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The likelihood of finding intact cellular structures on the surface or in the near subsurface of the martian regolith is slim, due in part to the intense bombardment of the surface by ionizing radiation from outer space. Given that this radiation is predicted to be so intense that it would render a living cell inactive within minutes, it is logical to search for evidence of microbial life by looking for molecules produced by the breakdown of cellular matter. This "pool" of molecules, known as biomarkers, consists of a range of species with various functionalities that make them likely to interact with minerals in the martian regolith. Raman spectroscopy, a molecularly specific analysis method utilized for detecting organic biomarkers among inorganic geomaterials, suffers from low signal intensity when the concentration of organics is as low as it appears to be on the martian surface. This article describes the utility of a surface-enhanced Raman spectroscopy (SERS) method used to detect extremely low levels of biomarkers that were passively adhered to mineral surfaces in a method that represents how this interaction would take place in a natural environment on Mars. The methodology showed promise for the detection of multiple classes of biomarkers.
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http://dx.doi.org/10.1089/ast.2020.2278 | DOI Listing |
Anal Chem
September 2025
Institute of Digitized Medicine and Intelligent Technology, Wenzhou Medical University, Wenzhou 325000, P. R. China.
Surface-enhanced Raman spectroscopy (SERS) has shown potential for early disease diagnosis via urinary metabolomics, but still faces challenges in achieving stable hot spots and processing complex clinical data. In this study, the preparation of chiral gold nanostars with precisely controllable branch size, number, and sharpness was realized by investigating the effects of l-GSH and CTA ( indicates halides) on site occupancy, reduction rate, and selective adsorption on crystal facets. Raman spectroscopic characterization using rhodamine 6G (R6G) as a reporter molecule revealed that nanoparticles with fewer branches, larger branch bases, and smoother surfaces exhibited excellent SERS activity, with an analytical enhancement factor (AEF) of 5.
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September 2025
Gynaecology and Obstetrics, The Second Affiliated Hospital of Harbin Medical University, Harbin Medical University, Heilongjiang 150081, PR China. Electronic address:
Single-cell surface-enhanced Raman scattering (SERS) has emerged as a powerful tool for precision medicine owing to its label-free detection, ultrasensitivity, and unique molecular fingerprinting. Unlike conventional bulk analysis, it enables detailed characterization of cellular heterogeneity, with particular promise in circulating tumor cell (CTC) identification, tumor microenvironment (TME) metabolic profiling, subcellular imaging, and drug sensitivity assessment. Coupled with microfluidic droplet systems, SERS supports high-throughput single-cell analysis and multiparametric screening, while integration with complementary modalities such as fluorescence microscopy and mass spectrometry enhances temporal and spatial resolution for monitoring live cells.
View Article and Find Full Text PDFCell Rep Med
August 2025
Center for Biomedical-photonics and Molecular Imaging, Advanced Diagnostic-Therapy Technology and Equipment Key Laboratory of Higher Education Institutions in Shaanxi Province, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi 710126, China; Engineering Research Center of Mole
Rapid identification and accurate diagnosis are critical for individuals with acute leukemia (AL). Here, we propose a combined deep learning and surface-enhanced Raman scattering (DL-SERS) classification strategy to achieve rapid and sensitive identification of AL with various subtypes and genetic abnormalities. More than 390 of cerebrospinal fluid (CSF) samples are collected as targets, encompassing healthy control, AL patients, and individuals with other diseases.
View Article and Find Full Text PDFVirology
September 2025
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China. Electronic address:
Colloidal gold technology has revolutionized viral diagnostics through its rapid, cost-effective, and user-friendly applications, particularly in point-of-care testing (POCT). This review synthesizes recent advancements, focusing on its role in detecting respiratory viruses, hepatitis viruses, and emerging pathogens. The technology leverages the unique optical and physicochemical properties of gold nanoparticles (AuNPs), including localized surface plasmon resonance (LSPR) and high surface-to-volume ratios, to achieve rapid antigen-antibody recognition with visual readouts within 15 min.
View Article and Find Full Text PDFAnal Chem
September 2025
State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, College of Energy, Discipline of Intelligent Instrument and Equipment, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361
Rolling circle amplification (RCA) has revolutionized nucleic acid detection owing to its isothermal simplicity. However, over two decades of clinical application have been hampered by off-target amplification and incompatibility with double-stranded DNA (dsDNA). Herein, a strategy, specifically cleavage of rationally designed DNA/RNA chimeric hairpin preprimer by dsDNA-targeted CRISPR/Cas12a to rlease ssRNA for initiating RCA (SCOPE-RCA), is proposed for nucleic acid identification of African swine fever virus (ASFV).
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