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Two papers published in June 2021 used a two-photon microscope or one-photon miniature microscope to interrogate the motor cortex in behaving macaque monkeys. The imaging was performed over several months, and the direction of natural arm reaching was decoded from the population activity.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329299 | PMC |
http://dx.doi.org/10.1038/s41467-021-24988-8 | DOI Listing |
The perivascular space (PVS) plays a crucial role in facilitating the clearance of waste products and the exchange of cerebrospinal fluid and interstitial fluid in the central nervous system. While optical imaging methods identify the glymphatic transport of fluorescent tracers through PVS of surface-diving arteries, their limited depth penetration impedes the study of glymphatic dynamics in deep brain regions. In this study, we introduced a novel high-resolution dynamic contrast-enhanced MRI mapping approach based on single-vessel multi-gradient-echo methods.
View Article and Find Full Text PDFNat Methods
April 2023
National Biomedical Imaging Center, State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
In deep-tissue multiphoton microscopy, diffusion and scattering of fluorescent photons, rather than ballistic emanation from the focal point, have been a confounding factor. Here we report on a 2.17-g miniature three-photon microscope (m3PM) with a configuration that maximizes fluorescence collection when imaging in highly scattering regimes.
View Article and Find Full Text PDFBiomaterials
October 2022
Interdisciplinary Institute of Neuroscience and Technology (ZIINT), Department of Anesthesiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310020, China; MOE Frontier Science Center for Brain Research and Brain Machine Integration, Zhejiang University, Hangz
Multiphoton microscopy has been a powerful tool in brain research, three-photon fluorescence microscopy is increasingly becoming an emerging technique for neurological research of the cortex in depth. Nonhuman primates play important roles in the study of brain science because of their neural and vascular similarity to humans. However, there are few research results of three-photon fluorescence microscopy on the brain of nonhuman primates due to the lack of optimized imaging systems and excellent fluorescent probes.
View Article and Find Full Text PDFNat Biotechnol
November 2022
Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.
High-resolution optical imaging deep in tissues is challenging because of optical aberrations and scattering of light caused by the complex structure of living matter. Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering induced by specimens and recovers subcellular resolution at depth.
View Article and Find Full Text PDFExp Eye Res
August 2022
Molecular Aging and Development Laboratory, Boston University School of Medicine, Boston, MA, 02118, USA; Boston University Alzheimer's Disease Research Center, Boston University School of Medicine, 72 East Concord Street, B-7800 Boston, MA, 02118, USA. Electronic address: