Optical deep-cortex exploration in behaving rhesus macaques.

Nat Commun

Department of Physiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Published: August 2021


Category Ranking

98%

Total Visits

921

Avg Visit Duration

2 minutes

Citations

20

Article Abstract

Two papers published in June 2021 used a two-photon microscope or one-photon miniature microscope to interrogate the motor cortex in behaving macaque monkeys. The imaging was performed over several months, and the direction of natural arm reaching was decoded from the population activity.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329299PMC
http://dx.doi.org/10.1038/s41467-021-24988-8DOI Listing

Publication Analysis

Top Keywords

optical deep-cortex
4
deep-cortex exploration
4
exploration behaving
4
behaving rhesus
4
rhesus macaques
4
macaques papers
4
papers published
4
published june
4
june 2021
4
2021 two-photon
4

Similar Publications

The perivascular space (PVS) plays a crucial role in facilitating the clearance of waste products and the exchange of cerebrospinal fluid and interstitial fluid in the central nervous system. While optical imaging methods identify the glymphatic transport of fluorescent tracers through PVS of surface-diving arteries, their limited depth penetration impedes the study of glymphatic dynamics in deep brain regions. In this study, we introduced a novel high-resolution dynamic contrast-enhanced MRI mapping approach based on single-vessel multi-gradient-echo methods.

View Article and Find Full Text PDF

Miniature three-photon microscopy maximized for scattered fluorescence collection.

Nat Methods

April 2023

National Biomedical Imaging Center, State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.

In deep-tissue multiphoton microscopy, diffusion and scattering of fluorescent photons, rather than ballistic emanation from the focal point, have been a confounding factor. Here we report on a 2.17-g miniature three-photon microscope (m3PM) with a configuration that maximizes fluorescence collection when imaging in highly scattering regimes.

View Article and Find Full Text PDF

Large-depth three-photon fluorescence microscopy imaging of cortical microvasculature on nonhuman primates with bright AIE probe In vivo.

Biomaterials

October 2022

Interdisciplinary Institute of Neuroscience and Technology (ZIINT), Department of Anesthesiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310020, China; MOE Frontier Science Center for Brain Research and Brain Machine Integration, Zhejiang University, Hangz

Multiphoton microscopy has been a powerful tool in brain research, three-photon fluorescence microscopy is increasingly becoming an emerging technique for neurological research of the cortex in depth. Nonhuman primates play important roles in the study of brain science because of their neural and vascular similarity to humans. However, there are few research results of three-photon fluorescence microscopy on the brain of nonhuman primates due to the lack of optimized imaging systems and excellent fluorescent probes.

View Article and Find Full Text PDF

High-resolution optical imaging deep in tissues is challenging because of optical aberrations and scattering of light caused by the complex structure of living matter. Here we present an adaptive optics three-photon microscope based on analog lock-in phase detection for focus sensing and shaping (ALPHA-FSS). ALPHA-FSS accurately measures and effectively compensates for both aberrations and scattering induced by specimens and recovers subcellular resolution at depth.

View Article and Find Full Text PDF

Alzheimer's disease amyloid-β pathology in the lens of the eye.

Exp Eye Res

August 2022

Molecular Aging and Development Laboratory, Boston University School of Medicine, Boston, MA, 02118, USA; Boston University Alzheimer's Disease Research Center, Boston University School of Medicine, 72 East Concord Street, B-7800 Boston, MA, 02118, USA. Electronic address:

Article Synopsis
  • Alzheimer’s disease (AD) causes harmful buildup of a protein called amyloid-β in the brain, which can start many years before symptoms show.
  • Research found similar amyloid-β deposits in the lenses of people's eyes with AD, which could cause special cataracts not seen in normal aging.
  • A study showed that the traits linked to amyloid-β in the eyes could predict future Alzheimer's problems in the brain and help identify a new risk factor related to AD.
View Article and Find Full Text PDF