E2-based mRNA vaccine encapsulated in lipid nanoparticles protects pigs against classical swine fever virus.

Unlabelled: Classical swine fever (CSF), caused by the classical swine fever virus (CSFV), remains a significant threat to the global pig industry. Recent advances in mRNA vaccines offered a promising platform for combating CSFV. In this study, we designed and evaluated three lipid nanoparticle (LNP)-encapsulated mRNA vaccine candidates encoding the ectodomain of E2 glycoprotein (E2_EX), E2_EX fused with the transmembrane (TM) region of the PEDV S protein (E2tm), and E2_EX fused with the TM region of the influenza virus HA protein (E2tm-HA).

Structural characterization of mRNA lipid nanoparticles (LNPs) in the presence of mRNA-free LNPs.

Lipid nanoparticles (LNPs) have emerged as a versatile platform for mRNA delivery across a range of applications, including disease prevention, cancer immunotherapy, and gene editing. Structural models of mRNA lipid nanoparticles (mRNA-LNPs) have also been proposed based on characterization of samples by using various advanced techniques. Among these, small angle neutron scattering (SANS) has proven essential for elucidating the lipid distribution within mRNA-LNPs, a factor crucial to both their preparation and efficacy.

Boosting the Oxygen Evolution Reaction by Tuning the Interfacial Iron Adsorption on Layered Double Hydroxide.

Understanding the interaction between ions in the electrolyte and electrode materials plays an important role in optimizing the water electrolysis performance for hydrogen production. Herein, the synergistic effect of iron (Fe) in the electrolyte and interlayer anions within the layered structure on the oxygen evolution reaction (OER) has been investigated by combining material synthesis with controlled structure, multiple characterization techniques, and first-principles calculations. Nickel aluminum layered double hydroxides (NiAl-LDHs) with different interlayer anions (CO, Cl, and Br) show similar oxygen evolution activity in the absence of Fe species in the electrolyte.

Single-atom molybdenum doping induces nickel oxide-to-hydroxide transformation for enhanced alkaline hydrogen evolution.

NiMoO compounds are widely regarded as among the most efficient non-noble metal catalysts for the hydrogen evolution reaction (HER). Nevertheless, understanding the structural evolution under conditions and further enhancing their performance remain key challenges. Herein, we report that single-atom Mo doping in NiO significantly enhances its HER activity, reducing the overpotential to 131 mV at 10 mA cm compared to undoped NiO.

Enzyme-Mimicking Active Site Clefts Demonstrated by Self-Assembled Peptide Nanoribbons with Polar Zippers.

Due to the inherent limitations of natural enzymes, biomimetic enzymes have received tremendous attention, among which those arising from peptide self-assembly are of particular interest due to their resemblance to natural enzymes in composition and hierarchical structures, as well as their structural robustness and designability. Despite considerable advances achieved in this area, it remains a major challenge to construct active site clefts through peptide self-assembly. Here, we report the design of polar zippers between peptide β-sheets to mimic the catalytic microenvironment of natural enzymes.

Heavy-Atom-Free Supramolecular Photosensitizers Derived from Conventional Fluorophores.

Heavy-atom-free photosensitizers (PSs) offer advantages such as efficient generation of reactive oxygen species (ROS), low dark cytotoxicity, good photostability, and high biocompatibility. Although the development of new PSs through organic synthesis has been a focus of active research, supramolecular chemistry offers a complementary pathway. This study presents a versatile supramolecular strategy to convert conventional fluorophores into heavy-atom-free PSs using a cyclic peptide-based scaffold that densely assembles fluorophore moieties.

Enhanced Graph Attention Network by Integrating Transformer for Epileptic EEG Identification.

Electroencephalography signal classification is essential for the diagnosis and monitoring of neurological disorders, with significant implications for patient treatment. Despite the progress made, existing methods face challenges such as capturing the complex dynamics of Electroencephalogram (EEG) signals and generalizing across diverse patient populations. In this study, the graph attention network and the transformer model are integrated for EEG signal classification, leveraging the enhanced capability to dynamically compute attention weights and adapt to the variable relevance of brain regions.

