Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The fluorescent probes with good water-solubility, long-wavelength emission and large Stokes shift are greatly desirable for in vivo detection. Herein, we designed a novel 1,8-naphthalimide-based near-infrared (NIR) optical and fluorescent probe (NTC) for sensing cysteine (Cys). Using acrylate as the recognition site, the probe demonstrated high selectivity and sensitivity for Cys with a low detection limit (0.093 μM) in aqueous buffer solution due to the excellent water-solubility. Upon the reaction with Cys, the recovery of intramolecular charge transfer (ICT) in the probe led to about 40-fold fluorescence enhancement. Furthermore, the reaction result was investigated by H NMR, and HRMS analyses, and the sensing mechanism was validated by quantum calculations. Finally, NTC was applied to image exogenous and endogenous Cys in HeLa cells and zebrafish selectively, implying that the probe possessed great potential application in biological fluorescence sensing.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.saa.2020.119030 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Histone Methyltransferase EHMT2 Promotes the Progression of Breast Ductal Carcinoma by Regulating the Hippo Pathway.
J Environ Pathol Toxicol Oncol
September 2025
Department of Clinical Laboratory Medicine, Fujian Medical University, Fuzhou, China.
Invasive ductal carcinoma (IDC) is a major type of breast cancer. The utilization of inhibitors targeting histone methyltransferases introduces novel therapeutic avenues for the treatment of cancer. Immunohistochemistry, Western blot, and reverse transcription quantitative polymerase chain reaction experiments were applied to assess the levels of EHMT2 in IDC and adjacent tissues.
View Article and Find Full Text PDFAn ultrasensitive biosensor for H1N1 virus coupled with 3D spherical DNA nanostructure and CRISPR-Cas12a.
Spectrochim Acta A Mol Biomol Spectrosc
September 2025
Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun 130122, China. Electronic address:
To achieve ultrasensitive and real-time detection of the H1N1 influenza virus, this study designed a nucleic acid-free fluorescent biosensor based on 3D spherical DNA nanostructure and CRISPR/Cas12a (3D-SDNC). The biosensor constructs a rigid 3D nano-framework via self-assembly of six oligonucleotide chains, with H1N1-specific nucleic acid aptamers and Cas12a activator strands strategically positioned at multi-spined vertices for precise spatial coupling between viral recognition and signal transduction. Upon aptamer-virus binding, the induced conformational change liberates the activator strand, thereby activating the trans-cleavage activity of the Cas12a/crRNA complex to efficiently cleave the HEX/BHQ1 double-labeled fluorescent probe and initiate cascade signal amplification.
View Article and Find Full Text PDFTropism and Retinal Transduction Efficiency of Adeno-Associated Virus Serotypes in Mice.
Invest Ophthalmol Vis Sci
September 2025
Department of Ophthalmology, Dean McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States.
Purpose: Adeno-associated viruses (AAVs) have become the preferred vector for gene therapy in ophthalmology. However, requirements for specific cell surface receptors limit AAV-mediated retinal cell transduction efficiency. This led to the need to engineer novel AAV vectors for widespread retinal transduction and transgene expression.
View Article and Find Full Text PDFFlexible, Transparent, and Microfluidic-Compatible Wafer-Scale Metamaterial Sheets for Dual SEF and SERS Sensing.
ACS Appl Mater Interfaces
September 2025
National Key Laboratory of Advanced Micro and Nano Manufacture Technology, Shanghai Jiao Tong University, Shanghai 200240, China.
Integrating surface-enhanced fluorescence (SEF) and surface-enhanced Raman spectroscopy (SERS) into a single probe is a natural step forward for plasmon-enhanced spectroscopy (PES), as SEF enables enhanced fluorescent imaging for fast screening of targets, while SERS allows ultrasensitive trace molecular characterization with specificity. However, many challenges remain, e.g.
View Article and Find Full Text PDFA fluorescent probe with a vanillin-pyridine-imidazole core structure for carboxylesterase detection in macrophage polarization during bone homeostasis.
Front Chem
August 2025
Department of Food Science and Nutrition, Hallym University, Chuncheon, Republic of Korea.
In this work, a fluorescent probe, VanPI-CarE, with a vanillin-pyridine-imidazole core structure was developed for carboxylesterase (CarE) detection in macrophage polarization during bone homeostasis. The probe responded to CarE with a distinct fluorescence reporting signal at 490 nm upon excitation at 355 nm. Tests in solution showed the advantages of VanPI-CarE, including high sensitivity, excellent stability under various working conditions, high selectivity, and low cytotoxicity.
View Article and Find Full Text PDF