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In this work, we developed a novel light-up nanocluster beacon (NCB) based on shuttling dark silver nanoclusters (NCs) to a bright scaffold through hybridization. The fluorescence enhancement was as high as 70-fold when the two templates were on the opposite sides of the duplexes, enabling sensitive and selective detection of DNA.
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http://dx.doi.org/10.1039/c5cc09138a | DOI Listing |
Anal Chim Acta
August 2025
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China. Electronic address:
Background: Antibiotic resistance genes (ARGs) are emerging environmental contaminants that pose serious threats to human health and ecological security due to their rapid migration and transformation in the environment. Thus, developing a sensitive and rapid method to detect ARGs is significant.
Results: In this study, the neighborhood-dependent fluorescent sensor was established with DNA templated silver nanoclusters (AgNCs) and multi-branched linear structure to detect the typical macrolide resistance gene ermB.
Anal Chem
April 2025
Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
It might be intriguing to explore the tailored ability of a bihairpin-structured molecular beacon (MB) for improving reaction kinetics and assay sensitivity for a label-free ratiometric fluorescence assay of a specific trigger (*), in which red and green silver nanoclusters (SNC and SNC) were utilized as two reverse emitting reporters. Unlike traditional dually labeled MB, herein the proposed MB was designed with two hairpin subunits for specific recognition and signaling readout. Four functional modules were included: the complement of * in the identifiable hairpin, the template sequence of SNC and SNC in the signaling hairpin, two variable consensus loops capable of complementarily hybridizing to form one paired stem, and two stems able to merge into one new loop.
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May 2025
College of Chemistry and Molecular Engineering. Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:
In this work, a new dual-signal fluorescence strategy based on nano-gold molecular beacon (MB) and in-situ generated silver nano-clusters (NCs) coupled with multiple amplification technique was developed for sensitive detection of miRNA (let-7b). miRNA can recognize both hairpin probe (HP) and auxiliary DNA, inducing dual-cycle amplification-process to release plenty of DNA S2. As the report probe carboxyfluorescein (FAM) was modified on Au nanoparticles (AuNPs), the fluorescent signal was quenched due to the fluorescence resonance energy transfer (FRET).
View Article and Find Full Text PDFTalanta
June 2024
State Key Laboratory of Food Science and Resources, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Lihu Road 1800, Wux
In recent years, the conventional preparation of silver nanoclusters (AgNCs) has attracted much attention due to their ultra-small size, tunable fluorescence, easy-to-engineer, as well as biocompatible material. Moreover, its great affinity towards cytosine bases on single-stranded DNA has led to the construction of biosensors, especially aptamers, for a broad variety of applications in food safety and environmental protection. In past years, numerous researchers paid attention to the construction of AgNCs aptasensor.
View Article and Find Full Text PDFAnal Chem
February 2024
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
To address the limitations of typical hairpin-structural molecular beacons, exploring the ability of a quasi-molecular beacon (MB) to create label-free fluorescence biosensors is intriguing and remains a challenge. Herein, we propose the first example of modular MB with the feature of a stimulation-responsive conformation switch to develop an aggregated Ag nanocluster (AgNC) in a bifurcated DNA scaffold for fluorescently sensing a specific initiator (*). This MB was well designed to program four functional modules: *-recognizable element adopting metastable stem-loop bihairpin structure and two DNA splits (exposed CGT and locked CACT) of AgNC template that is separated by a tunable hairpin spacer for the customized combination of selective recognition and signaling readout.
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