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Article Abstract
Objective: To establish HL-60 cells and adriamycin resistant HL-60 cells (H-60/ADR) in which the expression of homologous box gene 1 () was inhibited, and investigate the effect of inhibiting the expression of on the drug resistance.
Methods: Lentivirus was used to transfect HL-60 and HL-60/ADR cells, and the cell lines stably inhibiting the expression of were screened by puromycin. CCK-8 assay was used to detect the proliferation ability of cells in each group, apoptosis kit was used to detect the cell apoptosis, and real-time quantitative PCR was used to detect the expression level of drug-resistant related genes.
Results: HL-60 and HL-60/ADR stably transfected cell lines with down-regulation of expression were successfully constructed. Compared with control group, the inhibition of expression significantly inhibited the proliferation of HL-60 and HL-60/ADR cells ( <0.05), increased the apoptosis rate ( <0.05), and the sensitivity of cells to adriamycin increased after inhibition of expression.
Conclusion: Inhibition of expression can improve cell sensitivity to adriamycin, and its role in reversing drug resistance may be related to the promotion of apoptosis gene expression.
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| http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2023.04.017 | DOI Listing |
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