Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
A fluorescent assay for total antioxidant capacity (TAC) detection based on the light-responsive oxidase-like activity of bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) has been developed. Thiamine (TH) as the peroxidase substrate usually works at alkaline conditions and thus limits its practical applications. Here, by utilization the light-responsive oxidase-like activity of BSA-AuNCs, TH is oxidized to fluorescent thiochrome under neutral condition in two minutes due to the single oxygen generated by BSA-AuNCs upon light irradiation. After the introduction of antioxidants into the BSA-AuNCs-TH system, the formation of thiochrome is inhibited resulting in the fluorescence decrease. On the basis of the above facts, BSA-AuNCs-TH-based assay has been fabricated and applied successfully to detect antioxidants and the TAC of vitamin C tablets as well as some commercial fruit juice with satisfied results. This work may provide novel insights into developing light-responsive nanozymes-based fluorescent assays.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jhazmat.2021.125106 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Non-Plasmonic Oxidase-Like Gold Nanocatalysts on Hydrogel Beads for Broad-Spectrum Water Decontamination.
Langmuir
September 2025
Department of Chemistry, Indian Institute of Technology Guwahati, North Guwahati, Kamrup, Assam 781039, India.
The efficient and sustainable remediation of contaminated water calls for catalytic systems that must clean broadly, endure widely, and last repeatedly. In this regard, we report the development of sulfonate-functionalized core-shell hydrogel beads embedded with synthesized gold nanoparticles (AuNPs) that exhibit intrinsic oxidase-like activity without requiring external light or chemical oxidants. The sulfonate ligands modulate the surface electronic environment of the AuNPs, facilitating singlet oxygen generation via a nonplasmonic, radiationless mechanism.
View Article and Find Full Text PDFDual-mode nanozyme-hydrogel platform for on-site multi-target detection of biomarkers and hazardous substances.
Mikrochim Acta
September 2025
Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe District, Shenyang, 110016, Liaoning, People's Republic of China.
A novel dual-mode sensing system integrating a magnetic core-shell CuFeO/Cu/MnO nanozyme with a stimuli-responsive agarose-deep eutectic solvent hydrogel (DES-Aga) is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (K = 0.14 mM) and high catalytic efficiency (V = 1.
View Article and Find Full Text PDFKinetic and Mechanistic Discrepancies of Single/Dual-Atom Nanozymes Drive a Triple-Channel Sensing Array for Machine Learning-Assisted Antioxidant Discrimination.
Anal Chem
September 2025
Anhui Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, P.R. China.
Current colorimetric sensing arrays for antioxidant detection often struggle with discrimination due to cross-reactive signals from individual nanozymes. These signals are typically modulated by external factors such as pH or chromogenic substrates, offering limited kinetic and mechanistic diversity. To overcome this, we present a novel triple-channel colorimetric sensing array utilizing two distinct single-atom nanozymes (Cu SA and Fe SA) and one dual-atom nanozyme (CuFe DA).
View Article and Find Full Text PDFIdentifying the Oxidase-like Behavior of N-Doped Carbon Nanozymes via Postsynthetic Modification for Colorimetric Sensing.
Anal Chem
September 2025
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
N-doped carbon nanomaterials (NCMs) have attracted significant interest as metal-free nanozymes for sensing due to their exceptional stability and biocompatibility. However, the controversial active sites and catalytic pathways severely hinder the application of NCM-based nanozymes. Here, postsynthetic modification methods have been developed to study the catalytic mechanism, including selective deactivation, chemical grafting, and surface doping.
View Article and Find Full Text PDFA multifunctional oxidative stress amplifier for synergistic disruption of redox homeostasis and enhanced cancer therapy.
J Colloid Interface Sci
August 2025
College of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang, 050017, China; National Key Laboratory of New Pharmaceutical Preparations and Excipients, Hebei Medical University, Shijiazhuang 050017, China. Electronic address:
Emerging nanomedicines that target and disrupt redox homeostasis present a compelling yet technically demanding strategy for cancer therapy. Herein, a multifunctional oxidative stress amplifier, denoted as C-COF@MnO-BSA-FA/Ce6 (CMBFC), was engineered to disrupt redox homeostasis through synergistic mechanisms precisely. The nanoplatform was constructed with a core of N-doped carbon nanospheres derived from covalent organic frameworks (C-COF), which was then coated by an in situ mineralized MnO layer.
View Article and Find Full Text PDF