Deficiency of nNOS in adult-born dentate granule cells causes epilepsy.

Objective: This study aimed to elucidate the molecular role of neuronal nitric oxide synthase (nNOS, encoded by Nos1) in adult-born dentate granule cells (DGCs) during temporal lobe epilepsy (TLE).

Methods: We used GFP-expressing retrovirus (RV) to analyze morphological changes in DGCs. Nos1 knockout (Nos1) mice were generated to assess whether nNOS deficiency would induce mossy fiber sprouting (MFS), affect neurogenesis, and observe the morphological changes of DGCs.

[Moxibustion for central obesity with phlegm-dampness constitution: a randomized controlled trial].

Objective: To observe the efficacy and safety of moxibustion in treating patients with central obesity of phlegm-dampness constitution.

Methods: A total of 66 patients with central obesity of phlegm-dampness constitution were randomly assigned to a moxibustion group (=33, 3 cases dropped out) and a sham moxibustion group (=33, 4 cases dropped out). The moxibustion group received mild moxibustion combined with lifestyle intervention; the moxibustion was applied at Shenque (CV8) and bilateral Zusanli (ST36), 30 min per session, maintaining a local skin temperature of (43±1) ℃.

[Effect of electroacupuncture on the permeability of blood-brain-barrier mediated by Wnt7a/β-catenin/GSK-3β pathway in mice with Parkinson's disease].

Objectives: To observe the effect of electroacupuncture (EA) on the permeability of blood-brain-barrier (BBB) and the regulation of the wingless-type mouse mammary tumor virus integration site 7a (Wnt7a)/β-catenin/glycogen synthase kinase-3β (GSK-3β) pathway in Parkinson's disease (PD) mice.

Methods: Thirty C57 BL/6 mice were randomly divided into normal, model and EA groups, with 10 mice in each group. The PD model was established by intraperitoneal injection of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP, 30 mg/kg), once daily for 7 days.

[Efficacy of " acupoint compatibility" moxibustion for abdominal obesity and its effect on lipid accumulation].

Objective: To observe the efficacy of " acupoint compatibility" moxibustion for abdominal obesity and its effect on blood lipid, lipid accumulation product (LAP) and cardiometabolic index (CMI).

Methods: A total of 150 patients with abdominal obesity were randomly divided into an observation group (75 cases, 5 cases dropped out) and a control group (75 cases, 6 cases dropped out). The control group received lifestyle guidance.

Virtual Screening of Kelch-like ECH-Associated Protein 1-Nuclear Factor Erythroid 2-Related Factor 2 (Keap1-Nrf2) Inhibitors and In Vitro Validation.

The transcription factor erythroid 2-related factor 2 (Nrf2) is a central regulator of cellular defense mechanisms against oxidative stress and inflammation. Keap1 (Kelch-like ECH-associated protein 1) regulates Nrf2 activity by ubiquitination-mediated cytoplasmic retention, thereby suppressing its nuclear translocation and subsequent transcriptional activation of genes encoding phase II detoxifying enzymes. Using a structure-based virtual screening approach, we screened ~16,000 natural compounds to identify Keap1-Nrf2 PPI inhibitors.

[Electroacupuncture intervention promotes browning of white adipose tissue by regulating LKB1/AMPK pathway in obese mice].

Objectives: To observe the effect of electroacupuncture (EA) on hypothalamic liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling pathway, sympathetic nerve activity and browning of white adipose tissue (WAT) in obese mice, so as to explore the possible central mechanisms of EA in improving obesity.

Methods: C57BL/6J male mice were divided into normal group (=8) and high fat diet group (=30) randomly, and fed by normal diet and high fat diet for 8 weeks, respectively. In the high fat diet group, mice with body weight over 20% heavier than that of the normal group were considered as obese mice.

Progress in Oplopanax elatus research: Resources, propagation, traditional uses, phytochemistry, pharmacology, pharmacokinetics, quality control, and toxicity.

Ethnopharmacological Relevance: Oplopanax elatus Nakai is a valuable medicinal plant of the Araliaceae family. In China, South Korea, and Russia, O. elatus has a long history of ethnomedical use, particularly for treating neurasthenia, schizophrenia, cardiovascular disease, and other conditions.

Polyethylene glycol loxenatide modulates lipid metabolism and insulin resistance through lncRNA steroid receptor RNA activator/cellular nucleic acid binding protein/Rho-associated coiled-coil kinase 2 axis in type 2 diabetes mellitus.

Background: Polyethylene glycol loxenatide (PEG-Loxe) is applied in treating type 2 diabetes mellitus. Nevertheless, the effect and mechanism of PEG-Loxe on lipid metabolism disorder and insulin resistance in type 2 diabetes mellitus are not fully understood.

Methods: Type 2 diabetes mellitus rats developed by high-fat diet/streptozotocin injection were treated with PEG-Loxe (0.

Medicinal potential of adventitious root cultures of Hypericum perforatum: study on antioxidant stress and anti-melanogenic properties.

Background: Adventitious root (AR) culture is an effective method for the production of Hypericum perforatum raw materials. However, the ARs are seldom utilized in practical applications. Therefore, this study investigated the effects of H.

Role of microRNAs in diabetic foot ulcers: Mechanisms and possible interventions.

