98%
921
2 minutes
20
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1021/acs.nanolett.2c00593 | DOI Listing |
Biosens Bioelectron
December 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China. Electronic address:
Herein, through the cascade integration of the RCA and HCR reactions, an innovative linear multi-shear site DNA walker (LMDW) with high walking efficiency was developed to fabricate an electrochemical biosensing platform for detecting microRNA-21 (miRNA-21) associated with breast cancer. Impressively, compared with conventional single-shear site DNA walkers with poor walking efficiency, the LMDW obtained from the RCA-confined HCR reaction can accommodate a considerable number of functional domains, increasing the shear efficiency to the hairpin probe with the beacon material, thereby consequently enhancing the reaction rate and sensitivity of the biosensor. Following validation, the probe acquisition time down to just 20 min, far less than traditional single-shear site DNA walkers and the detection limit was 0.
View Article and Find Full Text PDFBiosens Bioelectron
November 2025
The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Science, Nankai University, Weijin Road No.94, Tianjin, 300071, PR China. Electronic address:
With its high porosity and tunable properties, metal-organic frameworks (MOFs) serve as nano-cornucopias, unlocking limitless possibilities in biosensing applications. Herein, we designed an ultrasensitive biosensor leveraging MOFs as a multifunctional nano-platform to implement a synergistic self-calibration strategy for the ultra-accurate quantification of breast cancer-related human epidermal growth factor receptor-2 (HER-2). This approach integrates (1) ratiometric electrochemistry for built-in correction to minimize non-specific interference and (2) dual-modal electrochemical and colorimetric detection for enhanced accuracy through cross-validation.
View Article and Find Full Text PDFNat Commun
July 2025
Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano and Soft Materials (FUNSOM), Soochow University, Suzhou, 215123, China.