Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
High-throughput assays are a common strategy for the identification of compounds able to modulate a certain cellular activity. Here, we show an automatized analysis platform for the quantification of nuclear foci as inhibitory effect of compounds on a target protein labeled by fluorescent antibodies. Our experience led us to a fast analysis platform that combines cell-based assays, high-content screening, and confocal microscopy, with an automatic and user-friendly statistical analysis of plate-based assays including positive and negative controls, able to identify inhibitory effect of compounds tested together with the Z-prime and Window of individual plate-based assays to assess the reliability of the results. The platform integrates a web-based tool implemented in Pipeline Pilot and R, and allows computing the inhibition values of different parameters obtained from the high-content screening and confocal microscopy analysis. This facilitates the exploration of the results using the different parameters, providing information at different levels as the number of foci observed, the sum of intensity of foci, area of foci, etc, the detection and filtering of outliers over the assay plate, and finally providing a set of statistics of the parameters studied together with a set of plots that we believe significantly helps to the interpretation of the assay results.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/cbdd.13616 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fingerprinting Single and Clustered Cu Sites in Metalated MOF Catalysts via TD-DFT and In Situ Diffuse Reflectance UV-Vis Spectroscopy.
ACS Appl Mater Interfaces
September 2025
Leibniz-Institut für Katalyse e.V. (LIKAT), Albert-Einstein-Str. 29a, Rostock 18059, Germany.
Metal-organic frameworks (MOFs) are transformative platforms for heterogeneous catalysis, but distinguishing atomically dispersed metal sites from subnanometric clusters remains a major challenge. This often demands the integration of multiple characterization techniques, many of which either lack the resolving power to distinguish active sites from their surrounding environments (e.g.
View Article and Find Full Text PDFImpact of remote follow-up under an intelligent medical collaboration model on health promotion and clinical outcomes in patients with urinary calculi.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
May 2025
Department of Outpatient, Third Xiangya Hospital, Central South University, Changsha 410013.
Objectives: Urinary calculi are characterized by a high recurrence rate, and patients' adherence to self-management after discharge directly affects health outcomes. Traditional offline follow-up models often face problems such as poor compliance and uneven allocation of medical resources, making it difficult to meet individualized health management needs. Remote follow-up provides a novel solution to optimize long-term management, improve health literacy, and enhance clinical outcomes.
View Article and Find Full Text PDFMolecular engineering based on four-arm perylene diimide chromophores toward hypoxia-induced specific photothermal therapy.
J Mater Chem B
September 2025
Key Laboratory of Medical Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Perylene diimide (PDI) radical anions have attracted increasing attention as hypoxia-responsive photothermal agents due to their strong near-infrared (NIR) absorption and efficient photothermal conversion. However, their biomedical application is often limited by aggregation-induced quenching and poor structural tunability. In this work, we report a rationally engineered four-arm PDI derivative (PDI-4Alky·4Cl) bearing terminal alkyne groups, which not only suppresses π-π stacking steric and electrostatic repulsion, but also serves as a versatile molecular scaffold for further functionalization.
View Article and Find Full Text PDFA novel label-free NIR aptasensor based on triphenylmethane dyes for rapid detection of salicylic acid.
Anal Methods
September 2025
Henan Linker Technology Key Laboratory, College of Advanced Interdisciplinary Science and Technology (CAIST), Henan University of Technology, Zhengzhou 450001, China.
Salicylic acid (SA) is a critical phytohormone involved in plant growth, development, and defense responses, making its precise quantification essential for both agricultural management and environmental monitoring. Here, we report a novel label-free near-infrared aptasensor (NIRApt) for the rapid and sensitive detection of SA, utilizing a rationally selected triphenylmethane (TPM) dye. Through systematic screening, ethyl violet (EV) was identified as the optimal fluorophore, showing pronounced fluorescence enhancement upon binding to a SA-specific aptamer.
View Article and Find Full Text PDFAdhesion-based cell sorting platform using on-chip centrifugation.
Lab Chip
September 2025
Department of Mechanical Systems Engineering, Graduate School of Systems Design, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
Cell sorting is an important fundamental process for the selection and purification of target cell types for cell analysis in the life sciences and medical fields. In particular, demand is increasing for high-throughput cell sorting technology for the analysis of rare cells. Toward this end, we developed a centrifugal force-based cell sorting technique that relies on the adhesion force of cells as a marker.
View Article and Find Full Text PDF