oxidative stress and apoptosis by the APE1/Nrf2/HO-1 axis, leading to AAI renal injury. Targeting APE1 may be an effective therapeutic strategy to treat AA nephrotoxicity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/15376516.2023.2250429 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Inhibition of ferroptosis alleviates acute kidney injury caused by diquat in zebrafish].
Nan Fang Yi Ke Da Xue Xue Bao
August 2025
Key Laboratory of Occupational Environment and Health, Guangzhou Twelfth People's Hospital, Guangzhou 510620, China.
Objectives: To investigate the role of ferroptosis in diquat-induced acute kidney injury (AKI) and its molecular mechanisms.
Methods: Transgenic zebrafish models with Tg (Eco.Tshb:EGFP) labeling of the renal tubules and Tg (lyz:dsRed2) labeling of the neutrophils were both divided into control group, gentamicin (positive control) group, diquat poisoning group, ferroptosis inhibitor group.
[ Granules ameliorate renal damage in MRL/lpr mice by inhibiting the MyD88/NF-κB pathway].
Nan Fang Yi Ke Da Xue Xue Bao
August 2025
First Affiliated Hospital of Anhui University of Chinese Medicine.
Objectives: To investigate the mechanism of (QJZ) for ameliorating renal damage in MRL/lpr mice.
Methods: With 6 female C57BL/6 mice as the normal control group, 30 female MRL/lpr mice were randomized into model group, QJZ treatment groups at low, moderate and high doses, and prednisone treatment group (6). After 8 weeks of treatment, the mice were examined for 24-h urine protein, creatinine and albumin levels, serum levels of IgG, complement 3 (C3), C4, anti-dsDNA, interferon γ (IFN‑γ) and interleukin 17 (IL-17).
Exploring the protective role of DDIT4/mTOR in podocyte integrity through macrophage polarization in diabetic kidney disease.
Ren Fail
December 2025
Department of Nephrology, China-Japan Friendship Hospital, Beijing, China.
Objectives: In this study, we explored the mechanism by which DDIT4 influences the polarization phenotypic transformation of macrophages and inflammation through the regulation of mTOR signaling pathway, providing a new mechanism and target for the treatment of diabetic nephropathy.
Methods: The degree of inflammation and injury in renal tissues of diabetic kidney disease (DKD) animal model was evaluated using biochemical assays, renal pathology examinations, and Western blot tests. Podocytes and macrophages were isolated from renal tissues to observe the extent of podocyte injury and the quantity and polarization phenotype of macrophage infiltration.
Advanced Three-Dimensional Cell Culture Models for Investigating Enterohaemorrhagic Pathogenesis and Shiga Toxin-Mediated Injury.
J Microbiol Biotechnol
September 2025
Environmental Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Enterohemorrhagic (EHEC), a pathotype within the Shiga toxin-producing (STEC) group, is a major etiological agent of severe gastrointestinal illness and life-threatening sequelae, including hemolytic uremic syndrome. Although insights into EHEC pathogenesis have been gained through traditional 2D cell culture systems and animal models, these platforms are limited in their ability to recapitulate human-specific physiological responses and tissue-level interactions. Recent progress in three-dimensional (3D) cell culture systems, such as spheroids, organoids, and organ-on-a-chip (OoC) technologies, has enabled more physiologically relevant models for investigating host-pathogen dynamics.
View Article and Find Full Text PDFQuantitative Susceptibility Mapping for Evaluating Renal Functional Injury in Chronic Kidney Disease Patients: A Pilot Study.
NMR Biomed
October 2025
Department of Radiology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
Chronic kidney disease (CKD) is an increasing global health problem, resulting in gradual loss of renal function and irreversible renal injury. The noninvasive detection, monitoring, and timely intervention of CKD might benefit the patients' prognosis. This study aims to assess renal functional injury in CKD patients by using magnetic resonance imaging (MRI) of quantitative susceptibility mapping (QSM).
View Article and Find Full Text PDF