Dynamic Macropore Formation via Dual-Degrading Injectable Microgels Enhances Host-Mediated Angiogenesis and Regeneration.

Hydrogels are widely employed in tissue engineering for their biomimetic microenvironments. However, the dense crosslink network of hydrogels with matching mechanical properties of soft tissues often restricts cell infiltration and tissue integration. While granular hydrogels enhance host integration through the formation of porous channels between particles, they self-anneal in vivo, thereby limiting porosity and interconnectivity.

Central nervous system infection caused by Mycoplasma hominis in an adult: a case report and literature review.

Objective: To investigate the diagnostic and therapeutic approaches for M. hominis induced CNS infections in non-neonatal patients, raise clinical awareness, and provide insights for managing such cases.

Methods: A retrospective analysis was performed on the clinical presentation, laboratory findings, treatment, and prognosis of an adult meningitis case caused by M.

Machine learning-assisted multi-dimensional transcriptomic analysis of cytoskeleton-related molecules and their relationship with prognosis in hepatocellular carcinoma.

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide, with a poor prognosis due to its aggressive nature and limited treatment options. Cytoskeletal dynamics play a critical role in tumor progression, but the prognostic and therapeutic potential of cytoskeleton-related genes in HCC remains underexplored. In this study, transcriptomic data from the TCGA-LIHC dataset were used to identify differentially expressed cytoskeleton-related genes associated with overall survival (OS).

An mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite for cancer immunotherapy.

Hydrogel materials have emerged as versatile platforms for various biomedical applications. Notably, the engineered nanofiber-hydrogel composite (NHC) has proven effective in mimicking the soft tissue extracellular matrix, facilitating substantial recruitment of host immune cells and the formation of a local immunostimulatory microenvironment. Leveraging this feature, here we report an mRNA lipid nanoparticle (LNP)-incorporated NHC microgel matrix, termed LiNx, by incorporating LNPs loaded with mRNA encoding tumour antigens.

Differences in Intestinal Microbiota Between White and Common Cranes in the Yellow River Delta During Winter.

The avian intestinal microbiota is a vital interface for host/environment interactions, playing a pivotal role in nutrient metabolism, immune regulation, and ecological adaptation. In the Yellow River Delta region, common cranes and white cranes coexist in mixed flocks. During the winter, when food resources are scarce, studying their gut microbiota can effectively reveal the feeding patterns of these two crane species, thereby providing valuable data for crane conservation efforts.

Exosome-derived hsa_circ_0007132 promotes lenvatinib resistance by inhibiting the ubiquitin-mediated degradation of NONO.

Hepatocellular carcinoma (HCC) is a highly heterogeneous solid tumor, with its incidence showing a troubling upward trend over the past decade. Lenvatinib is one of the first-line medications for treating advanced HCC, however, the development of resistance significantly undermines its potential to improve patient prognosis. In recent years, exosomal circRNAs have been implicated in the resistance mechanisms of various cancers, yet their role in mediating lenvatinib resistance (LR) remains largely unexplored.

Exosome-derived lnc-FAM72D-3 promotes lenvatinib resistance by remodeling hepatocellular carcinoma cytoskeleton via MBNL1/FAK axis.

Lenvatinib resistance (LR) profoundly exacerbates the prognosis of patients afflicted with advanced hepatocellular carcinoma (HCC). As pivotal mediators of intercellular communication, exosomes have been implicated in the development of LR. Nonetheless, the precise contributions of exosome-derived long non-coding RNAs (lncRNAs) to this phenomenon remain inadequately elucidated.

Cardiovascular Fitness and Potential Risk of Work-Related Injuries in Manual Therapy Students: A Cross-Sectional Study.

Background Chiropractic and manual therapy professions are considered physically demanding and tend to be associated with high rates of work-related injuries. The reported injuries particularly affect the upper extremities and lower back. Despite extensive research on physical therapists, there are limited studies on chiropractors.

Post-treatment adverse events ranking in targeted immunotherapy for hepatocellular carcinoma: A network meta-analysis based on risk probability assessment.

