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Article Abstract
Purpose: To observe the effects of electric fields (EFs) on the migration of human retinal pigment epithelial (RPE) cells and to explore possible related mechanisms.
Methods: Cultured human RPE cells were exposed to EFs, and images of the cells were obtained at regular intervals to evaluate the cell migration and viability. Distribution of F-actin and beta1 integrin was measured by immunohistochemistry. Expression of beta1 integrin was determined by PCR and Western blotting.
Results: Exposure to EFs resulted in a cathodal-directed migration of RPE cells and a cathodal accumulation of F-actin and beta1 integrin in the cells. EF stimulation increased expression of beta1 integrin both in mRNA and protein levels. These effects could be inhibited by cytochalasin B.
Conclusion: EFs induce directed migration of RPE cells, and this effect is, to some extent, regulated by the interaction between cytoskeleton and integrins.
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| http://dx.doi.org/10.1080/02713680902879033 | DOI Listing |
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