Production of (10,11)-(-)--Pyriculol and Its HPLC Quantification in Liquid Cultures of , a Potential Mycoherbicide for the Control of Buffelgrass ().

(10,11)-(-)--pyriculol is a phytotoxic metabolite produced by , a fungus identified as a foliar pathogen on the invasive weed species buffelgrass () in North America. The effective control of buffelgrass has not yet been achieved, and there is a need to develop effective and green solutions. Herbicides based on natural products and the use of phytopathogenic organisms could provide the most suitable tools for the control of weeds such as buffelgrass. Thus, one of the most relevant points to study about potential suitable phytotoxins such as (10,11)-(-)--pyriculol is its production on a large scale, either by isolation from fungal fermentations or by synthesis. For these purposes, rapid and sensitive methods for the quantification of (10,11)-(-)--pyriculol in complex mixtures are required. In this study, a high-pressure liquid chromatography (HPLC) method for its quantification was developed and applied to organic extracts from twelve isolates obtained from diseased buffelgrass leaves and grown in potato dextrose broth (PDB) liquid cultures. The analysis proved that the production of (10,11)-(-)--pyriculol is fungal-isolate dependent and strongly correlated with phytotoxic activity, shown by the organic extracts in a buffelgrass radicle elongation test. The HPLC method reported herein allowed us to select the best strain for the production of (10,11)-(-)--pyriculol and could be useful for selecting the best cultural conditions for its mass production, providing a tool for the use of this promising metabolite as a new bioherbicide for the control of buffelgrass.