Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Bipolaris sorokiniana is an economically important phytopathogen of wheat and other cereal species. In this paper, a novel pathogen-induced ethylene-responsive factor (ERF) gene of wheat, TaPIEP1, was isolated and characterized. The transcript of TaPIEP1 was significantly and rapidly induced by treatments with B. sorokiniana, and with ethylene (ET), jasmonate (JA), and abscisic acid. Molecular and biochemical assays demonstrated that TaPIEP1 is a new ERF transcription activator belonging to B-3c subgroup of the ERF family. Transgenic wheat lines overexpressing TaPIEP1 were generated by biolistic bombardment and molecular screening. Compared with the host wheat Yangmai12, six stable transgenic wheat lines overexpressing TaPIEP1 that exhibited significantly increased resistance to B. sorokiniana were identified by molecular detection in the T(0)-T(4) generations and by disease resistance tests. The degree of the enhanced resistance was correlated with an accumulation of the transcript level of TaPIEP1. Furthermore, the transcript levels of certain defense-related genes in the ET/JA pathways were markedly increased in the transgenic wheat plants with enhanced resistance. These results reveal that TaPIEP1 overexpression in wheat could obviously improve resistance to B. sorokiniana via activation of some defense genes, and TaPIEP1 gene may be useful in improving crop resistance to the pathogen.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10142-009-0157-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
CRISPR RNP-Mediated Transgene-Free Genome Editing in Plants: Advances, Challenges and Future Directions for Tree Species.
Plant Cell Environ
September 2025
State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Bamboo Research Institute, Key Laboratory of National Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, School of Life Sciences, Nanjing Forestry
CRISPR ribonucleoprotein (RNP)-mediated genome editing offers a transgene-free platform for precise genetic modification in diverse herbaceous and tree species, including rice, wheat, apple, poplar, oil palm, rubber tree and grapevine. However, its application in woody plants faces distinct challenges, notably inefficient delivery and regeneration difficulties, particularly in species such as bamboo. While some of these issues also occur in herbaceous plants, they are often significantly more complex in woody species due to factors such as intricate cell wall architecture, widespread recalcitrant genotypes and inherent limitations of current delivery platforms.
View Article and Find Full Text PDFFenoxaprop-P-ethyl resistance in Alopecurus aequalis: Involvement of GSTF13 in addition to Ile-1781-Leu mutation.
Pestic Biochem Physiol
November 2025
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China. Electronic address:
Shortawn foxtail (Alopecurus aequalis Sobol.) is a challenging weed species to manage in wheat production systems globally. In prior research, we identified a field population of A.
View Article and Find Full Text PDFFine tuning wheat development for the winter to spring transition.
Plant Commun
September 2025
School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany. Electronic address:
The coordination of floral developmental stages with the environment is important for reproductive success and the optimization of crop yields. The timing of different developmental stages contributes to final yield potential with optimal adaptation enabling development to proceed without being impacted by seasonal weather events, including frosts or end of season drought. Here we characterise the role of FLOWERING LOCUS T 3 (FT3) in hexaploid bread wheat (Triticum aestivum) during the early stages of floral development.
View Article and Find Full Text PDFModification of starch traits in commercial wheat through TaWaxy gene editing.
Carbohydr Polym
November 2025
State Key Laboratory of Crop Gene Resources and Breeding, National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address:
Amylose content (AC) is a key determinant of wheat quality, and the TaWaxy gene determined amylose synthesis with a dose-dependent effect on AC. In this study, the TaWOX5 gene, which significantly enhances wheat transformation efficiency, was combined with CRISPR/SpCas9 system to generate TaWaxy mutants in a commercial winter wheat Jimai 22. Seven transgene-free mutant types were produced, compared to only three transgene-free mutants in the spring wheat variety Ningchun 4.
View Article and Find Full Text PDFReduced stomatal density in wheat overexpressing EPIDERMAL PATTERNING FACTOR1 differentially affects red and blue light responses.
Plant Physiol
September 2025
School of Life Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom.
Stomatal pores govern the tradeoff between CO₂ assimilation and water loss, and optimizing their performance is critical for crop resilience, particularly under dynamic field environments. Here, we show that overexpression of Triticum aestivum EPIDERMAL PATTERNING FACTOR1 (TaEPF1) in bread wheat (Triticum aestivum) reduces leaf stomatal density in a leaf surface-specific manner, with a greater decline on the abaxial surface than on the adaxial surface. TaEPF1 overexpressors exhibited substantially lower stomatal conductance than wild-type (WT) control plants, which resulted in diffusional constraints limiting photosynthesis when measured under monochromatic red light.
View Article and Find Full Text PDF