Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Accumulating evidences have shown that long noncoding RNAs (lncRNAs) play key roles in many diseases, including cancer. Several studies reported that MCM3AP antisense RNA 1 (MCM3AP-AS1) was associated with the tumorigenesis and progression. However, the specific function and mechanism of MCM3AP-AS1 in colorectal cancer (CRC) have not been fully understood.
Methods: The expression of MCM3AP-AS1 was detected by quantitative reverse transcription PCR (RT-qPCR) in CRC tissues and matched noncancerous tissues (NCTs). CCK-8 assay, colony formation assay, transwell assay, xenograft and lung metastasis mouse models were used to examine the tumor-promoting function of MCM3AP-AS1 in vitro and in vivo. The binding relationship between MCM3AP-AS1, miR-193a-5p and sentrin-specific peptidase 1 (SENP1) were screened and identified by databases, RT-qPCR, dual luciferase reporter assay and western blot.
Results: In the present study, we got that the expression of MCM3AP-AS1 was higher in CRC tissues than in paired NCTs, and increased MCM3AP-AS1 expression was associated with adverse outcomes in CRC patients. Functional experiments in vitro revealed that silencing of MCM3AP-AS1 could inhibit the proliferation, colony formation, migratory, and invasive abilities of CRC cells. The mouse models of xenograft and lung metastasis further confirmed that in vivo silencing MCM3AP-AS1 could significantly inhibit the growth and metastasis of CRC. Further mechanism studies indicated that MCM3AP-AS1 could sponge miR-193a-5p and inhibit the activity of it. What is more, SENP1 was proved to be a novel target of miR-193a-5p and could be upregulated by MCM3AP-AS1. At last, we observed that SENP1 overexpression in CRC tissues was closely related to unfavorable prognosis.
Conclusion: Taken together, we identified in CRC the MCM3AP-AS1/miR-193a-5p/SENP1 regulatory axis, which affords a therapeutic possibility for CRC.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982620 | PMC |
| http://dx.doi.org/10.1002/cam4.3830 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prognostic Value of MCM3AP-AS1 in Glioma and its Regulatory Effect on Tumor Progression.
Neurochem Res
September 2025
Department of Neurosurgery, Zibo Central Hospital, No. 54, Gongqingtuan West Road, Zhangdian District, Zibo, 255000, China.
Long non-coding RNAs (lncRNAs) have emerged as promising cancer biomarkers due to their stability and detectability. This study aimed to investigate the clinical significance and molecular mechanisms of lncRNA MCA3AP-AS1 in glioma. This study collected the clinical data from 177 glioma patients, and the expression of MCM3AP-AS1 was measured in glioma tissues and cell lines.
View Article and Find Full Text PDFSilencing lncRNA MCM3AP-AS1 protects against cisplatin-induced cell death in A549 lung cancer cells.
Mol Biol Rep
July 2025
Respiratory Diseases and Respiratory Surgery Research and Practice Center, Gaziantep University, Gaziantep, Turkey.
Background: Lung cancer remains the leading cause of cancer-related mortality worldwide, largely due to the development of resistance to standard chemotherapeutic agents such as cisplatin. Recent evidence has highlighted the regulatory role of long non-coding RNAs (lncRNAs) in mediating drug resistance mechanisms. In this study, we aimed to reveal the role of MCM3AP-AS1 in cisplatin-induced cell death in lung cancer cells.
View Article and Find Full Text PDFLncRNA MCM3AP-AS1 protects against cerebral ischemia-reperfusion injury via targeting miR-27b-3p.
Neurol Res
July 2025
Department of Neurosurgery, Nanjing Medical University Affiliated Changzhou No. 2 People's Hospital, Changzhou, China.
Objectives: Cerebral ischemia-reperfusion (CI/R) injury is a significant hurdle in ischemic stroke treatment. Substantial evidence indicates that long non-coding RNAs (lncRNAs) are implicated in CI/R injury. Here, we explore the function of lncRNA MCM3AP-AS1 in CI/R injury.
View Article and Find Full Text PDFExpression and significance of lncRNAs derived from PBMC in rheumatoid arthritis.
Front Immunol
May 2025
Department of Rheumatology and Immunology, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
Background: Long non-coding RNAs (lncRNAs) are gaining recognition for their critical involvement in diverse autoimmune disorders. Nevertheless, reseach investigating the role of lncRNAs in rheumatoid arthritis (RA) is relatively scarce.
Methods: Comprehensive transcriptome sequencing was executed to acquire a lncRNA expression pattern in peripheral blood mononuclear cells (PBMC) of RA.
Overexpression of Long Non-coding RNAs and Predict Poor Prognosis in Patients with Gastric Adenocarcinoma.
Adv Biomed Res
April 2024
Baqiyatallah Research Center for Gastroenterology and Liver Disease, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Background: LINC00092 and MCM3AP-AS1 long non-coding RNAs (LncRNAs) play significant roles in the development and pathogenesis of many cancers. However, their expression levels and prognostic values were not evaluated in human gastric adenocarcinoma (GAC). Therefore, the present study aimed to investigate the clinico-pathological correlations of LINC00092 and MCM3AP-AS1, LncRNAs expression in GAC, and evaluate their prognostic values.
View Article and Find Full Text PDF