Background: Despite the rapid evolution of targeted and immunotherapies for hepatocellular carcinoma (HCC), a systematic comparison of their adverse event profiles remains limited. This review addresses this critical gap by synthesizing data from 13 randomized controlled trials (RCTs) to prioritize treatment regimens on the basis of safety, thereby guiding clinical decision-making in an era of expanding therapeutic options.

Methods: Clinical studies focusing on targeted and immunotherapies in HCC patients were chosen from databases such as PubMed, Embase, Web of Science and the Cochrane Library, which spans from 2008 to 2023.

CircRNA-mTOR Promotes Hepatocellular Carcinoma Progression and Lenvatinib Resistance Through the PSIP1/c-Myc Axis.

Circular RNAs (circRNAs) are crucial regulators of targeted drug resistance in hepatocellular carcinoma (HCC). However, the specific mechanisms underlying resistance that significantly hampers the effectiveness of HCC treatments remain unclear. Here, it is found that circRNA-mTOR is highly expressed in HCC and strongly correlated with patient prognosis.

Complete mitochondrial genome of (passeriformes: cisticolidae).

The complete mitogenome of Orthotomus sutorius Pennant, 1769 this species was sequenced using an Illumina Novaseq platform. It is 16,982 bp in length and consists of 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes (12S rRNA and 16S rRNA) and a control region (CR). We reconstructed a phylogenetic tree based on the mitogenome sequences of 10 species from family Cisticolidae with its adjacent families, as well as one outgroup.

Comparative analysis of hepatectomy for HCC with PVTT: Insights from a 30-year single-center experience: Hepatectomy for HCC with PVTT.

Background And Aim: Portal vein tumor thrombosis (PVTT) is frequent in hepatocellular carcinoma (HCC). Although hepatectomy is the primary treatment for HCC, no consensus exists on its role in PVTT between Eastern and Western clinicians. This study aims to assess the efficacy of hepatectomy in HCC patients with PVTT by analyzing perioperative outcomes and prognosis.

The dietary patterns of water deer recently rediscovered in Northeast China exhibit remarkable similarities to those observed in other regions.

Climate change, human activities, and habitat fragmentation-or even loss-pose ongoing threats to the survival of wildlife. Understanding the dietary habits of endangered species is a critical component of their conservation. In this study, we investigated the diet of water deer (Hydropotes inermis) by integrating traditional fecal microhistological analysis with a deep learning algorithm.

ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity.

The ubiquitination of histone H2A/H2AX, catalyzed by RNF8/RNF168, is a crucial step in the repair of DNA double-strand breaks (DSBs), playing a significant role in transmitting and amplifying DNA damage response signals. However, the upstream regulatory mechanisms of RNF168 remain unclear. Here, we demonstrate that ZNF451 catalyzes the SUMOylation of RNF168, thereby regulating the ubiquitination of histone H2A/H2AX.

mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite generates a local immunostimulatory niche for cancer immunotherapy.

Hydrogel materials have emerged as versatile platforms for various biomedical applications. Notably, the engineered nanofiber-hydrogel composite (NHC) has proven effective in mimicking the soft tissue extracellular matrix, facilitating substantial recruitment of host immune cells and the formation of a local immunostimulatory microenvironment. Leveraging this feature, here we report an mRNA lipid nanoparticle (LNP)-incorporated NHC microgel matrix, termed LiNx, by incorporating LNPs loaded with mRNA encoding tumour antigens.

Innovative applications of advanced nanomaterials in cerebrovascular imaging.

Cerebrovascular imaging is essential for the diagnosis, treatment, and prognosis of cerebrovascular disease, including stroke, aneurysms, and vascular malformations. Conventional imaging techniques such as MRI, CT, DSA and ultrasound have their own strengths and limitations, particularly in terms of resolution, contrast and safety. Recent advances in nanotechnology offer new opportunities for improved cerebrovascular imaging.

Phospho-TRIM21 orchestrates RPA2 ubiquitination switch to promote homologous recombination and tumor radio/chemo-resistance.