Chirality Inversion upon Coassembly of Stereoisomeric Short Peptides with Like-Handedness.

Despite numerous reports devoted to chirality inversion during the self-assembly of single chiral components, chirality inversion in the coassembly of two or more chiral components remains largely unexplored. Here we report the supramolecular chirality inversion via the coassembly of the two different stereoisomers of a minimalistic amphiphilic IK sequence with like-handedness in their self-sorting assembly. The coassembled nanofibrils exhibit noticeable helix inversion in a wide range of mixing ratios, compared to individual peptide nanofibrils.

Achieving Higher Activity of Acidic Oxygen Evolution Reaction Using an Atomically Thin Layer of IrO over CoO.

The development of electrocatalysts with reduced iridium (Ir) loading for the oxygen evolution reaction (OER) is essential to produce low-cost green hydrogen from water electrolysis under acidic conditions. Herein, an atomically thin layer of iridium oxide (IrO) has been uniformly dispersed onto cobalt oxide (CoO) nanocrystals to improve the efficient use of Ir for acidic OER. In situ characterization and theoretical calculations reveal that compared to the conventional IrO cluster, the atomically thin layer of IrO shows stronger interaction with the CoO and consequently higher OER activity due to the Ir-O-Co bond formation at the interface.

Transitioning Room-Temperature Phosphorescence from Solid States to Aqueous Phases via a Cyclic Peptide-Based Supramolecular Scaffold.

Aqueous room-temperature phosphorescence (RTP) materials have garnered considerable attention for their significant potential across various applications such as bioimaging, sensing, and encryption. However, establishing a universally applicable method for achieving aqueous RTP remains a substantial challenge. Herein, we present a versatile supramolecular strategy to transition RTP from solid states to aqueous phases.

A case of polyglucosan body myopathy caused by an RBCK1 gene variant and literature review.

Objective: To analyze the clinical and genetic characteristics of a patient with Polyglucosan body myopathy 1 (PGBM1) caused by a novel compound heterozygous variant in the RBCK1 gene.

Methods: The clinical data of the patient were collected, next-generation sequencing technology was used to determine the exome sequence of the patient, and the suspected pathogenic locus was verified by Sanger sequencing.

Results: Through whole-exome sequencing, we found that there were c.

Controlling Product Distribution of Polyethylene Hydrogenolysis Using Bimetallic RuM (M = Fe, Co, Ni) Catalysts.

Plastic hydrogenolysis is an attractive approach for producing value-added chemicals due to its mild reaction conditions, but controlling product distribution is challenging due to the formation of undesired CH This work reports several bimetallic RuM/CeO (M = Fe, Co, Ni) catalysts that shift the product of low-density polyethylene hydrogenolysis toward longer-chain hydrocarbons. These catalysts were characterized by using X-ray absorption fine structure spectroscopy, electron microscopy imaging, and H temperature-programmed reduction. The combined catalytic evaluation and characterization results revealed that the product distribution was regulated by the formation of bimetallic alloys.

A novel variant of DNM1L expanding the clinical phenotypic spectrum: a case report and literature review.

Background: Mitochondrial diseases are heterogeneous in terms of clinical manifestations and genetic characteristics. The dynamin 1-like gene (DNM1L) encodes dynamin-related protein 1 (DRP1), a member of the GTPases dynamin superfamily responsible for mitochondrial and peroxisomal fission. DNM1L variants can lead to mitochondrial fission dysfunction.

Bioinspired Self-Assembly of Metalloporphyrins and Polyelectrolytes into Hierarchical Supramolecular Nanostructures for Enhanced Photocatalytic H Production in Water.

