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The susceptibility of rice lines, T1C-19, T2A-1, and MH63 to SRBSDV infection are similar and the contents of cry protein in T2A-1 and T1C-19 do not change significantly. The survival rates of BPH nymphs feeding on SRBSDV-infected T1C-19, Bt T2A-1, or MH63 rice plants were not significantly different. The developmental stages of female BPH fed on T1C-19 plants infected with SRBSDV were significantly shorter than those fed on uninfected rice, while the males showed no significant difference. The duration of BPH feeding on SRBSDV-infected T2A-1 and MH63 also showed no significant difference in comparison with the respective control groups. Longevities of BPH adults feeding on SRBSDV-infected T1C-19, T2A-1 or MH63 were also not significant. However, the longevity of male adult BPH feeding on un-infected MH63 was significantly reduced in comparison with that of adult males feeding on un-infected T1C-19 and T2A-1 rice. In addition, the different rice lines and the rice plants infected and uninfected with SRBSDV did not significantly affect the sex ratio, female body weight, longevity, fecundity, or egg hatchability of BPH. In general, transgenic Bt rice infected with SRBSDV had little effect on the ecological adaptability of BPH.
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http://dx.doi.org/10.1038/s41598-017-02218-w | DOI Listing |
Int J Biol Macromol
May 2024
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Hubei Hongshan Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China; Pest Management and Biocontrol Research Unit, US Arid Land Agricultural Research
Bacillus thuringiensis (Bt) toxins have provided exceptional control of agricultural insect pests, however, over reliance on the proteins would potentially contribute to the development of field tolerance. Developing new sustainable insect pest control methods that target the mechanisms underlying Bt tolerance can potentially support the Bt control paradigm while also providing insights into basic insect physiology. The MAPK p38 pathway is strongly associated with Bt tolerance in Chilo suppressalis, a major pest of rice.
View Article and Find Full Text PDFJ Agric Food Chem
February 2021
Development Center of Science and Technology, Ministry of Agriculture of People's Republic of China, Beijing 100025, China.
Multiplex and high-throughput assays are becoming the main trends in the development of new nucleic acid detection and quantification methods, such as those for genetically modified organism (GMO) analysis. Here, we report a novel universal LNA probe-mediated droplet digital polymerase chain reaction (PCR) method (ULNA-ddPCR) for multiple DNA target quantification in GMOs. In ULNA-ddPCR, only one universal LNA probe is used for multiple DNA targets instead of using one to one TaqMan probe.
View Article and Find Full Text PDFJ Sci Food Agric
March 2021
Biotechnology Research Institute, Chinese Agricultural and Academic Sciences, Beijing, PR China.
Background: An isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis was employed to study the seeds of two genetically modified (GM) rice lines, T2A-1 and T1C-19, and their nontransgenic isogenic variety, MH63, to investigate the unintended effects of genetic modification.
Results: A total of 3398 proteins were quantitatively identified. Seventy-seven differentially abundant proteins (DAPs) were identified in the T2A-1/MH63 comparison, and 70 and 7 of these DAPs were upregulated and downregulated, respectively.
Pestic Biochem Physiol
January 2020
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Wuhan 430070, Hubei, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address:
Bacillus thuringiensis (Bt) insecticide is currently the most widely used bioinsecticide. Bt expressing cry genes are some of the most successful foreign-genome-inserting genes used in transgenic insect-resistant crop development. Cry toxins are resistant to lepidopteran pests, such as Chilo suppressalis, a major insect pest of rice worldwide.
View Article and Find Full Text PDFInsecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis (Bt) are toxic to a diverse range of insects. Transgenic rice expressing Cry1A, Cry2A and Cry1C toxins have been developed that are lethal to Chilo suppressalis, a devastating insect pest of rice in China. Identifying the mechanisms underlying the interactions of Cry toxins with susceptible hosts will improve both our understanding of Cry protein toxicology and long-term efficacy of Bt crops.
View Article and Find Full Text PDF