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Xylem is an essential conductive tissue in vascular plants, and secondary cell wall polymers found in xylem vessel elements, such as cellulose, hemicellulose, and lignin, are promising sustainable bioresources. Thus, understanding the molecular mechanisms underlying xylem vessel element differentiation is an important step towards increasing woody biomass and crop yields. Establishing in vitro induction systems, in which vessel element differentiation is induced by phytohormonal stimuli or by overexpression of specific transcription factors, has been vital to this research. In this review, we present an overview of these in vitro induction systems, and describe two recently developed in vitro induction systems, VISUAL (Vascular cell Induction culture System Using Arabidopsis Leaves) and the KDB system. Furthermore, we discuss the potentials and limitations of each of these new in vitro induction systems for advancing our understanding of the molecular mechanisms driving xylem vessel element differentiation.
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http://dx.doi.org/10.5511/plantbiotechnology.18.1119b | DOI Listing |
Plant Cell Environ
September 2025
Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, Taiwan.
Single-cell RNA sequencing (scRNA-seq) has emerged as a transformative tool for decoding plant development, particularly in elucidating xylem differentiation. By capturing transcriptomic changes at single-cell resolution, scRNA-seq enables reconstruction of developmental trajectories across diverse plant tissues. In this review, we summarize recent advances in the application of scRNA-seq to study both primary and secondary xylem development in monocots and eudicots.
View Article and Find Full Text PDFPlant Biol (Stuttg)
September 2025
Department of Agriculture Food and Environment, University of Catania, Catania, Italy.
In plants, xylem is directly involved in conveyance of water and dissolved minerals, mechanical support of the plant, and tolerance to drought stress. Moreover, for several fruit crops affected by vascular diseases, an association between the morphology of xylem vessels and susceptibility was described. In fact, compartmentalization represents a key determinant mechanism of plant resistance to vascular infections.
View Article and Find Full Text PDFGenome Biol
August 2025
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, USA.
Background: Single-cell genomics is revolutionizing plant developmental biology, enabling the transcriptome profiling of individual cells and their lineage relationships. However, plant cell walls polymers hamper the dissociation and analysis of intact cells. This rigid structure can conceal cell types embedded in complex, lignified, multi-cell layered tissues such as those undergoing secondary growth.
View Article and Find Full Text PDFSci Rep
August 2025
Department of Food Science and Technology, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab Emirates.
Understanding the physicochemical and structural properties of the dietary fiber present in date fruits is useful for its onward processing for food and non-food applications. This research fractionated dietary fiber from four date fruit cultivars into soluble and insoluble fractions and studied their structural features and physicochemical and thermal properties. Total dietary fibers content ranged 3.
View Article and Find Full Text PDFNew Phytol
August 2025
Department of Forest Science, Faculty of Agriculture and Forestry, University of Helsinki, Helsinki, 00790, Finland.
The Granier-type thermal-dissipation method (TDM) is the most widely used sap-flow technique. However, its original calibration coefficients often underestimate high flow rates, limiting their generality. We derived TDM coefficients (scaling factors and exponents) for 31 species, including 18 diffuse-porous, two ring-porous, six palms, and five lianas, representing a broad range of wood properties.
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