Polypeptides, as natural polyelectrolytes, are assembled into tailored proteins to integrate chromophores and catalytic sites for photosynthesis. Mimicking nature to create the water-soluble nanoassemblies from synthetic polyelectrolytes and photocatalytic molecular species for artificial photosynthesis is still rare. Here, we report the enhancement of the full-spectrum solar-light-driven H production within a supramolecular system built by the co-assembly of anionic metalloporphyrins with cationic polyelectrolytes in water.

Case report of a child with long QT syndrome type 14 caused by CALM1 gene mutation and literature review.

Objective: To analyze the clinical and genetic characteristics of a patient with long QT syndrome type 14 (long QT syndrome-14, LQT14, OMIM # 616247) caused by a de novo CALM1 mutation.

Methods: The clinical data of the patient were collected, next-generation sequencing technology was used to determine the exome gene sequence of the patient, and the suspected pathogenic locus was verified by Sanger sequencing.

Results: A 5-year and 9-month-old girl was admitted to the hospital due to a syncopal episode.

Bimetallic-Derived Catalytic Structures for CO-Assisted Ethane Activation.

ConspectusIn recent years, the simultaneous upgrading of CO and ethane has emerged as a promising approach for generating valuable gaseous (CO, H, and ethylene) and liquid (aromatics and C3 oxygenates) chemicals from the greenhouse gas CO and large-reserved shale gas. The key challenges for controlling product selectivity lie in the selective C-H and C-C bond cleavage of ethane with the assistance of CO. Bimetallic-derived catalysts likely undergo alloying or oxygen-induced segregation under reaction conditions, thus providing diverse types of interfacial sites, e.

[Clinical and genetic analysis of five children with Catecholaminergic polymorphic ventricular tachycardia due to variants of RYR2 gene].

Objective: To explore the clinical and genetic characteristics of five children with Catecholaminergic polymorphic ventricular tachycardia (CPVT).

Methods: Five children with clinical manifestations consistent with CPVT admitted to the Department of Cardiology of Children's Hospital Affiliated to Zhengzhou University from November 2019 to November 2021 were selected as the study subjects. Their clinical data were collected.

CO electroreduction on single-atom copper.

Electroreduction of carbon dioxide (CO) or carbon monoxide (CO) toward C hydrocarbons such as ethylene, ethanol, acetate and propanol represents a promising approach toward carbon-negative electrosynthesis of chemicals. Fundamental understanding of the carbon─carbon (C-C) coupling mechanisms in these electrocatalytic processes is the key to the design and development of electrochemical systems at high energy and carbon conversion efficiencies. Here, we report the investigation of CO electreduction on single-atom copper (Cu) electrocatalysts.

[Clinical characteristics and genetic analysis of a child with Galactosemia due to compound heterozygous variants of GALT gene].

Objective: To explore the clinical features and genetic basis of a child with Galactosemia.

Methods: A child who had presented at the Children's Hospital Affiliated to Zhengzhou University on November 20, 2019 was selected as the study subject. Clinical data of the child was collected.

[Analysis of clinical characteristics and ACADM gene variants in four children with Medium chain acyl-CoA dehydrogenase deficiency].

Objective: To explore the clinical and genetic characteristics of four patients with medium-chain acyl-CoA dehydrogenase deficiency (MCADD).

Methods: Four children who had presented at the Children's Hospital Affiliated to Zhengzhou University between August 2019 and August 2021 were selected as the study subjects. Clinical data of the children were collected.

Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus.

African swine fever virus (ASFV) is highly contagious and can cause lethal disease in pigs. ASFV p72 protein is a major capsid protein that presents as trimer in the virion. Epitopes on the surface of p72 trimer are considered as protective antigens.

African swine fever virus I73R is a critical virulence-related gene: A potential target for attenuation.

African swine fever virus (ASFV) is a large, double-stranded DNA virus that causes a fatal disease in pigs, posing a threat to the global pig industry. Whereas some ASFV proteins have been found to play important roles in ASFV-host interaction, the functional roles of many proteins are still largely unknown. In this study, we identified , an early viral gene in the replication cycle of ASFV, as a key virulence factor.