Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Staphylococcus aureus is a Gram-positive pathogen that can cause chronic skin inflammation, pneumonia, and septic shock. The immunomodulatory functions of wall teichoic acid (WTA), a glycopolymer abundantly expressed on the Gram-positive bacterial cell wall, are poorly understood. Here, we investigated the role of WTA in the phenotypic and functional activation of human monocyte-derived dendritic cells (DCs) treated with ethanol-killed S. aureus. WTA-deficient S. aureus mutant (ΔtagO) exhibited attenuated binding and internalization to DCs compared to the wild-type. ΔtagO induced lower expression of maturation markers on and cytokines in DCs than the wild-type S. aureus. Furthermore, autologous human peripheral blood mononuclear cells cocultured with ΔtagO-treated DCs exhibited a marked reduction in T cell proliferative activity, the expression of activation markers, and the production of cytokines compared to the wild-type S. aureus-stimulated DCs. Collectively, these results suggest that WTA is an important cell wall component of S. aureus for the induction of DC maturation and activation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.molimm.2016.12.008 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In Vivo Emergence of Podovirus Resistance via Mutation During Phage-Antibiotic Treatment of Experimental MSSA Endocarditis.
Viruses
July 2025
Laboratory of Bacteriophages and Phage Therapy, Center for Research and Innovation in Clinical Pharmaceutical Sciences (CRISP), Lausanne University Hospital (CHUV), CH-1011 Lausanne, Switzerland.
Phage therapy shows promise as an adjunct to antibiotics for treating Staphylococcus aureus infections. We previously reported a combined flucloxacillin/two-phage cocktail treatment selected for resistance to podovirus phage 66 in a rodent model of methicillin-susceptible (MSSA) endocarditis. Here we show that resistant clones harbor mutations in , which encodes a glycosyltransferase essential for β-GlcNAcylation of wall teichoic acid (WTA).
View Article and Find Full Text PDFAntibody dependent complement activation is critical for boosting opsonophagocytosis of Staphylococcus epidermidis in an extremely preterm human whole blood model.
Sci Rep
August 2025
Department of Paediatric Infectious Diseases and Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
Staphylococcus epidermidis is a major cause of late onset sepsis in extremely preterm neonates. Antibody therapies are considered as an interesting strategy to prevent sepsis. However, previous clinical trials with intravenous immunoglobulin (IVIG) and a monoclonal antibody (mAb) targeting staphylococcal lipoteichoic acid (LTA) (Pagibaximab) failed to show significant protection from invasive infections in extremely preterm neonates.
View Article and Find Full Text PDFIdentification of novel Listeria monocytogenes serotype 4h with specific antisera against wall teichoic acid.
J Microbiol Methods
September 2025
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Yangzhou University, Yangzhou,
Listeria monocytogenes (Lm) strains are serotyped based on their somatic (O) and flagellar (H) antigens. The newly designated serotype (ST) 4h exhibits a greater ability to colonize organs than well-characterized hypervirulent strains. Therefore, simple and rapid identification methods for Lm ST 4h are urgently needed for effective monitoring and prevention.
View Article and Find Full Text PDFDirect interaction between ZJ316-derived lipoteichoic acid and TLR2 mediates anti-inflammatory and barrier-protective effects in intestinal cells.
Food Funct
August 2025
Key Laboratory for Food Microbial Technology of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, People's Republic of China.
Lipoteichoic acid (LTA), a key bioactive substance of the Gram-positive bacterial cell wall, has garnered attention for its immunomodulatory properties. Herein, we investigated the underlying molecular mechanism by which LTA derived from ZJ316 exerts anti-inflammatory effects through interaction with Toll-like receptor 2 (TLR2). Molecular docking, dynamics simulations, and surface plasmon resonance (SPR) indicated a strong and specific binding affinity ( = 1.
View Article and Find Full Text PDFMechanistic studies on the effect of berberine on methicillin-resistant Staphylococcus aureus drug resistance through modulation of wall teichoteic acid.
Sci Rep
July 2025
Department of Clinical Laboratory, Shanghai Eighth People's Hospital, Shanghai, China.
The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a major public health concern, particularly in hospital- and community-acquired infections, underscores the urgent need for novel antibiotic therapies. In response to this challenge, there has been renewed interest in exploring natural products derived from traditional plant sources as potential alternatives for combating multi-drug resistance. This study reveals the important mechanism by which the natural compound berberine blocks the WTA biosynthesis pathway by targeting and inhibiting the key enzymes TarO, TarS, and TarM for the synthesis of muramic acid (WTA) in MRSA.
View Article and Find Full Text PDF