Dynamic changes in physicochemical, bacterial, and volatile profiles during kombucha fermentation of mulberry fruits and leaves.

This study examined the dynamic changes in physicochemical properties, bacterial communities, volatile compounds, and electronic sensory profiles of kombucha produced from mulberry fruits (MFK) and mulberry leaves (MLK), compared to black tea (BTK) and green tea (GTK) over 12 days. Total flavonoid and polyphenol concentrations, along with antioxidant activity, increased during fermentation, peaked around day 6, and then declined. 16S rRNA sequencing identified Komagataeibacter rhaeticus and Komagataeibacter saccharivorans as the dominant bacterial species.

Evaluation of protein extraction methodologies on bacterial proteomic profiling: a comparative analysis.

Bacterial proteomics is a pivotal tool for elucidating microbial physiology and pathogenicity. The efficiency and reliability of proteomic analyses are highly dependent on the protein extraction methodology, which directly influences the detectable proteome. In this study, we systematically compared four protein extraction protocols-SDT lysis buffer with boiling (SDT-B), SDT lysis buffer with ultrasonication (SDT-U/S), a combination of boiling and ultrasonication (SDT-B-U/S), and SDT lysis buffer with liquid nitrogen grinding followed by ultrasonication (SDT-LNG-U/S)-to evaluate their effects on peptide and protein identification, distribution, and reproducibility in and .

Evaluation of dose and contact of a circular PFA catheter in a porcine model: Impact on hemolysis and renal function.

Background: Pulsed field ablation (PFA) is an innovative energy modality for cardiac ablation, but has been increasingly associated with hemolysis. While existing research primarily focuses on basket or flower-shaped catheters, data on circular PFA catheters remain limited.

Objective: The objective of this study was to evaluate the effects of dose and contact of a circular PFA catheter on hemolysis and renal function in a porcine model.

Ni-Bis(dithiolene) Coordination Enhanced Dual-Functional Covalent Organic Frameworks for both Cathodic Zn Storage and Anodic Zinc Deposition Control in Aqueous Zn-Ion Batteries.

In response to the increasing demand for sustainable energy storage solutions, aqueous zinc-ion batteries (AZIBs) have garnered significant attention for their high safety, low cost, and environmental friendliness. However, two major challenges to battery stability persist: developing efficient cathode materials and addressing zinc dendrite formation. Here, we report a dual-functional covalent organic framework (COF), named Ni-DAPTO, designed to improve both Zn storage on the cathode and zinc deposition kinetics on the anode.

A New Model for the Prediction of Intramyocardial Hemorrhage in ST-Segment Elevation Myocardial Infarction Patients After Emergency Percutaneous Coronary Intervention.

Background: Intramyocardial hemorrhage (IMH) after emergency percutaneous coronary intervention (PCI) in ST-segment elevation myocardial infarction (STEMI) patients is a significant predictor of major adverse cardiovascular events. However, current research lacks a simple and visual predictive model for IMH occurrence.

Aims: Our study aims to construct a Nomogram model to predict IMH occurrence.

Comparative proteomic analysis of casein micelles in human and small ruminant colostrum and mature milk using data-independent acquisition for precision improvement of infant formula.

Increasing attention has been directed toward improving infant formula derived from minor milk sources, given that human milk is widely regarded as the gold standard for infant nutrition. To bridge this gap, this study aims to conduct a comparative analysis of the casein micelle proteomes and particle sizes in human and small ruminant colostrum and mature milk. Colostrum and mature milk from humans, as well as goats and sheep, were collected.

Dynamic alterations of SEEG characteristics during peri-ictal period and localization of seizure onset zone.

Background: The evolution in peri-ictal period (from pre-ictal to ictal phase) of seizures contains abundant epileptogenic information, which aids in exploring the mechanism of seizures and localizing the epileptogenic zone (EZ). This study aims to investigate the regulatory mechanisms of seizure and localize EZ by analyzing the dynamic alterations of diverse characteristics during peri-ictal period based on SEEG.

