Recapitulating dengue virus infection with human pluripotent stem cell-derived liver organoids for antiviral screening.

Dengue virus (DENV) poses a major global health threat, affecting an estimated 100 to 400 million people annually. The infection and pathogenesis remain incompletely understood, and no antiviral drug is currently approved for DENV treatment. Here, we develop a human pluripotent stem cell (hPSC)-derived liver organoid (hPLO) model to characterize DENV infection and screen for antivirals.

RNA-Seq Revealed the Effects of Cold Stress on Different Brain Regions of .

Cold shock represents a prevalent but harmful environmental stress factor that poses significant threats to fish survival and reproductive success. In fish, the brain acts as a central regulator of thermoregulatory processes. Nevertheless, how different brain regions respond molecularly to cold exposure remains largely unknown.

Gene-metabolite correlations underpin the hepatic fibrosis and metabolic disruption induced by methyltestosterone in juvenile Chinese longsnout catfish (Leiocassis longirostris).

Methyltestosterone (MT), a synthetic androgen frequently employed in aquaculture to induce sex reversal and boost growth rates, has yet to be extensively examined for its enduring effects on the health of fish. This study systematically evaluated the toxic effects of MT on the liver of Chinese longsnout catfish (Leiocassis longirostris) using an integrated approach of histopathology, transcriptomics, and metabolomics. The fish were subjected to three doses of MT (100 ng, 200 ng, and 300 ng) over 30 days, followed by pathological examinations of liver tissue.

RNA-seq revealed the effects of heat stress on different brain regions of .

Understanding how distinct brain regions of molecularly adapt to heat stress is vital for improving aquaculture sustainability and guiding conservation strategies in a warming climate. To elucidate the region-specific molecular mechanisms underlying heat stress responses in the brain of , we exposed L. longirostris to acute heat stress (32°C) for 24 h and performed RNA-seq and WGCNA on five brain regions (OB: olfactory bulb, FB: pituitary, hypothalamus, forebrain, MB: mesencephalon, CB: cerebellum, and SC: spinal cord).

Early brain gene network alterations and growth disruptions in juvenile Chinese longsnout catfish (Leiocassis longirostris) induced by 17β-estradiol and 17α-methyltestosterone.

Endocrine-disrupting chemicals (EDCs) 17β-estradiol (E2β) and 17α-methyltestosterone (MT) have been detected in aquatic environments at concentrations capable of perturbing neuroendocrine systems, yet their molecular mechanisms of action remain incompletely characterized. This study investigated the effects of chronic exposure to E2β and MT on brain development and neuroendocrine regulation in Leiocassis longirostris, a commercially important catfish species endemic to the Yangtze River Basin. Juvenile fish were exposed to three nominal dietary doses (100-300 ng) of each hormone for 30 days, corresponding to waterborne concentrations (556-1667 ng/L) within the upper range of environmental detections.

ARF4-mediated intracellular transport as a broad-spectrum antiviral target.

Host factors that are involved in modulating cellular vesicular trafficking of virus progeny could be potential antiviral drug targets. ADP-ribosylation factors (ARFs) are GTPases that regulate intracellular vesicular transport upon GTP binding. Here we demonstrate that genetic depletion of ARF4 suppresses viral infection by multiple pathogenic RNA viruses including Zika virus (ZIKV), influenza A virus (IAV) and SARS-CoV-2.

Tailoring Tumor Cell Golgi Apparatus-Targeting Self-Assembled Peptide for Effective Immunotherapy via Reshaping MIF-Mediated Immunosuppressive Network.

The immunosuppressive network formed by the enhanced crosstalk between tumor cells and various types of immune cells may ultimately lead to the formation of tumor immunosuppressive microenvironment (TIME). The Golgi apparatus (GA) of tumor cells is a key organelle in the formation of a tumor immunosuppressive network. However, there are no studies to show whether interfering with the GA of tumor cells can reshape the immunosuppressive network to enhance the effectiveness of immunotherapy.

[Characterization of host factors and upon Zika virus infection by construction of gene knockout mice].

The effects of host factors ADP-ribosylation factor 4 (ARF4) and ADP-ribosylation factor 5 (ARF5) upon Zika virus (ZIKV) infection were characterized by construction of gene knockout mice via CRISPR-Cas9. Firstly, and genes were modified by the CRISPR-Cas9 system and then microinjected into the fertilized eggs of C57BL/6JGpt mice. Fertilized eggs were transplanted to obtain or knockout (ARF4KO or ARF5KO) mice, and / double knockout mice were achieved by the mating between ARF4KO and ARF5KO mice (ARF4KO/ARF5KO).

A pilot study of the relationship between salivary microbial characteristics and endocrine and immune function in patients with burning mouth syndrome.

Objectives: To explore the potential role of oral bacteria in the pathogenesis of burning mouth syndrome (BMS).

Materials And Methods: Fifteen patients with BMS and 15 healthy controls were enrolled in this study. The relative abundance and diversity of salivary bacterial strains were analyzed using metagenomic DNA sequencing.

Integrating enzyme-nanoparticles bring new prospects for the diagnosis and treatment of immune dysregulation in periodontitis.