Diabetic foot ulcer (DFU) is a common and serious complication among diabetic patients, and its incidence and difficulty in treatment have placed large burdens on patient health and quality of life. Diabetic foot tissue typically exhibits chronic wounds, ulcers, or necrosis that are difficult to heal, are prone to infection, and, in severe cases, may even lead to amputation. Recent studies have shown that microRNAs (miRNAs) play key roles in the development and healing of DFUs.

Effects of moxibustion on intestinal barrier function and TLR4/NF-κB p65 signaling pathway in obese rats.

Objectives: To observe the effects of moxibustion on intestinal barrier function and Toll-like receptor 4 (TLR4)/nuclear factor-κB p65 (NF-κB p65) signaling pathway in obese rats and explore the mechanism of moxibustion in the intervention of obesity.

Methods: Fifty-five Wistar rats of SPF grade were randomly divided into a normal group (10 rats) and a modeling group (45 rats). In the modeling group, the obesity model was established by feeding high-fat diet.

Efficacy of acupuncture therapy on cancer-related insomnia: a systematic review and network meta-analysis.

Objectives: Cancer-related insomnia (CRI) takes a toll on many cancer survivors, causing distressing symptoms and deteriorating the quality of life. Acupuncture therapy has been used for CRI already. However, it is still uncertain which acupuncture regime is best for CRI.

Comparative analysis of medicinal plants Scutellaria baicalensis and common adulterants based on chloroplast genome sequencing.

Background: Scutellaria baicalensis Georgi has been extensively used as a medicinal herb in China for over 2000 years. They may be intentionally or inadvertently substituted or blended with comparable species in the local market, threatening clinical medication safety. Molecular markers are effective tools to prevent misidentification and eliminate doping and falsification among Scutellaria plants.

Traditional uses, phytochemistry, pharmacology, and safety concerns of hawthorn (Crataegus genus): A comprehensive review.

Ethnopharmacological Relevance: The genus Crataegus (hawthorn), a member of the Rosaceae family, encompasses several species with broad geographical distribution across the Northern Hemisphere, including Asia, Europe, and the Americas. Hawthorn is recognized as an edible medicinal plant with applications related to strengthening the digestive system, promoting blood circulation, and resolving blood stasis.

Aim Of The Review: This study critically summarized the traditional uses, phytochemistry, and pharmacological properties to provide a theoretical basis for further studies on hawthorn and its applications in medicine and food.

Tracking interface position of a high-speed imploding composite liner based on magnetic diffusion difference.

A technique for tracking the interface position of non-metal-metal composite liners during high-speed implosion is proposed in this paper. Based on the magnetic diffusion difference between metal and non-metal, the interface position information is obtained by measuring magnetic fields in the cavity of the liner. An efficient magnetic flux estimation algorithm based on iterative magnetic diffusion simulation is also proposed to estimate the magnetic flux loss of the liner.

A nonlinear associations of metabolic score for insulin resistance index with incident diabetes: A retrospective Chinese cohort study.

Background: The Metabolic score of insulin resistance (METS-IR) has recently been accepted as a reliable alternative to insulin resistance (IR), which was demonstrated to be consistent with the hyperinsulinemic-euglycemic clamp. Few pieces of research have focused on the relationship between METS-IR and diabetes in Chinese. The purpose of this research was to explore the effect of METS-IR on new-onset diabetes in a large multicenter Chinese study.

Microbial community diversity and function analysis of Debeaux in rhizosphere soil of farmlands in Southwest China.

Understanding how microbial communities affect plant growth is crucial for sustainable productivity and ecological health. However, in contrast with the crop system, there is limited information on the microbial community associated with the medicinal plant. We observed that altitude was the most influential factor on the soil microbial community structures of Debeaux.

Comparative analysis of medicinal plant and its common adulterants based on chloroplast genome sequencing.

(Hemsley) H. Hara is the source of Donglingcao under the monograph in Chinese Pharmacopoeia. In the local marketplace, this medicine can be accidentally contaminated, deliberately substituted, or mixed with other related species.

Exploring the Relationship between Allergic Rhinitis and Constitution Based on the "Traditional Chinese Medicine Constitution Theory".

Allergic rhinitis (AR) is a prevalent allergic disease affecting individuals of all ages, especially children and adolescents. Patients with AR develop a specific immunoglobulin E antibody response to allergens, including pollen, mold, dust mites, and animal dander. The main symptoms of AR patients include nasal congestion, rhinorrhea, sneezing, and nasal itching.

Discovery of Cyclic Diarylheptanoids as Inhibitors against Influenza A Virus from the Roots of .

Four new cyclic diarylheptanoids, casuarinols A-C (-) and casuarinolide A (), together with six known ones (-), were isolated from the roots of . Structures were elucidated by extensive spectroscopic analysis, theoretical conformational, and electronic circular dichroism analyses. Casuarinol C () is a novel cyclic diarylheptanoid-aldehyde adduct.

Efficacy and Safety of Moxibustion for Menopausal Obesity: A Multicentre, Randomized, Controlled Trial Protocol.

. In the past, moxibustion has been widely used to treat endocrine system disorders, but evidence of its effectiveness is scarce at this point. The aim of this multicenter, randomized, controlled trial is to evaluate the efficacy and safety of treating menopausal obesity with moxibustion.

Development of a transient complex impedance measurement device used in quasi-isentropic compression experiments.

A complex impedance measurement device with a short response time and high noise immunity is presented in this paper. The device based on a radio-frequency reflectometer was specially developed for electro-physical property investigations of materials in quasi-isentropic compression experiments. The maximum operating frequency of the device is up to 600 MHz for reducing intense low-frequency noises.