Methods: A total of 61 patients with refractory focal epilepsy were included, and each patient underwent SEEG electrodes implantation.

Capillary electrophoresis-based detection of cow milk adulteration in specialty milks and products: Physicochemical characteristics of potential markers.

Specialty milks, known for their distinct nutritional profiles and expensive pricing, have received increased attention as a result of worries over their adulteration with cow milk. This study used capillary electrophoresis to detect cow milk adulteration (2%, 5%, 10%, 30%, and 50%) in raw, pasteurized, freeze-dried, and spray-dried forms of 7 specialty milks (buffalo, yak, goat, sheep, camel, horse, and donkey) and to identify particular markers derived from cow components. Cow α-CN, β-CN A1, β-LG, and α-LA were identified as potential markers for detecting cow milk adulteration in specific milk types, based on their retention times.

High-Entropy Doping Enabling Ultrahigh Power Density for Advanced Sodium-Ion Batteries.

Sodium-ion batteries (SIBs), owing to the plentiful sodium resources, are considered a viable large-scale energy storage substitute for lithium-ion batteries. Recently, NaV(PO)F (NVPF) has been increasingly investigated as an SIBs cathode material. However, the development of this cathode material is hindered by low intrinsic electronic conductivity, poor cycling stability at high rates, and low energy density.

Comprehensive analysis of species-specific differences in fatty acid composition and proteome of milk fat globules in human and animals.

Human milk, the gold standard for infant nutrition, precisely captures infant needs. This study analyzed the particle size distribution, fatty acid (FA) profile, and milk fat globule membrane (MFGM) proteome of milk fat globules (MFGs) in human milk and eight types of animal milks. Donkey and horse milks showed a greater abundance of smaller MFGs (0-5 μm).

Data-independent acquisition-based comparative analysis of whey proteomes in human colostrum, mature milk, and small ruminant milk for precision infant formula development.

Human milk is the gold standard for infant nutrition, evolving with developmental needs. This study identified 614 whey proteins from human, goat, and sheep colostrum and mature milk, with 76 conserved and 495 showing significant differences ( < 0.05).

Physicochemical properties and soluble whey protein composition of local and imported commercial ultra-high-temperature milk in China.

Ultra-high-temperature milk occupies a significant share of the Chinese dairy market, featuring various local and imported brands. This study examined the variations in physicochemical properties and whey proteomics among 10 local and imported milk brands available in China. The results showed that local brands exhibited a higher pH and a lower degree of protein structural denaturation compared with most imported brands.

Ultrasound combined with slightly acidic electrolytic water thawing improved rheological properties, quality and structure of mutton myofibrillar protein gel.

This study investigated the influence of ultrasound combined with slightly acidic electrolyzed water thawing (UST) on the rheological characteristics, quality and structure of mutton myofibrillar protein (MP) gel in comparison with other thawing methods (air thawing (AT), water immersion thawing (WT), microwave thawing (MT)). According to the result of rheological properties, the UST group had a higher storage modulus among thawing groups. UST treatment significantly improved the gel water holding capacity and whiteness (P < 0.

Comparison of bacterial intracellular and secreted proteins produced in milk versus medium for Escherichia coli by proteomic analysis.

The growth and reproduction of microorganisms are dependent on nutrient supply. Here, milk and Luria-Bertani (LB) media were used as nutrition sources for Escherichia coli, and the changes in bacterial and secretory proteins at 3 time points (3, 9, and 18 h) in the growth cycle were studied using a label-free proteomics technique. The findings revealed that the abundances of bacterial intracellular proteins inosine/xanthosine triphosphatase and universal stress protein F increase dramatically during the growth phase in milk and LB media.

Impact of Prolonged Cycle Length Resulting From Conversion of Atrial Fibrillation to Atrial Tachycardia on Ablation Outcome in Persistent Atrial Fibrillation Ablation.

