Unraveling Qu-aroma variation between inner and outer layers of medium-temperature Daqu: A multi-omics and sensory approach.

Solid-state fermentation heterogeneity causes microenvironmental differences, shaping diverse microbial communities and metabolite compositions. Here, we aimed to investigate the variations in Qu-aroma between the inner (I-) and outer (O-) layers of medium-temperature Daqu (MT-Daqu) starter, and to analyze the differences in microbial communities as well as the associated metabolic pathways related to Qu-aroma volatile organic compounds (VOCs). Firstly, quantitative descriptive analysis revealed that the I-layer exhibited intensified rancid-roasted notes, whereas the O-layer showed stronger grain and woody notes.

Metabolic Activity Profiling of High-Temperature Microbiota Using Single-Cell Raman Spectroscopy and Deuterium Isotope Probing.

The microbial metabolic activity of , a key factor in production, varies due to environmental and raw material diversity. Quality differences exist among batches, even within the same fermentation room, and continue to change during storage. An accurate assessment of 's microbial activity is crucial for enhancing quality and production consistency, yet an in situ, nondestructive detection method remains elusive.

Hyaluronidases improve the hyaluronic acid yield during the fermentation of .

Background: Hyaluronic acid (HA), a linear acidic mucopolysaccharide with exceptional biocompatibility, is extensively utilized in pharmaceuticals and cosmetics. Industrial HA production predominantly relies on Streptococcus zooepidemicus fermentation. However, the accumulation of high-molecular-weight (HMW) HA increases broth viscosity, impeding nutrient diffusion and limiting yield.

Isolation of indigenous from aging tobacco leaves for improving the flavor of flue-cured tobacco.

Introduction: Aging of flue-cured tobacco is a slow microbial fermentation process that usually lasts for 2-3 years, which plays an important role in improving the quality of final products.

Methods: Re-dried tobacco leaves from seven Chinese regions were subjected to a controlled aging environment for 12 months. The bacterial community succession and volatile compounds dynamics in tobacco leaves throughout aging process were monitored.

Enhancement of oxaliplatin efficacy and amelioration of intestinal epithelial damage by GG through modulation of gut microbiota.

Background: Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality worldwide, necessitating extensive research into effective treatment strategies. Despite advancements in targeted therapies and immunotherapies, traditional chemotherapy remains the primary treatment modality for most patients. Here, we explored the synergy between GG (LGG), a probiotic, and the chemotherapeutic drug oxaliplatin (Oxp) in enhancing NSCLC treatment outcomes.

Adaptive responses and molecular mechanisms of Lactiplantibacillus plantarum to citric acid stress.

Lactiplantibacillus plantarum, known for its environmental adaptability due to genetic flexibility, was subjected to 180 days of citric acid exposure (a prevalent stressor derived from plants) to understand its adaptive response to environmental stress. The evolved strain demonstrated a 2.4-fold increase in cell density under 10 g/L citric acid stress, indicating significantly improved citric acid tolerance compared to the ancestral strain.

Strong-Flavor Baijiu Pit Mud Microbiome in the Context of Modern Industry: From a Black Box Under Empiricism to the Gradual Revelation of Microbial Ecosystems.

Chinese strong-flavor Baijiu (CSFB), one of the most popular categories in the alcoholic beverage market, is supposed to comply with the current industry development trend and achieve mechanization, modernization, and unification of production. Solid-state fermentation in the mud cellar is the fundamental characteristic of CSFB. Pit mud, which provides the necessary conditions for producing the precursors of crucial flavor substances, plays a decisive role in CSFB's characteristics and quality.

High-level secretory expression of recombinant type III human-like collagen α1 in Pichia pastoris via multilevel systematic optimization.

Collagen is the main component that makes up the internal structure of animals and is extensively used in several industrial fields including food, materials, chemicals, and pharmaceuticals. Despite the variety of preparation methods available, there is significant potential for enhancing the yield of recombinant collagen produced through engineered Pichia pastoris (P. pastoris).

Enhanced Biosynthesis of 6'-Sialyllactose in via Systematic Metabolic Engineering.

6'-Sialyllactose (6'-SL), a sialylated oligosaccharide belonging to human milk oligosaccharides (HMOs), finds extensive applications in food, pharmaceuticals, cosmetics, and particularly in infant nutrition and dietary supplements. In this study, K12 MG1655 was subjected to metabolic engineering to enhance 6'-SL biosynthesis. Initial pathway optimization involved the deletion of competing metabolic pathway genes (, , and ).

Rational design of phospholipase D to enhance thermostability and catalytic activity for efficient biocatalytic phosphatidylserine synthesis.

Phospholipase D (PLD) is an essential enzyme for the enzymatic synthesis of phosphatidylserine (PS), but its widespread application is limited by its low stability and activity. Herein, a highly active and thermostable multi-mutant PLD was obtained through a comprehensive strategy based on various computer-aided mutant design and systematic clustering analysis, extending its half-life from 3.7 to 89.

Evolution of aroma compounds in round soy sauce aroma type baijiu during aging and the effect of aging markers on the lasting aroma in finished glass.

Soy sauce aroma type Baijiu (SSAB) is a complex blend of seven rounds of raw SSAB, each with distinct aroma profiles that evolve during maturation. Tracking the aging of individual rounds is crucial for understanding flavor development. The round 1-4 SSABs were analyzed by gas chromatography-mass spectrometry and an electronic tongue over one-year aging.

