Novel 3D printed partial talar surface prosthesis for osteochondral lesions of the talar dome: A 2-year case report.

Rationale: Surgical treatment of osteochondral lesions of the talar dome (OLTD), for lesions larger than 15 mm2, surgical treatment remains challenging. To solve these problems, we presented a 3D-printed partial talar surface replacement (PTSR) technique for treating OLTD with severe lesions.

Patient Concerns: A 23-year-old male patient with OLTD experienced persistent pain for more than a year (Berndt and Harty classification V).

Lactobacillales derived from traditional Xizang dairy products improve insomnia and restore neurotransmitter-metabolic profiles via gut microbiota in PCPA-induced mice.

Insomnia remains a major public health challenge with limited safe treatment options. We demonstrate that mixed probiotics Lactobacillales (Lactobacillus helveticus L551, Lacticaseibacillus paracasei L578, and Streptococcus thermophilus S709, group P) alleviates insomnia in PCPA-induced mice. P significantly reduced sleep latency and increased sleep duration, while ameliorating anxiety behaviors.

The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.

A WRI-like gene (CeWRI3) homologous to AtWRI3/4 was isolated from oil-rich Cyperus esculentus tubers, which was shown to function in TAG accumulation through activating CeOLE2. In oilseeds, WRI1 has proven to be a master regulator in the transcriptional control of genes involved in fatty acid biosynthesis. By contrast, no evidence is available for WRI1 and its homologs in regulating genes associated with triacylglycerol (TAG) biosynthesis.

Nonlinear Association Between the Visceral-to-Subcutaneous Fat Area Ratio and the Risk of Hypertension in Prediabetic Individuals: Evidence From a Large-Scale Health Screening Dataset in China.

Abdominal fat distribution, particularly the visceral-to-subcutaneous fat area ratio (V/S ratio), offers a promising avenue for exploring the development of hypertension. However, there is a paucity of quantitative studies examining the relationship between the V/S ratio and the risk of hypertension in individuals with prediabetes, especially within the Chinese population. This cross-sectional study was conducted at the Health Management Center of Henan Provincial People's Hospital, involving 4071 participants with prediabetes.

Insights into membrane-bound fatty acid desaturase genes in tigernut (Cyperus esculentus L.), an oil-rich tuber plant in Cyperaceae.

Background: Tigernut (Cyperus esculentus L.), an oil-rich tuber plant of the Cyperaceae family, is typical for the naturally high content of oleic acid. However, to date, genes contributing to oil composition have not been well characterized.

Association between high-density lipoprotein cholesterol and 7-autoantibodies: a study on physical examination data from 2018 to 2023.

Background: Limited research has explored the effect of high-density lipoprotein cholesterol (HDL-C) on lung cancer's seven autoantibodies (7-AABs). This study investigated the association between serum HDL-C and 7-AABs among 5,574 Chinese adults aged ≥ 18 years from January 2018 to December 2023.

Methods: This cross-sectional study utilized physical examination data from the Department of Health Management at Henan Provincial People's Hospital.

Integrative Analysis Provides Insights into Genes Encoding LEA_5 Domain-Containing Proteins in Tigernut ( L.).

LEA_5 domain-containing proteins constitute a small family of late embryogenesis-abundant proteins that are essential for seed desiccation tolerance and dormancy. However, their roles in non-seed storage organs such as underground tubers are largely unknown. This study presents the first genome-scale analysis of the family in tigernut ( L.

Insights into the Stearoyl-Acyl Carrier Protein Desaturase (SAD) Family in Tigernut ( L.), an Oil-Bearing Tuber Plant.

Plant oils rich in oleic acid (OA) are attracting considerable attention for their high nutritional value and significant industrial potential. Stearoyl-acyl carrier protein desaturases (SADs) are a class of soluble desaturases that play a key role in OA accumulation in plants. In this study, the first genome-wide characterization of the gene family was conducted in tigernut ( L.

Diagnosis-related groups study of uterine leiomyoma patients based on E-CHAID.

Diagnosis-related groups (DRG) is emerging as an effective tool for measuring the quality and efficiency of medical services. This study aims to explore a DRG combination scheme for control the hospitalization costs in patients with uterine leiomyoma (UL). This study enrolled a total of 3087 UL patients from a tertiary hospital in Chongqing between year 2017 and 2021.