Enzymes play a significant role in mediating inflammatory and immune responses in periodontitis. Effective diagnosis, timely treatment, and continuous management of periodontal enzymes are essential to prevent undesirable consequences; however, this remains a significant challenge. Nanoparticles (NPs) have attracted significant attention in biomedicine because of their advantageous nanosized effects.

Extracellular vesicle-functionalized bioactive scaffolds for bone regeneration.

The clinical need for effective bone regeneration in compromised conditions continues to drive demand for innovative solutions. Among emerging strategies, extracellular vesicles (EVs) have shown promise as an acellular approach for bone regeneration. However, their efficacy is hindered by rapid sequestration and clearance when administered via bolus injection.

Immunogenicity and Protective Efficacy of Aerosolized Live-Attenuated Yellow Fever 17D Vaccine in Mice.

Yellow fever (YF), caused by the yellow fever virus (YFV), continually spreads and causes epidemics worldwide, posing a great threat to human health. The live-attenuated YF 17D vaccine (YF-17D) has been licensed for preventing YFV infection and administrated via the intramuscular (i.m.

Toxicological effects of Honokiol on zebrafish and its underlying mechanism.

The compound Honokiol, derived from the bark of Magnolia officinalis, possesses the ability to induce apoptosis and inhibit cellular damage caused by reactive oxygen species. The objective of this study was to investigate the toxicological and histopathological effects of Honokiol on zebrafish (Danio rerio) through conducting a semistatic acute toxicity test involving immersion in an Honokiol-containing solution. The results showed that the toxic effects of Honokiol on zebrafish were primarily manifested in the liver and gills.

Identification of TonB-dependent siderophore receptor inhibitors against using a structure-based high-throughput virtual screening method.

TonB-dependent siderophore receptors play a critical transport role for virulence formation and growth, and have become valuable targets for the development of novel antimicrobial agents. Traditional Chinese medicine has demonstrated notable efficacy in the treatment of fish diseases and includes potential antibacterial agents. Herein, we performed molecular docking-based virtual screening to discover novel TonB-dependent siderophore receptor inhibitors from traditional Chinese medicine and provide information for developing novel antibacterial agents.

Frailty and psychiatric disorders: A bidirectional Mendelian randomization study.

Background: The association between frailty and psychiatric disorders has been reported in observational studies. However, it is unclear whether frailty facilitates the appearance of psychiatric disorders or vice versa. Therefore, we conducted a bidirectional Mendelian randomization (MR) study to evaluate the causality.

Rapid development of double-hit mRNA antibody cocktail against orthopoxviruses.

The Orthopoxvirus genus, especially variola virus (VARV), monkeypox virus (MPXV), remains a significant public health threat worldwide. The development of therapeutic antibodies against orthopoxviruses is largely hampered by the high cost of antibody engineering and manufacturing processes. mRNA-encoded antibodies have emerged as a powerful and universal platform for rapid antibody production.

Causal association of lipoprotein-associated phospholipids on the risk of sepsis: a Mendelian randomization study.

Background: Many previous studies have revealed a close relationship between lipoprotein metabolism and sepsis, but their causal relationship has, until now, remained unclear. Therefore, we performed a two-sample Mendelian randomization analysis to estimate the causal relationship of lipoprotein-associated phospholipids with the risk of sepsis.

Materials And Methods: A two-sample Mendelian randomization (MR) analysis was performed to investigate the causal relationship between lipoprotein-associated phospholipids and sepsis based on large-scale genome-wide association study (GWAS) summary statistics.

The potential mechanism of concentrated mannan-oligosaccharide promoting goldfish's (Carassius auratus Linnaeus) resistance to Ichthyophthirius multifiliis invasion.

Because of the low host specificity, Ichthyophthirius multifiliis (Ich) can widely cause white spot disease in aquatic animals, which is extremely difficult to treat. Prior research has demonstrated a considerable impact of concentrated mannan-oligosaccharide (cMOS) on the prevention of white spot disease in goldfish, but the specific mechanism is still unknown. In this study, transcriptome sequencing, histological analysis, immunofluorescence analysis, phagocytosis activity assay and qRT-PCR assay were used to systematically reveal the potential mechanism of cMOS in supporting the resistance of goldfish (Carrasius auratus) to Ich invasion.

Immune Activation and Inflammatory Response Mediated by the NOD/Toll-like Receptor Signaling Pathway-The Potential Mechanism of Bullfrog () Meningitis Caused by .

is an emerging opportunistic pathogen that is highly pathogenic in both immunocompromised humans and animals. Once the disease occurs, treatment can be very difficult. Therefore, a deep understanding of the pathological mechanism of is the key to the prevention and control of the disease.

Ammonia nitrogen stress damages the intestinal mucosal barrier of yellow catfish () and induces intestinal inflammation.

Nitrogen from ammonia is one of the most common pollutants toxics to aquatic species in aquatic environment. The intestinal mucosa is one of the key mucosal defenses of aquatic species, and the accumulation of ammonia nitrogen in water environment will cause irreversible damage to intestinal function. In this study, histology, immunohistochemistry, ultrastructural pathology, enzyme activity analysis and qRT-PCR were performed to reveal the toxic effect of ammonia nitrogen stress on the intestine of .