Changes in flavor profile of sauce-flavor baijiu: Perceptual interactions between 1-propanol and aroma compounds.

1-Propanol is an important aroma compound in sauce-flavor Baijiu. However, the mechanism by which it affects the aroma of sauce-flavor Baijiu has not been fully investigated. In this study, an instrumental and perceptual analysis was employed to assess the impact of 1-propanol on the flavor and volatile compounds in sauce-flavor Baijiu.

Protein Engineering of Substrate Specificity toward Nitrilases: Strategies and Challenges.

Nitrilase is extensively applied across diverse sectors owing to its unique catalytic properties. Nevertheless, in industrial production, nitrilases often face issues such as low catalytic efficiency, limited substrate range, suboptimal selectivity, and side reaction products, which have garnered heightened attention. With the widespread recognition that the structure of enzymes has a direct impact on their catalytic properties, an increasing number of researchers are beginning to optimize the functional characteristics of nitrilases by modifying their structures, in order to meet specific industrial or biotechnology application needs.

Yeast community in the first-round fermentation of sauce-flavor Baijiu: Source, succession and metabolic function.

Yeasts play a crucial role in determining the quality and yield of sauce-flavor Baijiu, yet the source, succession, and metabolic functions of the yeast community in fermented grains during stacking fermentation remains unclear. In this study, amplicon sequencing combined with solid-state fermentation was used to investigate the structure and function of yeast community during the first-round fermentation of sauce-flavor Baijiu. The richness and diversity of yeast community increased throughout fermentation, with 83.

Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability.

The primary function of terminators is to terminate transcription in gene expression. Although some studies have suggested that terminators also contribute positively to upstream gene expression, the extent and underlying mechanism of this effect remain largely unexplored. Here, the correlation between terminating strength and upstream mRNA stability was investigated by constructing a terminator mutation library through randomizing 5 nucleotides, assisted by FlowSeq technology, terminator variants were categorized based on the downstream fluorescence intensity, followed by high-throughput sequencing.

A comprehensive review on microbial hyaluronan-degrading enzymes: from virulence factors to biotechnological tools.

Hyaluronan (HA), a natural high molecular weight polysaccharide, has extensive applications in cosmetology and medical treatment. Hyaluronan-degrading enzymes (Hyals) act as molecular scissors that cleave HA by breaking the glucosidic linkage. Hyals are present in diverse organisms, including vertebrates, invertebrates and microorganisms, and play momentous roles in biological processes.

Unlocking Green Biomanufacturing Potential: Superior Heterologous Gene Expression with a T7 Integration Overexpression System in .

Industrial biotechnology employs cells for producing valuable products and serving as biocatalysts sustainably, addressing resource, energy, and environmental issues. is a preferred host for creating microbial chassis cells and producing industrial enzymes and functional nutritional products. In this study, a dual-module T7 integration expression system in was established.

The efficient green bio-manufacturing of Vitamin K: design, production and applications.

Vitamin K, also known as methylnaphthoquinone, is a crucial fat-soluble nutrient necessary for the human body. The biological production of Vitamin K has received widespread attention due to its environmental friendliness and maneuverability in recent years. This review provides insights into the modular metabolic pathways of Vitamin K, lays the foundation for microbial metabolic flow balancing, cofactor engineering and dynamic regulation, and realizes the production of Vitamin K by synthesizing artificial cells from scratch.

Protein Engineering of Nicotinamide Riboside Kinase Based on a Combinatorial Semirational Design Strategy for Efficient Biocatalytic Synthesis of Nicotinamide Mononucleotides.

Industrial biosynthesis of β-nicotinamide mononucleotide (β-NMN) lacks a highly active nicotinamide riboside kinase for the phosphorylation process. Cumbersome preprocessing steps and excessive ATP addition contribute to its increased cost. To tackle these challenges, a docking combination simulation (DCS) semirational mutagenesis strategy was designed in this study, combining various modification strategies to obtain a mutant NRK-TRA with 2.

Multilevel Systematic Optimization To Achieve Efficient Integrated Expression of .

Genomic integration of heterologous genes is the preferred approach in industrial fermentation-related strains due to the drawbacks associated with plasmid-mediated microbial fermentation, including additional growth burden, genetic instability, and antibiotic contamination. Synthetic biology and genome editing advancements have made gene integration convenient. Integrated expression is extensively used in the field of biomanufacturing and is anticipated to become the prevailing method for expressing recombinant proteins.

Unraveling the intricacies of glycosaminoglycan biosynthesis: Decoding the molecular symphony in understanding complex polysaccharide assembly.

Glycosaminoglycans (GAGs) are extensively utilized in clinical, cosmetic, and healthcare field, as well as in the treatment of thrombosis, osteoarthritis, rheumatism, and cancer. The biological production of GAGs is a strategy that has garnered significant attention due to its numerous advantages over traditional preparation methods. In this review, we embark on a journey to decode the intricate molecular symphony that orchestrates the biosynthesis of glycosaminoglycans.

Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excess through gut-liver axis.

Diet is a key player in gut-liver axis. However, the effect of different dietary patterns on gut microbiota and liver functions remains unclear. Here, we used rodent standard chow and purified diet to mimic two common human dietary patterns: grain and plant-based diet and refined-food-based diet, respectively and explored their impacts on gut microbiota and liver.