Association between visceral fat area and metabolic syndrome in individuals with normal body weight: insights from a Chinese health screening dataset.

Background: Metabolic syndrome (MetS) is increasingly diagnosed in individuals with normal body weight, and visceral fat emerges as a significant risk factor. However, the relationship between visceral fat area (VFA) and MetS within this population remains inadequately explored, and the diagnostic threshold for MetS in normal-weight individuals is yet to be established.

Methods: This study used a cross-sectional design combined with longitudinal cohort analysis.

Nonlinear Association Between the Liver Fat Content and the Risk of Hyperuricemia in Prediabetic Individuals: Evidence from Cross-Sectional Health Screening Data in China.

Purpose: The impact of hepatic lipid accumulation on hyperuricemia presents an intriguing research avenue, particularly in light of existing studies linking obesity with hyperuricemia. Nevertheless, there remains a scarcity of quantitative investigations into the correlation between liver fat content (LFC) and hyperuricemia among prediabetic cohorts, notably within the Chinese demographic.

Patients And Methods: This cross-sectional study was conducted at the Health Management Center of Henan Provincial People's Hospital between January 2019 and December 2023, involving 2,950 pre-diabetic participants.

Low-density lipoprotein cholesterol predicts coronary artery calcification events in patients with type 2 diabetes: a longitudinal study.

Background: Coronary Artery Calcification (CAC) is a major risk factor for various cardiovascular diseases. Low-Density Lipoprotein Cholesterol (LDL-C) is a significant factor in atherosclerotic cardiovascular diseases and is usually elevated in patients with Type 2 Diabetes Mellitus (T2DM). However, the association between LDL-C levels and incident CAC in asymptomatic T2DM patients remains unclear.

Baseline atherogenic index of plasma and its trajectory predict onset of type 2 diabetes in a health screened adult population: a large longitudinal study.

Background: The Atherogenic Index of Plasma (AIP) is a novel biomarker for assessing the severity of atherosclerosis and has been shown to be closely associated with the risk of Type 2 Diabetes Mellitus (T2DM). However, no prospective cohort study has comprehensively evaluated both the immediate risk stratification through baseline AIP and the long-term risk assessment through multi-time point AIP trajectories in health screened adults in relation to T2DM risk.

Methods: This longitudinal study included data from 42,850 participants who underwent health check-ups at Henan Provincial People's Hospital between January 2018 and August 2024.

Nonlinear association between liver fat content and lumbar bone mineral density in overweight and obese individuals: evidence from a large-scale health screening data in China.

Background: The impact of fatty liver disease on lumbar bone mineral density (BMD) represents an intriguing area of study, particularly in light of established research linking obesity to bone metabolism. However, there remains limited investigation into the correlation between quantifying liver fat content (LFC) and lumbar BMD among overweight and obese populations, particularly within the Chinese demographic. This study aims to accurately quantify LFC and investigate its association with lumbar BMD in overweight or obese individuals.

Association between the ratio of serum uric acid to high-density lipoprotein cholesterol and liver fat content: evidence from a Chinese health examination dataset.

Despite numerous studies investigating the correlation between the serum uric acid and high-density lipoprotein cholesterol ratio (UHR) and fatty liver disease, the evidence for the dose-response relationship between UHR and liver fat content (LFC) remains uncertain. This study employs quantitative computed tomography (CT) to quantify LFC and aims to investigate the correlation and dose-response relationship between UHR levels and LFC in Chinese adults. Based on the health check-up data from 2021 at Henan Provincial People's Hospital, China, the objective of this cross-sectional study was to investigate the association between UHR levels and LFC among individuals of different genders.

Three-dimensional digital model of the facial nerve assisted in the excision of benign parotid tumors based on 3D-DESS-WE-MRI.

This study proposes a novel surgical technique for the excision of benign parotid tumors, utilizing a extracapsular dissection guided by a three dimensional digital model of the facial nerve(3DFN-ECD) and compares its clinical efficacy with the extracapsular dissection (ECD) method. This prospective study included 68 patients with benign parotid tumors. The control group (40 patients) received the ECD treatment, while the experimental group (28 patients), underwent the 3DFN-ECD approach proposed in this study.

Insights into Genes Encoding LEA_1 Domain-Containing Proteins in , a Desiccation-Tolerant Tuber Plant.

LEA_1 domain-containing proteins constitute a class of late-embryogenesis-abundant proteins that are highly hydrophilic and predominantly accumulate in mature seeds. Though LEA_1 proteins have been proven to be essential for seed desiccation tolerance and longevity, little information is available on their roles in non-seed storage organs. In this study, a first genome-wide characterization of the gene family was conducted in tigernut ( L.

Molecular characterization of CeOLE6, a diverged SH oleosin gene, preferentially expressed in Cyperus esculentus tubers.

CeOLE6, a tuber-specific gene in tigernut, encodes a diverged SH oleosin that functions in oil accumulation via homo and heteromultimerization. Tigernut (Cyperus esculentus L.) is a rare example accumulating high levels of triacylglycerols (TAGs) in underground tubers; however, the mechanism underlying is poorly understood.

Nonlinear association between the triglyceride-glucose index and diabetes mellitus in overweight and obese individuals: a cross-sectional retrospective analysis.

Background: The triglyceride-glucose (TyG) index is linked to both the development and progression of diabetes, while obesity remains a significant risk factor for this disease. However, the relationship between the TyG index and overweight or obese diabetes remains unclear.

Methods: This study was a cross-sectional analysis of data from 40,633 participants with body mass index (BMI) ≥ 24 kg/m who were screened from January 2018 to December 2023 at Henan Provincial People's Hospital.

An evolved artificial radical cyclase enables the construction of bicyclic terpenoid scaffolds via an H-atom transfer pathway.

While natural terpenoid cyclases generate complex terpenoid structures via cationic mechanisms, alternative radical cyclization pathways are underexplored. The metal-catalysed H-atom transfer reaction (M-HAT) offers an attractive means for hydrofunctionalizing olefins, providing access to terpenoid-like structures. Artificial metalloenzymes offer a promising strategy for introducing M-HAT reactivity into a protein scaffold.

NSPs: chromogenic linkers for fast, selective, and irreversible cysteine modification.

The addition of a sulfhydryl group to water-soluble -alkyl(-nitrostyryl)pyridinium ions (NSPs) followed by fast and irreversible cyclization and aromatization results in a stable S-C sp-bond. The reaction sequence, termed Click & Lock, engages accessible cysteine residues under the formation of -hydroxy indole pyridinium ions. The accompanying red shift of >70 nm to around 385 nm enables convenient monitoring of the labeling yield by UV-vis spectroscopy at extinction coefficients of ≥2 × 10 M cm.

Nonlinear association between atherogenic index of plasma and type 2 diabetes mellitus in overweight and obesity patients: evidence from Chinese medical examination data.

Background: The atherogenic index of plasma (AIP) is closely associated with the onset of diabetes, with obesity being a significant risk factor for type 2 diabetes mellitus (T2DM). However, the association between the AIP and T2DM in overweight and obese populations has been infrequently studied. Therefore, this study aimed to explore this association in overweight and obese individuals with T2DM.

Association between liver fat level and risk of hypertension: evidence from a Chinese health examination dataset.

Background: Hypertension development is predominantly influenced by inflammation, excessive fat deposition, and metabolic irregularities. Among these factors, liver fat accumulation is a critical metabolic disorder. However, the quantification of liver fat levels and its associated risk for hypertension incidence remain ambiguous.

TIP aquaporins in Cyperus esculentus: genome-wide identification, expression profiles, subcellular localizations, and interaction patterns.

Background: Tonoplast intrinsic proteins (TIPs), which typically mediate water transport across vacuolar membranes, play an essential role in plant growth, development, and stress responses. However, their characterization in tigernut (Cyperus esculentus L.), an oil-bearing tuber plant of the Cyperaceae family, is still in the infancy.

Interplay between lipid dysregulation and ferroptosis in chondrocytes and the targeted therapy effect of metformin on osteoarthritis.

Introduction: Osteoarthritis (OA) is a devastating whole-joint disease affecting a large population worldwide; the role of lipid dysregulation in OA and mechanisms underlying targeted therapy effect of lipid-lowering metformin on OA remains poorly defined.

Objectives: To investigate the effects of lipid dysregulation on OA progression and to explore lipid dysregulation-targeting OA treatment of metformin.

Methods: RNA-Seq data, biochemical, and histochemical assays in human and murine OA cartilage as well as primary chondrocytes were utilized to determine lipid dysregulation.