RPA2, a key component of the RPA complex, is essential for single-stranded DNA (ssDNA) binding and DNA repair. However, the regulation of RPA2-ssDNA interaction and the recruitment of repair proteins following DNA damage remain incompletely understood. Our study uncovers a novel mechanism by which phosphorylated TRIM21 (Phospho-TRIM21) regulates RPA2 ubiquitination, thereby modulating homologous recombination and tumor radio/chemo-resistance.

CircPIK3C3 inhibits hepatocellular carcinoma progression and lenvatinib resistance by suppressing the Wnt/β-catenin pathway via the miR-452-5p/SOX15 axis.

Introduction: Resistance to lenvatinib limits the effectiveness of the targeted treatments for HCC. However, the exact mechanism behind this resistance remains elusive. Current research suggests that circular RNA (circRNA) is pivotal in mediating drug resistance during targeted treatments.

RRM1 promotes homologous recombination and radio/chemo-sensitivity via enhancing USP11 and E2F1-mediated RAD51AP1 transcription.

Ribonucleotide reductase M1 (RRM1), the catalytic subunit of ribonucleotide reductase, plays a pivotal role in converting ribonucleotides (NTP) into deoxyribonucleotides (dNTP), essential for DNA replication and repair. Elevated RRM1 expression is associated with various human cancers, correlating with poorer prognosis and reduced overall survival rates. Our previous study found that RRM1 will enter the nucleus to promote DNA damage repair.

Ex vivo expansion and hydrogel-mediated in vivo delivery of tissue-resident memory T cells for immunotherapy.

Tissue-resident memory T (T) cells preferentially reside in peripheral tissues, serving as key players in tumor immunity and immunotherapy. The lack of effective approaches for expanding T cells and delivering these cells in vivo hinders the exploration of T cell-mediated cancer immunotherapy. Here, we report a nanoparticle artificial antigen-presenting cell (nano-aAPC) ex vivo expansion approach and an in vivo delivery system for T cells.

Granular Nanofiber-Hydrogel Composite-Programmed Regenerative Inflammation and Adipose Tissue Formation.

The interplay between biomaterials and host immune responses critically determines outcomes in tissue restoration. Recent studies suggest that physicochemical properties of materials can dictate pro-regenerative versus pro-fibrotic responses and have begun to define the key immune cell types and signals governing these divergent effects. This emerging understanding enables the engineering of regenerative biomaterials capable of functional restoration in situ.

Extracellular Vesicles Delivered by a Nanofiber-Hydrogel Composite Enhance Healing In Vivo in a Model of Crohn's Disease Perianal Fistula.

Perianal fistulas represent a common, aggressive, and disabling complication of Crohn's disease (CD). Despite recent drug developments, novel surgical interventions as well as multidisciplinary treatment approaches, the outcome is dismal, with >50% therapy failure rates. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) offer potential therapeutic benefits for treating fistulizing CD, due to the pro-regenerative paracrine signals.

A new strategy for overcoming drug resistance in liver cancer: Epigenetic regulation.

Drug resistance in hepatocellular carcinoma has posed significant obstacles to effective treatment. Recent evidence indicates that, in addition to traditional gene mutations, epigenetic recoding plays a crucial role in HCC drug resistance. Unlike irreversible gene mutations, epigenetic changes are reversible, offering a promising avenue for preventing and overcoming drug resistance in liver cancer.

Efficacy and safety of low-dose cyclophosphamide combined with lenvatinib, pembrolizumab and TACE for unresectable hepatocellular carcinoma: A single-center, prospective, single-arm clinical trial.

Objective: Unresectable hepatocellular carcinoma (uHCC) continues to pose effective treatment options. The objective of this study was to assess the efficacy and safety of combining low-dose cyclophosphamide with lenvatinib, pembrolizumab and transarterial chemoembolization (TACE) for the treatment of uHCC.

Methods: From February 2022 to November 2023, a total of 40 patients diagnosed with uHCC were enrolled in this small-dose, single-center, single-arm, prospective study.