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http://dx.doi.org/10.1051/medsci/201127133 | DOI Listing |
J Extracell Vesicles
September 2025
Department of Pharmacology and Therapeutics, School of Medicine, Universidad Autónoma de Madrid, Madrid, Spain.
Although the field of plant EVs (PEVs) is experiencing exponential growth, rigorous characterisation complying with MISEV guidelines has not been yet implemented due to the lack of bona fide reference markers. In this work, we have paved the way for the standardisation of PEV markers, providing the most profound proteomic data so far from apoplastic washing fluid-EVs, a sample enriched in genuine extracellular vesicles from plant tissue of two reference plant species: Arabidopsis thaliana (Arath-EVs) and Brassica oleracea (Braol-EVs). Besides, we analysed the protein content of the soluble fraction of the apoplast and calculated the enrichment of the potential markers studied in EVs.
View Article and Find Full Text PDFGenes (Basel)
August 2025
Department of Basic Sciences, University of Agriculture in Krakow, Redzina 1C, 30-248 Krakow, Poland.
Mesenchymal stem cells (MSCs) are a promising tool in regenerative medicine due to their ability to secrete paracrine factors that modulate tissue repair. Extracellular vesicles (EVs) released by MSCs contain bioactive molecules (e.g.
View Article and Find Full Text PDFInt J Mol Sci
August 2025
Regenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung 807, Taiwan.
Articular cartilage (AC) has a very limited capacity for self-healing once damaged. Chondrocytes maintain AC homeostasis and are key cells in AC tissue engineering (ACTE). However, chondrocytes lose their function due to oxidative stress.
View Article and Find Full Text PDFMedicine (Baltimore)
August 2025
Tianjin Eye Hospital, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin Eye Institute, Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
Our study aims to elucidate the impact of exosomes obtained from human umbilical cord mesenchymal stem cells (MSC-Exos) on the proliferation, migration, and oxidative stress response of human retinal vascular endothelial cells (HRECs) and the mechanism. MSC-Exos were isolated from human umbilical cord mesenchymal stem cells using a low-temperature ultrahigh-speed centrifugation method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, scratch tests, and cellular immunofluorescence assays were utilized to assess the influence of MSC-Exos on proliferation, migration, and oxidative stress in HRECs.
View Article and Find Full Text PDFPhytomedicine
October 2025
Department of Endocrinology and Metabolism, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:
Background: β-cell dedifferentiation and impaired maturation are crucial contributors to β-cell dysfunction in type 2 diabetes mellitus (T2DM), yet targeted therapies remain limited.
Objective: To investigate the protective effects of Ramulus Mori (Sangzhi) Alkaloids (SZ-A) on β-cell function and maturation in T2DM.
Methods: A T2DM mouse model was induced by high-fat diet and streptozotocin, followed by SZ-A treatment.