Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Laboratory-based studies on microbial Fe(II) oxidation are commonly performed for 5-10 days in small volumes with high substrate concentrations, resulting in geochemical gradients and volumetric effects caused by sampling. We used a chemostat to enable uninterrupted supply of medium and investigated autotrophic nitrate-reducing Fe(II)-oxidizing culture KS for 24 days. We analysed Fe- and N-speciation, cell-mineral associations, and the identity of minerals. Results were compared to batch systems (50 and 700 mL-static/shaken). The Fe(II) oxidation rate was highest in the chemostat with 7.57 mM Fe(II) d , while the extent of oxidation was similar to the other experimental setups (average oxidation of 92% of all Fe(II)). Short-range ordered Fe(III) phases, presumably ferrihydrite, precipitated and later goethite was detected in the chemostat. The 1 mM solid phase Fe(II) remained in the chemostat, up to 15 μM of reactive nitrite was measured, and 42% of visualized cells were partially or completely mineral-encrusted, likely caused by abiotic oxidation of Fe(II) by nitrite. Despite (partial) encrustation, cells were still viable. Our results show that even with similar oxidation rates as in batch cultures, cultivating Fe(II)-oxidizing microorganisms under continuous conditions reveals the importance of reactive nitrogen intermediates on Fe(II) oxidation, mineral formation and cell-mineral interactions.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10316368 | PMC |
| http://dx.doi.org/10.1111/1758-2229.13149 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Accelerating iron redox cycling via acetate modification: a ligand engineering for sustainable fenton-like oxidation.
Water Res
September 2025
State Key Laboratory of Soil Pollution Control and Safety, Department of Environmental Science, Zhejiang University, Hangzhou 310058, China; Future Environment Laboratory, Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314100, China. Electronic address:
Accelerating the rate-limiting surface Fe(III)/Fe(II) redox cycling is pivotal for efficient iron-mediated Fenton-like decontamination, yet conventional reductants (e.g., toxic hydroxylamine, thiosulfate) suffer from secondary toxicity, self-quenching, and heavy metal leaching.
View Article and Find Full Text PDFMethionine-based insights into C-S-Fe-P transformations in anaerobic co-digestion of sludge containing iron-phosphorus compounds.
Water Res
August 2025
State Key Laboratory of Water Pollution Control and Green Resource Recycling, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:
Anaerobic co-digestion of sulfur-containing organic wastes with waste-activated sludge containing iron-phosphorus compounds (FePs) was recently suggested as an environment-friendly strategy to promote phosphate release, energy recovery, and hydrogen sulfide (HS) control. Nevertheless, the mechanistic coupling between FePs speciation and the concurrent transformation of carbon, sulfur, iron, and phosphorus within this system remains to be fully elucidated. To address this knowledge gap, methionine, a typical hydrolysis product of sulfur-containing organics, and five FePs prevalent in sludge (ferric-phosphate tetrahydrate (FePO⋅4HO), ferric-phosphate dihydrate (FePO⋅2HO), vivianite (Fe(PO)·8HO), phosphate coprecipitated with Fe(III) (COP-P), and phosphate adsorption on hydrous ferric oxide (HFO-P)) were selected to elucidate C-S-Fe-P transformations in this study.
View Article and Find Full Text PDFA role for Dps ferritin activity in long-term survival of .
Microbiol Spectr
September 2025
Department of Biological Sciences, Molecular and Computational Biology Section, University of Southern California, Los Angeles, California, USA.
expresses three ferritins that store acquired iron by oxidizing soluble Fe(II) to insoluble Fe(III), which can accumulate and later be utilized in cellular processes. Although bacterioferritin (Bfr) and ferritin (FtnA) sequester more Fe(III) atoms per multimeric complex, the abundance of the DNA-binding protein from starved cells (Dps), coupled with its preference for hydrogen peroxide as an oxidant in its ferroxidase activity, makes it a fundamental component in iron homeostasis and long-term stationary phase (LTSP) survival. To investigate the temporal role and mechanisms of action of Dps in parallel with the other ferritins, growth yield, survival, competitive fitness, and siderophore assays were performed under different conditions of iron availability.
View Article and Find Full Text PDFA novel technology based on aeration-coupled molecular oxygen activation for in-situ sustainable groundwater remediation: Mechanisms and feasibility assessment.
Water Res
August 2025
Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China; Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun 130021, China; National and Local Joint Engineering Laboratory for Petrochemical
In-situ chemical oxidation (ISCO) based on Fe(II)-activated O is a promising approach for groundwater remediation; however, its efficiency is often limited by the low oxygen content in subsurface environments and insufficient generation of reactive oxygen species (ROS). In this study, a novel ISCO technology was developed by integrating a tripolyphosphate (TPP)-enhanced Fe(II)/O advanced oxidation system with aeration to promote ROS generation and enhance remediation performance. Two-dimensional sand tank experiments were conducted to evaluate the effectiveness of this approach in remediating p-Nitrophenol (PNP) contaminated groundwater.
View Article and Find Full Text PDFEvidence human FTO catalyses hydroxylation of N6-methyladenosine without direct formation of a demethylated product contrasting with ALKBH5/2/3 and bacterial AlkB.
Nucleic Acids Res
August 2025
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.
N 6-Methyladenosine (m6A) is a prevalent post-transcriptional modification in eukaryotic messenger RNA. Two cancer-linked human Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenases, the fat mass and obesity associated-protein (FTO), and AlkB human homolog 5 (ALKBH5) catalyse m6A methyl group oxidation. While ALKBH5 has consistently been reported to catalyse m6A demethylation, there are conflicting reports concerning the FTO products.
View Article and Find Full Text PDF