Article Synopsis
- The conversion of lignocellulosic materials into fermentable sugars for biofuel production is typically inefficient, often leading to growth issues when targeting lignin biosynthesis.
- Researchers found that expressing Pag-miR408 in poplar trees improves saccharification without needing acid treatment and even boosts plant growth.
- Pag-miR408 affects lignin production by targeting specific enzymes, resulting in better accessibility of plant cell walls to conversion processes, which could enhance biomass yield for bioenergy.
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Article Abstract
The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354043 | PMC |
| http://dx.doi.org/10.1038/s41467-023-39930-3 | DOI Listing |
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