There is limited available data regarding the impact of cycle length (CL) prolongation when converting atrial fibrillation (AF) to organized atrial tachycardia (AT) and its effect on clinical outcomes. We retrospectively screened and included a cohort of 132 patients with persistent or long-standing persistent AF who underwent circumferential pulmonary vein isolation (CPVI) and left atrial substrate modification (LASM) between January 2015 and October 2019. In all 132 consecutive patients, persistent AF was successfully converted into organized AT.

Proteomics and surface free fatty acid analysis of milk fat globules in spray- and freeze-dried bovine, goat, and horse milk powders.

Changes in the structure and composition of milk fat globules in spray- and freeze-dried milk powders have recently garnered notable attention. This study investigated changes in milk fat globular membrane (MFGM) proteins from bovine, goat, and horse milk powders, both spray- and freeze-dried, using a label-free proteomic approach, and quantified surface free fatty acids and their composition using GC. The results showed that several proteins, including α-CN and β-LG, increased, whereas fibrinogen α and β chain and mucin-1 decreased in the MFGM fractions of the studied spray-dried milk powders.

Dynamic changes in microbial communities and volatile compounds in kombucha fermentation using Flos sophorae and Elm fruits, compared to black and green tea.

The dynamic changes in physicochemical properties, microbial communities, and volatile compounds in kombucha made from Flos sophorae (FLSK) and Elm fruit (EFK) were compared to those of black tea (BTK) and green tea (GTK) over a 12-day fermentation period. The results revealed that overall flavonoid and polyphenol content, as well as antioxidant activity, increased initially and then decreased, accompanied by a steady reduction in pH within the fermentation broths investigated. Notably, the GTK exhibited stronger antioxidant activity than the other fermentation broths.

Impact of ultrasonic and heat treatments on the physicochemical properties and rennet-induced coagulation characteristics of milk from various species.

This study investigates the effects of heat and ultrasonic treatments on the physicochemical parameters and rennet-induced coagulation properties of milk from a variety of species, including cow, goat, buffalo, and donkey. Milk samples were subjected to heat treatments at different temperatures (65 °C, 80 °C, 90 °C, 100 °C) and ultrasonic treatment at varying power levels (200 W, 400 W, 600 W, 800 W, 1000 W). The results revealed that changes in turbidity, particle size, zeta potential, secondary structure, and surface hydrophobicity were altered by both ultrasonic and heat treatments, as well as the kind of milk.

The effect of non-insulin-based insulin resistance indices on the prediction of recurrence in patients with atrial fibrillation undergoing radiofrequency catheter ablation.

Background: Atrial fibrillation (AF) is acknowledged as a disease continuum. Despite catheter ablation being recommended as a primary therapy for AF, the high recurrence rates have tempered the initial enthusiasm. Insulin resistance (IR) has been established as an independent predictor for the onset of AF.

Unsupervised domain adaptation based on feature and edge alignment for femur X-ray image segmentation.

The gold standard for diagnosing osteoporosis is bone mineral density (BMD) measurement by dual-energy X-ray absorptiometry (DXA). However, various factors during the imaging process cause domain shifts in DXA images, which lead to incorrect bone segmentation. Research shows that poor bone segmentation is one of the prime reasons of inaccurate BMD measurement, severely affecting the diagnosis and treatment plans for osteoporosis.

Heterologous expression of the insect SVWC peptide WHIS1 inhibits invasion into A549 and HeLa epithelial cells.

(), a microbe commonly isolated from patients with vaginal tract infections, transforms from yeast to hyphae and produces many toxins, adhesins, and invasins, as well as biofilms resistant to antifungal antibiotic treatment. Effective agents against this pathogen are urgently needed. Antimicrobial peptides (AMPs) have been used to cure inflammation and infectious diseases.

Prevalence and genomic characterization of clinical Escherichia coli strains that harbor the plasmid-borne tet(X4) gene in China.

The tigecycline resistance gene tet(X4) has been widely reported in animals and animal products in some Asian countries including China in recent years but only sporadically detected in human. In this study, we investigated the prevalence and genetic features of tet(X4)-positive clinical E. coli strains.