Visual outcome of 25 Gauge vitrectomy for acute post operative infectious endophthalmitis.

Background: The correlation between the change in foveal thickness measured using optical coherence tomography (OCT) following surgery for infectious endophthalmitis and preoperative and postoperative visual acuity is uncertain, and there are few pertinent studies on this topic.

Objective: We explored the variations in macular thickness using OCT after emergency vitrectomy for post-cataract infectious endophthalmitis and the relationship between macular thickness with changes in visual function.

Methods: We included 10 cases of post-cataract infectious endophthalmitis.

microRNA-218 Inhibits Oxygen-induced Retinal Neovascularization via Reducing the Expression of Roundabout 1.

Background: The mechanisms of pathological retinal neovascularization (RNV) remain unknown. Several microRNAs were reported to be involved in the process of RNV. Oxygen-induced retinopathy (OIR) is a useful model to investigate RNV.

Change of Retinal Nerve Layer Thickness in Non-Arteritic Anterior Ischemic Optic Neuropathy Revealed by Fourier Domain Optical Coherence Tomography.

Purpose: To examine the changes of non-arteritic anterior ischemic optic neuropathy (NAION) by serial morphometry using Fourier domain optical coherence tomography (FD-OCT).

Materials And Methods: Retrospective study in patients with newly diagnosed NAION (n=33, all unilateral) and controls (n=75 unilateral NAION patients with full contralateral eye vision) who underwent FD-OCT of the optic disk, optic nerve head (ONH), and macula within 1 week of onset and again 1, 3, 6, and 12 months later. The patients showed no improvement in vision during follow-up.

Integrin β1 subunit signaling is involved in the directed migration of human retinal pigment epithelial cells following electric field stimulation.

Aims: Direct current electric fields (EFs) can induce directed cell migration in a wide variety of cells, and this has been proven to be of importance in wound healing. Here we observed the effects of EFs on cultured human retinal pigment epithelial (RPE) cells and explored the possible involvement of integrin β1 subunit signaling in the process.

Methods: Cultured human RPE cells were exposed to an EF at 5 V/cm for 3 h.

New modified iris suture technique for pupillary dilation in aphakic eyes during vitreoretinal surgery.

To describe a modified simple iris suture for pupillary dilation technique during vitrectomy in cases with a miotic pupil. Four translimbal incisions were created with a sharp straight blade at 1:30, 10:30, 4:30, and 7:30 o'clock, respectively. The straight needle of 10-0 polypropylene suture and a Sinskey IOL hook was used to displace the pupillary margin toward the limbus.

Electric fields contribute to directed migration of human retinal pigment epithelial cells via interaction between F-actin and beta1 integrin.

Purpose: To observe the effects of electric fields (EFs) on the migration of human retinal pigment epithelial (RPE) cells and to explore possible related mechanisms.

Methods: Cultured human RPE cells were exposed to EFs, and images of the cells were obtained at regular intervals to evaluate the cell migration and viability. Distribution of F-actin and beta1 integrin was measured by immunohistochemistry.

Modulation of migration and Ca2+ signaling in retinal pigment epithelium cells by recombinant human CTGF.

Purpose: The migration of retinal pigment epithelium (RPE) cells is an initial step in the development of proliferative vitreoretinopathy (PVR). We investigated the expression of connective tissue growth factor (CTGF) in an in vitro model of wound healing and effects of recombinant human CTGF (rhCTGF) on modulating migration and Ca(2+) signaling in RPE cells.

Methods: Cultured human RPE monolayers were used to establish a wound-healing model.

Mechanical force enhances MMP-2 activation via p38 signaling pathway in human retinal pigment epithelial cells.

Background: Rhegmatogenous retinal detachment and proliferative vitreoretinopathy (PVR) are eye diseases that are characterized by mechanical stress involving stretching of the retinal pigment epithelial (RPE) cells by the vitreous or the hyperplastic membranes. Here, we assessed whether mechanical force could change the expression of matrix metalloproteinases (MMPs) in RPE cells via the mitogen-activated protein kinase (MAPK) pathway.

Methods: Collagen-coated magnetite beads and magnetic fields were used to apply tensile forces to cultured RPE cells at focal adhesions.

[Effects of magnetic field on MAPK signaling pathways of human retinal pigment epithelial cells bound with beads in vitro].

Objective: To observe the changes of mitogen activated protein kinase (MAPK) signaling pathways of human retinal pigment epithelial cells( RPEs) bound with beads under stretch caused by the force from magnetic field in vitro.

Methods: Ferric oxide microparticles, coAted with collagen, were added to dishes containing substrate-attached RPEs. After incubation, the cell layer bound with beads was laid in a magnetic field, the cells were stretched by the magnetic force.

[Experimental study of acellular xenogenic dermal matrix and allogeneic sclera as wrapping materials for hydroxy apatite implantation].

Objective: To observe the experimental results and histopathological changes of acellular xenogenic dermal matrix (X-ADM) and allogeneic sclera used as wrapping materials of hydroxy apatite (HA) ocular implants in New Zealand white rabbits.

Methods: Twenty-four rabbits received unilateral eye enucleating and the sockets were implanted with HA spherical implants wrapped with either acellular xenogenic dermal matrix or allogeneic sclera at random. The rabbits were examined for inflammation and implant exposure and sacrificed at 1, 2, 4, 6, 8 and 12 weeks after implantation.

[Cytoskeleton changes of cultured human retinal pigment epithelial cells in a mechanical stress model].

Objective: To observe changes of the cytoskeleton of cultured human retinal pigment epithelium (hRPE) cells in a mechanical stress model in vitro.

Methods: Ferric oxide beads, coated with collagen, were added to dishes containing substrate-attached hRPE cells. After incubation, the cells were washed to remove unbound beads.

[Effects of mechanical stress on expressions of monocyte chemoattractant protein-1 and interleukin-8 of cultured human retinal pigment epithelial cells].

Objective: To investigate the expressions of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) of cultured human retinal pigment epithelial (hRPE) cells under mechanical stress in vitro, so as to mimic and understand the role of these 2 inflammatory cytokines in the early stage of development of primary retinal detachment.

Methods: Ferric oxide beads coated with collagen were added to the culture plate containing wall-attaching hRPE cells and then the cells were incubated and washed to remove the unbound beads. A magnet was put to the culture plate to provide vertical magnetic force.

Transscleral suture technique for fixation of a dislocated posterior chamber intraocular lens.

We describe a double-loop-knot technique for repositioning a displaced posterior chamber intraocular lens (IOL) that facilitates placement of scleral fixation sutures around the haptic for IOL stabilization. The technique minimizes the intraocular manipulations necessary to create a suture loop around the haptic of a dislocated IOL as well as scleral incisions required for IOL exchange.